Improving the microbiological quality of spices and spice blends using treatments accessible to SMEs/SMIs
Paper Details
Improving the microbiological quality of spices and spice blends using treatments accessible to SMEs/SMIs
Abstract
The aim of this study is to contribute to the safety of spices and spice blends through the use of simple treatments that can be applied in SMEs/SMIs. To this end, samples of fresh onion bulbs, par sley, ginger, chili peppers, turmeric, and garlic were collected for the production of treated and untreated powdered spices. Samples of powdered spices of the same type were also purchased commercially in order to compare the results. Four types of heat treatment were applied depending on the nature of the spice. These were blanching, roasting, oven heating, and tyndallization. Microbiological analyses were carried out using standardized methods and covered all samples. The moisture content of the spices was also determined. An overall assessment of the results of the analyses of total mesophilic aerobic flora, yeasts and molds, Staphylococcus aureus, sulfite-reducing Clostridium, Bacillus cereus, Escherichia coli, and Salmonella shows that, according to microbiological quality criteria, 75. 00 % of untreated powdered spices are of unsatisfactory quality, as are 71.43 % of commercial powdered spices. The treatments resulted in a reduction of between 0.06 and 6.15 log CFU/g, depending on the bacteria. This reduction resulted in satisfactory quality for 62.50 % of the treated spices and acceptable quality for 37.50 %. Blanching and roasting resulted in an acceptable quality blend of spices. However, heating in an oven did not significantly improve the microbiological quality of the spice blend. These simple treatments blanching and roasting could be applied in SMEs/SMIs processing companies to reduce the microbial load in spices and spice blends.
Abd El-Rahman MA. 2019. Microbiological Quality and Heavy Metals Content of some Spices and Herbs Kinds. Journal of Food and Dairy Sciences 10, 237-241. https://doi.org/10.21608/jfds.2019.53499.
ANSE 2022. Description sheet for foodborne biological hazards: Staphylococcus aureus and Staphylococcal enterotoxins Reference No. 2016-SA-0076, 4p.
Arcos-Limiñana V, Prats-Moya S, Maestre-Pérez S. 2025. Impact of a UV-C Scalable Reactor on the Chemical and Sensory Quality of Peppercorns. Foods 14, 1-17. https://doi.org/10.3390/foods14173056.
Banerjee M, Sarkar PK. 2003. Microbiological quality of some retail spices in India. Food Research International 36, 469‑474. https://doi.org/10.1016/S0963-99690200194-1 .
Benayad B, Meziane N. 2021. Reliability assessment of sterilization systems and detection of recalcitrant microorganisms. Professional Master’s Thesis. Biological Sciences, Frères Mentouri Constantine I University. Algerian Republic, 83p.
CAC/RCP 42 1995. Code of Hygienic Practice for Spices and Dried Aromatic Herbs, Codex Alimentarius, WHO, FAO,16p.
Cicero N, Gervasi T, Durazzo A, Lucarini M, Macrì A, Nava V, Giarratana F, Tardugno R, Vadalà R, Santini A. 2022. Mineral and Microbiological Analysis of Spices and Aromatic Herbs. Foods 11, 1-12. https://doi.org/10.3390/foods11040548
Clem MF. 1992. Sterilization and antiseptics in Auer J.A., Equine surgery, Philadelphia: Saunders, p. 102-110.
CXS-347 2019. Standard for dried or dehydrated garlic. Codex Alimentarius, 5p.
CXS-353 2022. Standard for dried or dehydrated chili peppers and paprika, Codex Alimentarius, 6p.
CXS-359 2024. Standard for spices derived from dried or dehydrated roots, rhizomes, and bulbs – turmeric, Codex Alimentarius, 6p.
Fernandes A, Ramos C, Monteiro V, Santos J, Fernandes P. 2022. Virulence Potential and Antibiotic Susceptibility of S. aureus Strains Isolated from Food Handlers. Microorganisms 10, 1-12. https://doi.org/10.3390/microorganisms10112155.
