Paper Details

Saccharomyces cerevisiae as a model for the evaluation of dihydropyridine calcium antagonist effects

Asma Cherait, Mohamed Reda Djebar, Houria Berrebbah

DOI: https://dx.doi.org/10.12692/ijb/4.12.108-116

Int. J. Biosci. 4(12), 108-116. June, 2014. (PDF)

Abstract:

In this work, we are interested to using Saccharomyces cerevisiae as eukaryotic model and an alternative one to animal testing for bringing out the effect of nifedipine, a dihydropiridine L-type calcium channel blocker. Thus, a culture of Saccharomyces cerevisiae was treated with different concentrations of this xenobiotic (0.01, 0.05, 0.5, 1 mM) during 3 hours. After that we measured the levels of some stress biomarkers (GSH, GST and peroxidases activities). The results obtained show a significant/high significant increase in all studied biomarkers following treatment with a dose-response manner. These data highlight an oxidative stress induced by the presence of the calcium antagonist, and expressed by a stimulation of the oxidative enzymes activities