Antibacterial activity of Cinnamomum verum essential oil against antibiotic-resistant Vibrio parahaemolyticus isolated from whiteleg shrimp (Litopenaeus vannamei)
Paper Details
Antibacterial activity of Cinnamomum verum essential oil against antibiotic-resistant Vibrio parahaemolyticus isolated from whiteleg shrimp (Litopenaeus vannamei)
Abstract
This study investigated multidrug-resistant (MDR) Vibrio parahaemolyticus strains and evaluated the antimicrobial activity of Cinnamomum verum (cinnamon bark) essential oil against these resistant strains, which are pathogenic to Pacific white shrimp (Litopenaeus vannamei). The results demonstrated that Cinnamomum verum essential oil exhibits significant antimicrobial activity against MDR V. parahaemolyticus. Specifically, the essential oil showed inhibition zone diameters ranging from 10 to 12 mm in disk diffusion assays, with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 12.5 µL/mL for both.
Balouiri M, Sadiki M, Ibnsouda SK. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Canillac N, Mourey A. 2001. Antibacterial activity of the essential oil of Picea excelsa on Listeria, Staphylococcus aureus and coliform bacteria. Food Microbiology 18(3), 261–268. https://doi.org/10.1006/fmic.2000.0397
CLSI. 2015. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria (3rd ed.). Clinical and Laboratory Standards Institute. https://clsi.org/standards/products/microbiology/documents/m45/
CLSI. 2021. Performance standards for antimicrobial susceptibility testing (31st ed.). Clinical and Laboratory Standards Institute. https://clsi.org/standards/products/microbiology/documents/m100/
Dang THO, Truong QP, Nguyen TP. 2022. Antibacterial resistance of Vibrio parahaemolyticus isolated from shrimp farms located in east coastal region of the Mekong Delta, Vietnam. Can Tho University Journal of Science 14(2), 1–7. https://doi.org/10.22144/ctu.jen.2022.018
Do DD, Le HA, Vu VV, Le NAD. 2023. Sustainable development in Vietnam shrimp industry: Challenges and solutions. Ho Chi Minh City University of Education Journal of Science 20(12). http://journal.hcmue.edu.vn/index.php/hcmuejos/article/view/145
Faikoh EN, Hong YH, Hu SY. 2014. Liposome-encapsulated cinnamaldehyde enhances zebrafish (Danio rerio) immunity and survival when challenged with Vibrio vulnificus and Streptococcus agalactiae. Fish & Shellfish Immunology 38(1), 15–24. https://doi.org/10.1016/j.fsi.2014.02.024
Hoque M, Bari ML, Juneja VK, Kawamoto S. 2008. Antimicrobial activity of cloves and cinnamon extracts against foodborne pathogens and spoilage bacteria, and inactivation of Listeria monocytogenes in ground chicken meat with their essential oils.
Jiang Y, Yao L, Li F, Tan Z, Zhai Y, Wang L. 2014. Characterization of antimicrobial resistance of Vibrio parahaemolyticus from cultured sea cucumbers (Apostichopus japonicas). Letters in Applied Microbiology 59(2), 147–154. https://doi.org/10.1111/lam.12258
Liyanage T, Madhujith T, Wijesinghe KGG. 2017. Comparative study on major chemical constituents in volatile oil of true cinnamon (Cinnamomum verum Presl. syn. C. zeylanicum Blum.) and five wild cinnamon species grown in Sri Lanka. Tropical Agricultural Research 28(3), 270. https://doi.org/10.4038/tar.v28i3.8231
Ngo TBV, Tran TTH, Phan TTA. 2021. To investigate antimicrobial activity of crude extract of Cleistocalyx operculatus leaves and initially make soluble powder. The University of Danang Journal of Science and Technology. https://jst-ud.vn/jst-ud/article/download/7512/5368/6851
Nguyen THN, Phan TTT, Nguyen TTL. 2020. Effects of Moringa leaf extracts on the survival rate and the resistant ability to acute hepatopancreatic necrosis disease on white leg shrimp. Journal of Vietnam Agricultural Science and Technology 3(112)/2020.
Nguyen TMH, Ho TA. 2017. Study on the antimicrobial activity of essential oils. Journal of Vietnam Agricultural Science and Technology 80(7), 57–63.
Nguyen TTH, Tran TH, Nguyen NP, Vo VT. 2022. Antibacterial activity against S. agalactiae of four essential oils of peppermint (Mentha piperita L.), lemongrass (Cymbopogon citratus), garlic (Allium sativum), and green tea (Camellia sinensis). International Journal of Biosciences (IJB) 21(6), 76–82.
Phu HH, Pham VK, Tran TH, Pham DTN. 2022. Extraction, chemical compositions and biological activities of essential oils of Cinnamomum verum cultivated in Vietnam. Processes 10(9), 1713. https://doi.org/10.3390/pr10091713
Quach VCT, Nguyen BT, Tu TD. 2024. The antibiotic resistance of Vibrio parahaemolyticus originated from intensively farmed white shrimp (Litopenaeus vannamei) in Ben Tre and Soc Trang provinces of the Mekong Delta, Vietnam. Veterinary Integrative Sciences 23(1). https://doi.org/10.12982/VIS.2025.024
Truong TH, Tran QKV, Ho TT, Doan QT, Tran NH. 2023. Antibacterial activity of Syzygium nervosum extracts against Vibrio parahaemolyticus, causing acute hepatopancreatic necrosis in white leg shrimps (Litopenaeus vannamei). Hue University Journal of Science: Agriculture and Rural Development 132(3D). https://doi.org/10.26459/hueunijard.v132i3D.7324
Thu Nguyen Ngoc, Yen Tran Thi Ngoc, Duong Tran Anh, Y Thai Nhu, Van-Thanh Vo (2025), Antibacterial activity of Cinnamomum verum essential oil against antibiotic-resistant Vibrio parahaemolyticus isolated from whiteleg shrimp (Litopenaeus vannamei); JBES, V26, N4, April, P53-60
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