Use of Im medium to overcome recalcitrance to somatic embryogenesis of improved cocoa genotypes (Theobrama cacao L.)
Paper Details
Use of Im medium to overcome recalcitrance to somatic embryogenesis of improved cocoa genotypes (Theobrama cacao L.)
Abstract
In vitro recalcitrance is the inability of plant cells, tissues and organs to respond to tissue culture manipulations. In cocoa (Theobroma cacao L.), recalcitrance is a limiting factor in tissue culture for the production of somatic embryos. This study aims to improve the production of somatic embryos by In Vitro culture of improved genotypes deemed recalcitrant to somatic embryogenesis. ‘’Im’’medium was used to induce the production of embryos of four enhanced and recalcitrant somatic embryogenesis genotypes encoded C8, C14, C15 and C16. The plant material consists of explants of petals and staminodes. The best results, which are characterized by levels of cal embryogenesis and the number of somatic embryos of 16% and 07, respectively, were obtained in the C14 genotype. This study showed that it is possible to produce somatic embryos in these improved varieties, thus eliminating the recalcitrance of these genotypes to somatic embryogenesis. The availability of improved material consisting of identical plants will allow the creation of more homogeneous plantations with high yields.
Driver JA, Kuniyuki AH. 1984. In vitro propagation of paradox walnut root stock. HortScience 19, 507-509.
Eliane MT, Modeste KK, André SB, Edmond KK, Mongomaké K. 2019. Effect of Water Stress Induced by Polyethylene Glycol 6000 on Somatic Embryogenesis in Cocoa (Theobroma cacao L.). Agricultural Sciences 10, 1240-1254.
Garcia C, Furtado AA, Costa M. 2019. Abnormalities in somatic embryogenesis caused by 2,4-D: an overview. Plant Cell, Tissue and Organ Culture, 137(2), 193-212. http://dx.doi.org/10.1007/s11240-019-01569-8
Garcia C, Corrêa F, Findley S. 2016. Optimization of somatic embryogenesis procedure for commercial clones of Theobroma cacao L. African Journal of Biotechnology 15(36), 1936-1951. http://dx.doi.org/10.5897/ajb2016.15513
Jane K, Siaka K, Lucien D, Georges N. 2017. Enhanced plantlet regeneration in two cacao (Theobroma cacao) Clones from Immature Inflorescence Explants. HORTSCIENCES, 52(6), 892–895. http://dx.doi.org/10.21273/HORTSCI11844-17
Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019. Induction of somatic embryos of recalcitrant genotypes of theobroma. Journal of Applied Biosciences 133, 13552–13560.
Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019. Use of mineral salts to remove recalcitrance to somatic embryogenesis of improved genotypes of cacao (Theobrama cacao L.). African Journal of Biotechnology 20(1), 33-42.
Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019. Carbon source and lifting of recalcitrance to the induction of somatic embryos in cacao (Theobroma cacao L.).International journal of biosciences 19(1), 75-84.
Kouassi KM, Manlé TE, Koné D, Soumahoro AB, Koné T, Kouablan KE, Koné M. 2017a. Effect of antioxidants on the callus induction and the development of somatic embryogenesis of cocoa [Theobroma cacao (L.)]. Australian Journal of Crop Sciences 11(1), 25-31. https://doi.org/10.21475/ajcs.2017.11.01.pne174
Kouassi KM, Kahia J, Kouame NC, Tahi GM, Kouablan KE. 2017. Comparing the effect of plant growth regulators on callus and somatic embryogenesis induction in four elite Theobroma cacao L. Genotypes. Hortsciences 52(1), 142–145. http://dx.doi.org/10.21273/HORTSCI110.92-16
Kouassi KM, Kouablan KE, Silué O, Tahi GM, Touré M, Konan KP. 2018.Comparison of systems combining auxins with thidiazuron or kinetin supplemented with polyvinylpirrolidone during embryogenic callus induction in three Theobroma cacao L. genotypes. Internatinal Journal Biological and Chemical Sciences 12(2), 804-811. https://doi.org/10.4314/ijbcs.v12i2.15
Sandra MMN, Ana MHR, Aura IUT. 2023. Propagation of the Colombian genotype of cacao (Theobroma cacao L.) CNCh-12 by somatic embryogenesis. Revis Bionatura 8(1), 26. http://dx.doi.org/10.21931/RB/2023.08.01.16.bd268
Koné Daouda, Thiémélé Deless Edmond Fulgence, Silué Oumar, Yao Saraka Didier Martial, N’Nan Alla Oulo, 2023. Use of Im medium to overcome recalcitrance to somatic embryogenesis of improved cocoa genotypes (Theobrama cacao L.). Int. J. Biosci., 23(4), 38-45.
Copyright © 2023 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.