DNA sequence analysis, morphology and pathogenicity of Fusarium oxysporum f. sp. lentis isolates inciting lentil wilt in Pakistan
Paper Details
DNA sequence analysis, morphology and pathogenicity of Fusarium oxysporum f. sp. lentis isolates inciting lentil wilt in Pakistan
Abstract
Fusarium wilt of lentil (Lens culinaris Medikus) incited by F. oxysporum f. sp. lentis causes huge lentil yield losses worldwide. Understanding morpho-molecular and pathogenic variation of F. oxysporum isolates is of utmost importance, which has not been previously studied in Pakistan. In this study, extensive two year (2011-12 and 2012-13) wilt surveys of seven districts viz. Chakwal, Jhelum, Gujrat, Sialkot, Layyah, Bhakkar and Khushab of Punjab, Pakistan were conducted, which revealed 100% mean disease prevalence and 25.7% mean incidence. Morphology assessment of recovered 105 isolates showed unique identification characters and variability in morphological measurements that differentiates F. oxysporum from other fusaria. Furthermore, sequencing of TEF-1α gene supported the morphological study and confirmed the associated fungi at species level. Phylogenetic analysis grouped all the selected type 31 isolates, obtained from different districts, under a single lineage within F. oxysporum species complex. Characterized isolates screened for pathogenicity revealed considerable pathogenic variability on two lentil genotypes (NARC-08-1 and Masoor-93). The inoculation of lentils confirmed the pathogenicity of 30 (96.77%) isolates on NARC-08-1 with 0 to 100% disease severity index and incidence with 19.33 to 100% yield reduction. On Masoor-93, 22 (70.97%) isolates were pathogenic with 0 to 66.66% severity index, 0 to 100% incidence and 6.47 to 53.68% yield reduction. Eight (25.81%) isolates viz. FWC15, FWJ35, FWG1, FWL6, FWL9, FWL12, FWB10 and FWK2 proved the most pathogenic resulting in highly virulent disease reaction (94.07% mean severity index and 100% mean incidence and yield reduction) and complete death of the NARC-08-1 seedlings.
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Khola Rafique, Chaudhary Abdul Rauf, Farah Naz, Ghulam Shabbir (2015), DNA sequence analysis, morphology and pathogenicity of Fusarium oxysporum f. sp. lentis isolates inciting lentil wilt in Pakistan; IJB, V7, N6, December, P74-91
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