Study of naturally sourced bacteria with antifungal activities

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Research Paper 01/08/2016
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Study of naturally sourced bacteria with antifungal activities

Femi Ayo Gbadeyan, Olukayode Olugbenga Orole, Gerard Uyi
Int. J. Micro. Myco.4( 1), 9-16, August 2016.
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Abstract

The study was carried out with the aim of sourcing for bacteria from the natural environment having antifungal capabilities to control and inhibit postharvest fungal spoilage of fruits and vegetables caused by Botrytis cinerea. Soil and water samples were collected from Heriot Watt University environment and Dr Ruth Fowler’s garden and inoculated using the spread plate technique; identification was carried out using Microbact Identification kits; and isolates assayed for antifungal activities against Botrytis cinerea. Forty eight bacteria species were isolated out of which sixteen (16) belonging to genera Pseudomonas, Bacillus, Escherichia, Burkholderia, Staphylococcus, Streptococcus, and Proteus showed antifungal activities. Bacteria species Pseudomonas stutzeri and Burkholderia cepacia had the highest zones of inhibition with average radii of 3.06 and 3.20 cm respectively. The bacteria had the potential to inhibit mycelial and spore growth at varying levels thus making them possible candidates for further tests and studies. Considering the aim of the study, further research into identifying these antifungal isolates inhibitory compounds and metabolites is highly recommended.

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Anderson PK, Cunningham AA, Patel NG, Morales FJ, Epstein PR, Daszak P. 2004. Emerging infectious diseases of plants: Pathogen pollution, climate change and agrotechnology drivers. Trends in Ecology and Evolution 19(10), 535–544.

Arrebola E, Sivakumar D, Korsten L. 2010. Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus. Biological Control 53, 122-128.

Aylor DE. 1990. The role of intermittent wind in the dispersal of plant pathogens. Annual review of phytopathology 28, 73-92.

Baiser B, Olden JD, Record S, Lockwood JL, McKinney ML. 2012. Pattern and process of biotic homogenization in the New Pangaea. Proceedings of the Royal Society B: Biological Sciences 279, 4772–4777.

Barkai Golan R. 2001. In: Postharvest diseases of fruits and vegetables. Development and control. Edited by Elsevier Science. Amsterdam Netherlands.

Baron JE. 2001. Rapid identification of Bacteria and Yeast: Summary of a National Committee for Clinical Laboratory Standard Proposed Guideline. Clinical Infectious Disease 33, 220-225.

Becker GS. 2009. U.S. food and agricultural imports: Safeguards and selected issues. CRS Report RL 34198.

Blackwell M. 2011. The fungi: 1, 2, 3…5.1 million species? American Journal of Botany 98(3), 426-438.

Blehert DS, Hicks AC, Behr M, Meteyer CU, Berlowski-Zier BM, Buckles EL, Coleman JTH, Darling SR, Gargas A, Niver R, Okoniewski JC, Rudd R.J, Stone WB. 2009. Bat white-nose syndrome: An emerging fungal pathogen? Science 323(5911), 227.

Brown JKM, Hovmøller MS. 2002. Aerial dispersal of pathogens on the global and continental scales and its impact on plant disease. Science 297(5581), 537-541.

Bryk H, Dyki B, Sobiczewski P. 2004. Inhibitory effect of Pseudomonas spp on the development of Botrytis cinerea and Penicillium expansum. Plant protect science 40(4), 128-134.

Buckley M. 2008. The Fungal Kingdom: Diverse and essential role in earth’s ecosystem. A report from the American Academy of Microbiology. Washington, DC: American Academy of Microbiology 1-44.

Canamas TP, Vinas I, Usall J, Torres R, Anguera M, Teixido N. 2008. Control of postharvest diseases on citrus fruit by pre-harvest applications of biocontrol agent Pantoea agglomerans CPA-2: part II. Effectiveness of Different Cell Formulations. Postharvest Biology Technology 49, 96-106.

Cuong ND, Nicolaisen MH, Sorensen J, Olson S. 2011. Hyphae colonizing Burkholderia sp. A New Source of Biological Control Agents against Sheath Blight Disease (Rhizoctonia solani AG1-IA) in Rice. Microbial Ecology 62(2), 425-434.

Dalié DKD, Deschamps AM, Richard-Forget F. 2010. Lactic acid bacteria– Potential for control of mould growth and mycotoxins: A review. Food Control 21(4), 370-380.

Donmez MF, Esitken A, Yildiz H, Ercisli S. 2011. Biocontrol of Botrytis cinerea on strawberry fruit by plant growth promoting bacteria. The Journal of Animal & Plant science 21(4), 758-763.

Droby S, Vinokur V, Weiss B, Cohen L, Daus A, Goldschmidt E, Porat R. 2002. Induction of resistance to Penicillium digitatum in grapefruit by the yeast biocontrol agent Candida oleophila. Phytopathology 92(4), 393-399.

