Optimization of direct shoot induction using cytokinin and auxin young leaf explants of Enicostemma littorale Blume.
Paper Details
Optimization of direct shoot induction using cytokinin and auxin young leaf explants of Enicostemma littorale Blume.
Abstract
We report here a robust in vitro Reincarnation that is repeatable protocol for the medicinally important plant Enicostemma littorale Blume. Young leaf explants were employed to further develop direct shoot induction, focusing mainly on optimal plant growth regulator combinations and preferred concentrations. All experiments were performed in MS baseline substrate. Numerous meditations on the cytokinins 6-Benzylaminopurine (BAP) and kinetin (KN) were used singly or in concert to cause precocious shoot emergence. The highest number of shoot regeneration was observed over MS medium fortified with 2.22 µM BAP+2.32 µM KN with the highest frequency of shoot induction (92) and maximum number of shoots/explant (20). In contrast, calli production unusual was observed and formed with elevated levels of (BAP (8.88 µM) and KN (4.64 µM), which may be due to a threshold effect which restricted a direct shoot organogenesis. NAA: Induction of roots was tested at different concentrations of auxin naphthaleneacetic acid (NAA). Approximately fifteen roots per shoot were obtained on half-strength MS media containing 1.22 µM NAA (100 % rooting percentage). Downstream research involving the development of the propagation, conservation, and gene improvement of E. littorale at a large scale is expected to be expedited by the rapid and efficient regeneration process of the established protocol. These results are the basis for potential biotechnological applications of this medicinally pure species in the future and emphasize the importance of hormone profile in the context of positive direct organogenesis.
Achar SS, Vinjamuri S. 2021. A simple and efficient micropropagation protocol for developing plantlets of Exacum bicolor Roxb.- An endangered, ornamental, and antidiabetic herb. Asian Journal of Pharmaceutical and Clinical Research. https://doi.org/10.22159/ajpcr.2021.v14i5.40966
Bhojwani SS, Dantu PK. 2013. Plant tissue culture: An introductory text. Springer Nature. https://doi.org/10.1007/978-81-322-1026-9
Cardoso JC, Silva JAT. 2013. Gerbera micropropagation. Biotechnology Advances 31(8). https://doi.org/10.1016/j.biotechadv.2013.05.008
Debergh P, Maene L. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Horticulturae 14(4), 335. https://doi.org/10.1016/0304-4238(81)90047-9
Eevers N, Gielen M, Sánchez-López AS, Jaspers S, White JC, Vangronsveld J, Weyens N. 2015. Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media. Microbial Biotechnology 8(4), 707. https://doi.org/10.1111/1751-7915.12291
Hasnain A, Naqvi SAH, Ayesha SI, Khalid F, Ellahi M, Iqbal S, Hassan MZ, Abbas A, Adamski R, Markowska D, Baazeem A, Mustafa G, Moustafa M, Hasan ME, Abdelhamid MMA. 2022. Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches. Frontiers in Plant Science 13. https://doi.org/10.3389/fpls.2022.1009395
José F, R ASK. 2015. Induction of callogenesis and shoot regeneration of a medicinal plant species Peristrophe bicalyculata (Retz.) Nees. Kongunadu Research Journal 2(2). https://doi.org/10.26524/krj95
Lone SM, Hussain K, Malik A, Magray M, Hussain SM, Rashid M, Farwah S. 2020. Plant propagation through tissue culture- A biotechnological intervention. International Journal of Current Microbiology and Applied Sciences 9(7). https://doi.org/10.20546/ijcmas.2020.907.254
Parvin J, Robbani M, Hasan MF, Hoque F. 2018. Standardization of plant growth regulators for successful tissue culture of sweet potato. Journal of the Bangladesh Agricultural University 16(2), 178. https://doi.org/10.3329/jbau.v16i2.37957
Pathak AR, Joshi AG. 2015. Indirect organogenesis from leaf explants of Hemidesmus indicus (L.) R. Br.: an important medicinal plant. https://www.tandfonline.com/doi/full/10.1080/11263504.2015.1108938
Pierik R, Jansen J, Maasdam A, Binnendijk CM. 1975. Optimalization of Gerbera plantlet production from excised capitulum explants. Scientia Horticulturae 3(4). https://doi.org/10.1016/0304-4238(75)90049-7
Qu L, Chen J, Henny RJ, Huang Y, Caldwell RD, Robinson CA. 2002. Thidiazuron promotes adventitious shoot regeneration from pothos (Epipremnum aureum) leaf and petiole explants. In vitro Cellular & Developmental Biology- Plant 38(3). https://doi.org/10.1079/ivp2001270
Rajamani S, Thirumalai T, Hemalatha M, Balaji R, David E. 2013. Pharmacognosy of Enicostemma littorale: A review. Asian Pacific Journal of Tropical Biomedicine 3(1). https://doi.org/10.1016/s2221-1691(13)60028-3
Roy SP, Niranjan CM, Jyothi T, Shankrayya MM, Vishawanath KM, Prabhu K, Gouda V, Setty RS. 2010. Antiulcer and anti-inflammatory activity of aerial parts Enicostemma littorale Blume. Journal of Young Pharmacists 2(4). https://doi.org/10.4103/0975-1483.71629
Swarts A, Matsiliza-Mlathi B, Kleynhans R. 2018. Rooting and survival of Lobostemon fruticosus (L.) H. Buek stem cuttings as affected by season, media and cutting position. South African Journal of Botany 119, 80. https://doi.org/10.1016/j.sajb.2018.08.019
Talla SK, Madam E, Manga S, Aileni M, Mamidala P. 2018. Efficient TDZ-induced regeneration from capitulum explants of Gerbera jamesonii Bolus ex Hooker F., an ornamental plant with high aesthetic value. Plant Biosystems 153(5). https://doi.org/10.1080/11263504.2018.1539040
Twaij BM, Jazar ZH, Hasan MN. 2020. Trends in the use of tissue culture, applications and future aspects. International Journal of Plant Biology 11(1). https://doi.org/10.4081/pb.2020.8385
Chandran Sureshpandian, Gandhi Premkumar, G. Mahendraperumal, N. Nirmal Kumar, 2025. Optimization of direct shoot induction using cytokinin and auxin young leaf explants of Enicostemma littorale Blume.. J. Biodiv. Environ. Sci., 27(1), 1-12.
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