Phenotypic characterization of bacteria isolated from blood cultures at the Albert Royer National Children’s Hospital (CHNEAR) in 2023
Paper Details
Phenotypic characterization of bacteria isolated from blood cultures at the Albert Royer National Children’s Hospital (CHNEAR) in 2023
Abstract
Septicemia remains a major cause of morbidity and mortality in pediatric populations and poses a significant global public health challenge due to antimicrobial resistance (AMR). Blood culture is the gold standard for diagnosis and provides essential data for guiding empirical antibiotic therapy. In Senegal, limited data are available on the bacteriological profile of bloodstream infections at the Centre Hospitalier National d’Enfants Albert Royer (CHNEAR), the national pediatric referral center. This retrospective descriptive study with analytical components examined bacteria isolated from blood cultures between January 1 and December 31, 2023. Patient and laboratory data were retrieved from registers and a local database (FileMaker Pro Advanced 11.0 v1). Blood cultures were processed using the BACT/ALERT system. Antibiotic susceptibility testing was performed using the Kirby–Bauer method and analyzed with the ADAGIO system. Data were extracted into Microsoft Excel 2013 and analyzed using STATA version 14.0. A total of 1,104 blood culture bottles were analyzed, primarily from the emergency department. The mean patient age was 17.67 years, with a male-to-female ratio of 1.32. The overall positivity rate was 26.81%, yielding 296 bacterial isolates. The predominant organisms were Staphylococcus spp. (43.77%), Klebsiella spp. (13.47%), Pseudomonas spp. (10.77%), and Escherichia spp. (10.10%). Extended-spectrum beta-lactamase (ESBL)-producing strains accounted for 38 isolates, mainly Klebsiella pneumoniae (47.37%), while 40.68% of Staphylococcus aureus isolates were methicillin-resistant (MRSA). These findings highlight moderate but concerning levels of antibiotic resistance. Strengthening local AMR surveillance and implementing targeted interventions aligned with the One Health approach are essential to improving septicemia management and reducing mortality.
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B. Dieye*, A. Diop, A. A. Niang, F. Diallo, M. A. Sonko, F. Thiam, S. S. Mbacke, C. B. Fall, M. Der, A. T. M. Diallo, A. Gaye, A. Dieng, R. Ka, M. L. Dia, 2026. Phenotypic characterization of bacteria isolated from blood cultures at the Albert Royer National Children’s Hospital (CHNEAR) in 2023. Int. J. Microbiol. Mycol., 22(1), 1-11.
Copyright © 2026 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.