Plant growth promoting and biocontrol activity of Rhizobium meliloti against plant pathogens

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Research Paper 19/07/2025
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Plant growth promoting and biocontrol activity of Rhizobium meliloti against plant pathogens

R. Nithyatharani, S. Subashini, M. Vinoth, R. Krishnan
Int. J. Biosci. 27(1), 336-342, July 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

The present study was carried out with plant pathogenic microorganisms were isolated from the infected roots of Vigna mungo plant, collected from Thanajavur district, Tamil Nadu, India. The identified fungal colonies were confirmed as Aspergillus flavus and Fusarium solani. In this study plant growth promoting rhizobacterial strains were successfully isolated from the Vigna mungo rhizosphere soil. The isolated organisms were confirmed as Rhizobium meliloti. The isolated organisms were used for indole acetic acid production the isolated organism were produced high level. The seed germination test were analysed in agar plates. The Vigna mungo seeds were treated with isolated Rhizobial species and placed on agar surface the seed germination was counted 90% percentage seeds were germinated in this results. Analysis the Morphometric and Biochemical parameters, After 10 days of growth, germination percentage, root length, shoot length, fresh weight and dry weight were measured and calculated. Biochemical compound such as chlorophyll, total protein and total carbhohydrate were analysed. The biochemical contents such as chlorophyll, protein and carbohydrates content were notied in decreased amountthe in treatment T6 (Aspergillus flavus and Fusarium solani) containing pot, when compared with control treatment (without microorganisms).

Arnon DI. 1949. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiology 24, 1.

Ashrafuzzaman M, Hossen MA, Ismail MR, Hoque MA, Islam SMZ, Shahidullah, Meron S. 2009. Efficiency of plant growth promoting rhizobacteria (PGPR) for the enhancement of rice growth. African Journal of Biotechnology 8(7), 1247–1252.

Biswas JC, Ladha LK, Dazzo FB. 2000. Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Journal of Soil Science 64, 1644–1650.

Bowen N, Rovira Z. 1999. Biology and control of the downy mildews of pearl millet, sorghum and finger millet. Mysore: Wesley Press, 304.

Bric JM, Bustock RM, Silversone SE. 1991. Rapid in situ assay for indole acetic acid production by bacterial immobilization on a nitrocellulose membrane. Applied and Environmental Microbiology 57, 534–538.

Davis S, Balachandar D, Mohanasundaram K. 2005. Studies on synergism between Rhizobium, PGPR and phosphate solubilizing bacteria in blackgram. In: Kannaiyan S, Kumar K, Govimdarajan K (eds.), Biofertilizer Technology for Rice Based Cropping System. Scientific Publishers, Jodhpur, 269–273.

Dey R, Pal KK, Bhatt DM, Chauhan SM. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of PGPR. Microbiological Research 159, 371–394.

Glick BR. 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology 41, 109–117.

Hoffland F, Saxena AK, Gopal M, Tilak KVBR. 1996. Effects of co-inoculation of plant growth promoting rhizobacteria and Bradyrhizobium sp. (Vigna) on growth and yield of green gram (Vigna radiata L. Wilczek). Journal of Tropical Agriculture 80, 28–35.

Jetiyanon K, Kloepper JW. 2002. Mixtures of plant growth promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control 24, 285–291.

Kloepper JW, Rodriguez-Kabana R, Kenney DS, Reddy MS, Martinez-Ochoa N, Kokalis-Burelle N, Arthur K. 2004. Development of an integrated biological approach to develop transplants suppressive to various plant diseases. Phytopathology 89, S40.

Kloepper JW, Rodriguez-Ubana R, Zehnder GW, Murphy JF, Sikora E, Fernandez C. 1991. Plant root bacterial interactions in biological control of soil-borne diseases and potential extension to systemic and foliar diseases. Australasian Plant Pathology 28, 21–26.

Kloepper JW, Schroth MN. 1978. Plant growth promoting rhizobacteria on radishes. In: Station de Pathologie Végétale et Phytobactériologie (ed.), Proceedings of the 4th International Conference on Plant Pathogenic Bacteria II. Tours: Gilbert-Clary, 879–882.

Kloepper JW. 1993. Plant growth-promoting rhizobacteria as biological control agents. In: Metting FB (Ed.), Soil Microbial Ecology: Applications in Agricultural and Environmental Management. New York: Marcel Dekker, 255–274.

Lalande R, Bissonnette N, Coutlee D, Antoun H. 1989. Identification of rhizobacteria from maize and determination of their plant-growth promoting potential. Plant Soil 115, 7–11.

Liu JF, Zehnder GW, Schuster DJ, Sikora EJ, Polston JE, Kloepper JW. 1992. Plant growth promoting rhizobacteria mediated protection in tomato against tomato mottle virus. Plant Disease 84, 779–784.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193(1), 265–275.

Pradhan N, Sukla LB. 2006. Solubilization of inorganic phosphates by fungi isolated from agricultural soil. African Journal of Biotechnology 5, 850–854.

Shanmugaiah V, Manoharan PT, Rajendran A, Mathivanan N. 2009. Growth promotion and suppression of phytopathogens in green gram (Vigna radiata L.) by Trichoderma viride and Pseudomonas fluorescens. Journal of Indian Botanical Society 4(1), 99–110.

Somasegaran P, Hoben HJ. 1994. Handbook of Rhizobia: Methods in Legume-Rhizobium Technology. Springer-Verlag, New York.

Vincent JM. 1970. A Manual for the Practical Study of Root-Nodule Bacteria. IBP Handbook No. 15. Oxford: Blackwell, 73–97.

Wei S, Turkevitch E, Okon Y. 1996. Recent advances in the use of plant growth promoting rhizobacteria (PGPR) in agriculture. In: Subba Rao NS, Dommergues YR (eds.), Microbial Interaction in Agriculture and Forestry. Vol. 2. Enfield (NH), USA: Science Publishers Inc., 229–249.

Yanni YG, Reddy MS, Wang Q, Mei R, Kloepper JW. 1997. Role of rhizobacteria in tomato early blight control. Phytopathology 89, S87.

Zar JH. 1984. Biostatistical Analysis. Englewood Cliffs, NJ: Prentice Hall, Inc., 437–467.

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