Root crops based formulated culture media and its economic feasibility
Paper Details
Root crops based formulated culture media and its economic feasibility
Abstract
The study aimed to determine which among the formulated fungal culture media in dehydrated form utilizing cassava, (Manihot esculenta), sweet potato (Ipomoea batatas), ube (Dioscorea alata L.), taro (Colocasia esculenta), and potato (Solanum tuberosum) shall best support the growth of fungi; and to determine the most effective level of the root crop utilized as fungal culture media. The fungus, Saccharomyces cerevisiae, Aspergillus niger and Rhizopus stolonifer isolates were used in the study. Inocula of Rhizopus slotolonifer, accharomyces cerevisiae and Aspergillus niger were taken asceptically and using a sterilized inoculating needle, each plate was inoculated in the center through stab technique. The size of the colony was measured through its diameter using a digital Vernier caliper and expressed in millimeters. Based on the findings of the study, cassava, sweet potato, ube, taro and potato with dextrose and agar powder showed comparable effects on the growth of fungi under the study. A level of 75%, 85% and 95% of cassava, sweet potato, ube, taro and potato dextrose agar supported the growth of Saccharomyces cerevisiae, Aspergillus niger, and Rhizopus stolonifer. A concentration of 75%, 85% and 95% of cassava, sweet potato, ube, taro, and potato rootcrop with dextrose and agar powder can be formulated into fungal culture media. The different formulations in this study are cost effective in the cultivation of fungi such as Saccharomyces cerevisiae, Aspergillus niger and Rhizopus stolonifer.
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