Fessard A. 2017. Search for indigenous lactic acid bacteria capable of fermenting tropical fruits with increased antioxidant activity. Doctoral thesis, University of Réunion, Île de la Reunion, 192p.
Food and Drug Administration FDA. 2013. Draft Risk Profile: Pathogens and Filth in Spices. Report, 213p.
Garbowska M, Berthold-Pluta A, Stasiak-Różańska L. 2015. Microbiological quality of selected spices and herbs including the presence of Cronobacter spp. Food Microbiology 49, 1-5. http://dx.doi.org/10.1016/j.fm.2015.01.004.
Garcia S, Iracheta F, Galvain F, Heredia N. 2001. Microbiological Survey of Retail Herbs and Spices from Mexican Market. Journal of Food Protection 5, 99-103.
Geeta H, Kulkarni PR. 1987. Survey Of the Microbiological Quality Of Whole Black Pepper And Turmeric Powder Sold In Retail Shops In Bombay. Journal of Food Protection 50, 401-403.
ISO 1003. 2025. Spices and condiments — Dried ginger, whole/chopped and ground Zingiber officinale Roscoe- Specifications, 9p.
ISO 15213-1. 2023. Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. Part 1: Enumeration of sulfite-reducing Clostridium spp. by colony-count technique, 21p.
ISO 16649-2. 2001. Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide, 8p.
ISO 1842. 1991. Fruit and vegetable products – Determination of pH, 2p.
ISO 20377. 2018. Dried parsley Petroselinum crispum -Specification, 9p.
ISO 21527-1. 2008. Microbiology of food and animal feeding stuffs- Horizontal method for the enumeration of yeasts and molds Part 1: Colony count technique in products with water activity greater than 0.95, 9p.
ISO 4833-1. 2013. Microbiology of the food chain- Horizontal method for the enumeration of microorganisms Part 1: Colony count at 30 °C by the pour plate technique, 9p.
ISO 6579-1. 2017. Microbiology of the food chain- Horizontal method for the detection, enumeration and serotyping of Salmonella Part 1: Detection of Salmonella spp, 50p.
ISO 6888-1. 2021. Microbiology of the food chain- Horizontal method for the enumeration of coagulase-positive staphylococci Staphylococcus aureus and other species Part 1: Method using Baird-Parker agar medium, 20p.
ISO 712-1. 2024. Cereals and cereal products- Determination of moisture content- Part 1: Reference method, 16p.
ISO 7932. 2004. Microbiology of food and animal feeding stuffs- Horizontal method for the enumeration of presumptive Bacillus cereus- Colony-count technique at 30 degrees C, 13p.
ISO-5559. 1995. Dehydrated onion Allium cepa Linnaeus- Specification, 9p.
Kadariya J, Smith TC, and Thapaliya D. 2014. Staphylococcus aureus and staphylococcal foodborne diseases: an ongoing public health challenge. BioMed Research International, 2014, 1-9. https://doi.org/10.1155/2014/827965.
Koohy-Kamaly-Dehkordy P, Nikoopour H, Siavoshi F, Koushki M, Abadi A. 2013. Microbiological quality of retail spices in Tehran, Iran. Journal of Food Protection 76, 843-848. https://doi.org/10.4315/0362-028X.JFP-12-180.
Kucukoglu S, Hiz G, Karaca H. 2024. Effects of thermal and nonthermal treatments on microorganisms, pyrrolizidine alkaloids and volatile compounds in oregano Origanum vulgare L., Food Chemistry 440. https://doi.org/10.1016/j.foodchem.2023.138235.
Kurzeja E, Stec M, Ramos P, Pilawa B, Pawłowska-Góral K. 2012. The influence of sterilization on free-radical generation, discoloration and the antioxidant properties of certain spice herbs. Italian Journal of Food Science 24, 254-261.
NBF 01-197. 2014. Dried soumbala: specifications 8p.
Ogur S. 2022. Microbiological Quality and Safety of Some Dried Spices Obtained from Markets, Spice Shops and Homes. Food/Feed Science and Technology 65, 1-14. http://dx.doi.org/10.1590/1678-4324-2022220315 .