Fisher MC, Henk DA, Briggs CJ, Brownstein JS, Madoff LC, McCraw SL, Gurr SJ. 2012. Emerging fungal threats to animal, plant and ecosystem health. Nature 484, 186-194.

Gachango E, Kirk W, Schafer R, Wharton P. 2012. Evaluation and comparison of biocontrol and conventional fungicides for control of postharvest potato tuber diseases. Biological Control 63(2), 115-120.

Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S, Di Venere D. 2011. Activity of extracts from wild edible herbs against postharvest fungal diseases of fruit and vegetables. Postharvest Biology and Technology 61(1), 72-82.

Jamalizadeh M, Etebarian HR, Aminian H, Alizadeh A. 2011. A review of mechanisms of action of biological control organisms against post-harvest fruit spoilage. Bulletin OEPP/EPPO Bulletin 41(1), 65-71.

Kulakiotu EK, Thanassoulopoulos CC, Sfakiotakis EM. 2004. Biocontrol of Botrytis cinerea by volatiles of ‘Isabella’ grapes. Phytopathology 94(9). 924-931.

Li X, Quan CS, Fan SD. 2007. Antifungal activity of a novel compound from Burkholderia cepacia against plant pathogenic fungi. Letters in Applied Microbiology 45, 508-514.

Lucon C, Guzzo S, de Jesus C, Pascholati S, de Goes A. 2010. Postharvest harpin or Bacillus thuringiensis treatments suppress citrus black spot in ‘Valencia’ oranges. Crop Protection 29(7), 766-772.

Mari M, Di Francesco A, Bertolini P. 2014. Control of fruit postharvest diseases: old issues and innovative approaches. Stewart postharvest Review 1(1), 1-4.

Martinez-Romero D, Serrano M, Bailen G, Guillen F, Zapata PJ, Valverde JM, Castillo S, Fuetes M, Valero D. 2008. The use of a natural fungicide as an alternative to preharvest synthetic fungicide treatments to control lettuce deterioration during postharvest storage. Postharvest Biology and Technology 47(1), 54-60.

Meziane H, Gavriel S, Ismailov Z, Chet I, Chernin L, Hofte M. 2006. Control of green and blue mould on orange fruit by Serratia plymuthica strains IC14 and IC1270 and putative modes of action. Postharvest Biology and Technology 39. 125-133.

Parke LJ, Gurian-sherman D. 2001. Diversity of Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annual review of phytopathology 39, 255-258.

Pennisi E. 2010. Armed and dangerous.Science 327, 804-805.

Prasanna ND, Vijayalakshmi K, Seshagirirao K, Shaheen SK. 2014. Characterization of antifungal compounds produced by Pseudomonas stutzeri (EGB3) isolated from gut of earthworm (Eiseniafoetida). Journal of Microbiology and antimicrobials 6(3), 57-65.

Quan CS, Zheng W, Liu Q, Ohta Y, Fan SD. 2006. Isolation and characterization of a novel Burkholderia cepacia with strong antifungal activity against Rhizoctonia solani. Applied Microbiolology and Biotechnology 72, 1276-1284.

Saima MK, RoohiI ZA. 2013. Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase. Journal of Genetic Engineering and Biotechnology 11, 39-46.

Sindhu SS, Gupta SK, Dadarwal KR. 1999. Antagonistic effect of Pseudomonas spp. on path-ogenic fungi and enhancement of growth of green gram (Vignaradiata). Biology and Fertility of Soils 29(1), 62-68.

Talibi I, Boubaker H, Boudyach EH, Ait B, Aoumar A. 2014. Alternative methods for the control of postharvest citrus diseases. Journal of Applied Microbiology 117(1), 1-17.

Taylor JW, Spatafora J, O’Donnell K, Lutzoni F, James T, Hibbett DS, Geiser D, Bruns TD, Blackwell M. 2004. The Fungi In Assembling the Tree of Life. Cracraft J, Donoghue MJ, editors. Oxford University Press 171-194.

Usall J, Smilanick J, Palou L, Denis-Arrue N, Teixido N, Torres R, Vinas I. 2008. Preventive and curative activity of combined treatments of sodium carbonates and Pantoea agglomerans CPA-2 to control postharvest green mold of citrus fruit. Postharvest Biology and Technology 50(1), 1-7.

Van Kan JAL. 2006. Licensed to kill: the lifestyle of a necrotrophic plant pathogen. Trends in Plant Science 11(5), 247-253.

Verma M, Brar SK, Tyagi R, Surampalli R, Valero J. 2007. Antagonistic fungi, Trichoderma spp.: Panoply of biological control. Biochemical Engendering Journal 37, 1-20.

Vitoratos A, Bilalis D, Karkanis A, Efthimiadou A. 2013. Antifungal Activity of Plant Essential Oils against Botrytis cinerea, Penicillium italicum and Penicillium digitatum. Notulae BotanicaeHorti Agrobotanic 41(1), 86-92.