Biosynthesis of lipases through solid substrate fermentation technique using Penicillium sp. isolated from pickle (MBL) 40
Paper Details
Biosynthesis of lipases through solid substrate fermentation technique using Penicillium sp. isolated from pickle (MBL) 40
Abstract
Lipases are important enzymes because of their various applications in different industries. Fungal lipases are used in many industries like Vitamins, amino acids fermentation, organic salts. Lipases have their own pH different from acids. The present study is concerned with production of lipase from locally isolated Penicillium sp. from pickle. Lipase showed its maximum activity (98.7+0.42U/ml) with teramera at 10%(v/w) inoculums after 72h of fermentation at the temperature of 30oC by using tap water as moistening agent when pH was maintained at 9 with 10g of substrate and 1% ammonium sulphate used as nitrogen source
Benjamin S, Pandey A. 1998.Candida rugosa lipases: molecular biology and versatility in biotechnology. Yeast. 14, 1069–87. http://dx.doi.org/10.1002/(SICI)10970061-19980915
Bradford JF. 1979. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principal of protein dye binding. Journal of Analytical Biochemistry 72, 1513-1523.
Edwinoliver NG, Thirunavukarasu K, Purushothaman S, Rose C, Gowthaman MK, Kamini NR. 2009. Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof. Journal of Agricultural and Food Chemistry 57, 10658–10663. http://dx.doi.org/10.1021/jf902726p.
Gracheva IM, Mukhamedzhanova TG, Voino LT. 1980. Regulation of lipase biosynthesis in the cultivation of Rhizopus oryzae. Biology khim 16, 303-305.
Haq IU, Idrees S, Rajoka MI. 2001. Production of lipase by Rhizopous oligosporous by solid state fermentation. Proces Biochemistry 37, 637-641.
Hillenga DJ, Versantvoort HJM, van der Molen S, Driessen AJM, Konings WN. 1995. Penicillium chrysogenum takes up the penicillin G precursor phenylacetic acid by passive diffusion. Applied and Environmental Microbiology 61, 2589 -2595.
Iftikhar T, Hussain A. 2002. Effect of nutrients on the extracellular lipase production by the mutant strain of R. oligosporous Tuv-31. Biotechnology 1(1), 15-20.
Iftikhar T, Niaz M, Zia MA, Haq IU. 2010. Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor. Brazilian Journal of Microbiology 41(4), 1124-1132.
Iwashita K. 2002. Recent studies of protein secretion by filamentous fungi. Journal of Bioscience and Bioengineering 94, 530–535.
kamini NR, Mala JGS, Puvanakrishnan R. 1998. Lipase production from Aspergillus niger by solid state fermentation using gingelly oil cake. Process Biochemistry 33, 505–511.
Kondo KY, Miura H, Sone K, Kobayashi, Iijima H. 1997. High level expression of a sweet protein, monellin, in the food yeast Candida utilis. Nature Biotechnology 15, 453-457.
Kundu AK, Pal N. 1970. Isolation of lipolytic fungi from the soil. Journal of Pharmacy Ind 32(4), 96-97.
Nadia N, Nehad ZA, El-sayyed AE, Hanan MA. 2010. Optimization of lipases synthesis by Mucor recemouses-production in a triple impeller bioreactor. Malaysian Journal of Microbiology 6(1), 7-15.
Pandey N, Dhakar K, Jain R, Pandey A. 2016. Temperature dependent lipase production from cold and pH tolerant species of Penicillium. Mycosphere (special issue), http://dx.doi.org/10.5943Mycosphere:si,3b,5.
Pommerville JC. 2001. Alcamo’s Fundamentals of Microbiology, Seventh Edition. Jones and Bartlet Publishers, Sudbury, Massachuctts Glendale Community College, Glendale, Arizona., 558-559.
Rajoka MI, Khan SH, Jabbar MA, Awan MS, Hashmi AS. 2006. Kinetics of batch single cell protein production from rice polishings with Candida utilis in continuously aerated tank reactors. Bioresource Technology 97, 1934-1941. http://dx.doi.org/10.1016/j.biortech.2005.08.019
Silva WOB, Mitidieri S, Schrank A, Vainstein MH. 2005. Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae. Process Biochemistry 40, 321-326.
Ushio K, Hirata T, Yoshida K, Sakaue M, Hirose C, Suzuki T, Ishizuk M. 1996. Super inducer for induction of thermostable lipase production by Pseudomonas species NT-163 and other Pseudomonas like bacteria. Biotechnology Techniques 10(4), 267-272.
Yaqoob P, Newsholme EA, Calder PC. 1999. Production of tumour necrosis factor-a by blood mononuclear cells increases with age and with BMI in healthy women. Process Nutrition Soc 58, 129A.
Shazia Malik, Tehreema Iftikhar, Mohammad Rafiq Khan, Hammad Majeed, Roheena Abdullah, 2016. Biosynthesis of lipases through solid substrate fermentation technique using Penicillium sp. isolated from pickle (MBL) 40. Int. J. Biosci., 9(6), 311-317.
Copyright © 2016 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.