Ouoba LLI, Diawara B, Amoa-Awua WK, Traore AS, Moller PL. 2004. Genotyping of starter cultures of Bacillus subtilis and Bacillus pumilus for fermentation of African locust bean Parkia biglobosa, to produce soumbala. International Journal of Food Microbiology 90, 197–205.
Paramasivam S, Thangaradjou T, Kannan L. 2007. Effect of natural preservatives on the growth of histamine producing bacteria. Journal of Environmental Biology 28, 271-274.
Parveen S, Das S, Begum A, Sultana N, Hoque MM, Ahmad I. 2014. Microbiological quality assessment of three selected spices in Bangladesh. International food research journal 21, 1327-1330.
Phan A, Mijar S, Harvey C, Biswas D. 2025. Staphylococcus aureus in foodborne diseases and alternative intervention strategies to overcome antibiotic resistance through the use of natural antimicrobials. Microorganisms 13, 1-37. https://doi.org/10.3390/microorganisms13081732.
Schraufstatter E, Bernt H. 1949. Antibacterial action of curcumin and related compounds. Nature 146, 456-457. https://doi.org/10.1038/164456a0.
Schweiggert U, Carle R, Schieber A. 2007. Conventional and alternative processes for spice production—a review. Trends in Food Science and Technology 18, 260–268. https://doi.org/10.1016/j.tifs.2007.01.005.
Tankoano A, Kaboré K, Coulibaly-Diakité M, Somda NS, Somé KI, Rouamba A, Kaboré D, Savadogo A, Sawadaogo-Lingani H. 2025. Nutritious Raw Soumbala Seasoning: An Alternative for Its Valorization and Acceptability. Journal of Advances in Biology Biotechnology 28, 98–111. https://doi.org/10.9734/jabb/2025/v28i11864 .
Tankoano A, Kaboré K, Samandoulougou-Kafando PMJ, Some KI, Coulibaly-Diakité M, Kaboré D, Savadogo A, Sawadaogo-Lingani H. 2024. Characterization of spice ingredients and raw soumbala for the formulation of a highly nutritious seasoning, Cogent Food Agriculture 10, 1-9. https://doi.org/10.1080/23311932.2024.2413387.
Taram I. 2023 Development of a condiment based on onion Allium cepa and spices rich in iron, zinc, and magnesium Master’s thesis, University of Ouaga 1 Professor Joseph Ki Zerbo, 69p.
Thuillier F. and Traoré, A. 2012. Drying tropical fruits for export: the experience of dried mango in Burkina Faso. African Network for Horticulture Development RADHORT, 10p. https://openknowledge.fao.org/handle/20.500.14283/az829f.
Wada NM, Ambi AA, Ibrahim AA, Bello SK, Umar A, James DT. 2021. Antimicrobial Activity of Extracts of Turmeric Curcuma longa and Garlic Aliium sativum Against Selected Bacterial Clinical Isolates. Mediterranean Journal of Infection Microbes and Antimicrobials 10, 1-7. https://doi.org/10.4274/mjima.galenos.2021.2020.6.
Zheng X, Liang S, Wen L, Wang H, Bai Y, Wang Y, Cao L, Li D, Guo J, Chen Y, Wang C, Liu X, Zhang C. 2025. Foodborne outbreak of enterotoxigenic Staphylococcus aureus at a school in Hebei, China. Food Research International 214, 116645. https://doi.org/10.1016/j.foodres.2025.116645.
Pingdwindé Marie Judith Samadoulougou-Kafando, Korotimi Traoré, Crépin Ibingou Dibala, Aboubacar Sidiki Dao, Josias Nikiema, Idrissa Taram, Adama Pare, Inoussa Salambéré, Donatien Kaboré, Charles Parkouda, 2025. Improving the microbiological quality of spices and spice blends using treatments accessible to SMEs/SMIs. Int. J. Biosci., 27(6), 102-113.
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