In vitro antibacterial effect of Tinospora cordifolia extracts against some selective bacterial pathogens
Paper Details
In vitro antibacterial effect of Tinospora cordifolia extracts against some selective bacterial pathogens
Abstract
The petroleum spirit, methanol, dichloromethane and ethyl acetate extracts of the leaves of Tinospora cordifolia (Menispermaceae) were screened for their anti-microbial activity using disc-diffusion method on nutrient agar medium. This plant was tested against four bacteria; two Gram-positive bacteria (Bacillus subtilis and Sarcina lutea) and two Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae).All the organic solvent extract showed susceptibility against Sarcina lutea, E.coli and Bacillus subtilis whereas Klebsiella pneumoniae showed resistant against all the organic solvent extract. It was found that the antimicrobial activity of the methanol extract (11mm) showed the maximum zone of inhibition against Escherichia coli. On the other hand petroleum spirit extract showed the maximum inhibition against Bacillus subtilis (3mm) respectively. The minimum inhibitory concentration for petroleum spirit and dichloromethane extracts of Tinospora cordifolia were ranged between 32-512µg/mL for tested bacteria. The result of this study demonstrates the potentiality of Tinospora cordifolia as a source of antimicrobials that could be harness for use in the health care delivery process.
Achi OK. 2006. Composition and antibacterial activities of Tetrapleura tetraptera Taub. pod extracts. Research Journal of Microbiology 5, 416 – 422. http://dx.doi.org/103923/jm.2006. 416.422
Egwari LO. 1999. Antibacterial activity of crude extracts of Nauclea latifolia and Eugenia aromatica. West African Journal of Pharmacology and drug Resistance 15, 55 – 59.
Fritsche TR, McDermott PF, Shryock TR, Walker RD. 2007. Agar dilution and disk diffusion susceptibility testing of Campylobacter spp. Journal of Clinical Microbiology, 45, 2758-2759. http://dx.doi.org/10.1128/JCM.00569-07
Gur S, Turgut-Balik D, Gur N. 2006. Antimicrobial activities and some fatty acids of turmeric, ginger root and linseed used in treatment of infectious diseases. World Journal of Agricultural Sciences 2, 439-442.
Jagetia GC, Rao SK. 2006. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma bearing mice. Biological and Pharmaceutical Buletins 29, 460-466. http://dx.doi.org/10.1248/bpb.29460
Kirtikar KR, Basu BD. 1933. Indian medicinal plants 2, 77.
Methew S, Kuttan G. 1999. Immunomodulatory and antitumour activities of Tinospora cordifolia. Fitoterapia 70, 35-43. http://dx.doi.org/10.1016/S0367-326X(98)00017-3
Osadebe PO, Okoye FB, Uzor PF, Nnamani NR, Adiele IE, Obiano NC. 2012. Phytochemical analysis, hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pacific Journal of tropical Medicine 5, 289-293. http://dx.doi.org/10.1016/S1995-7645(12)60041-8
Palombo EA, Semple SJ. 2001. Antibacterial activity of traditional Australian medicinal plants. Journal of Ethnopharmacology 77, 151-157. http://dx.doi.org/10.1016/ S0378-8741(01)00290-2
Penna C, Marino S, Vivot E, Cruanes MC, de D Munoz J, Cruanes J, Ferraro G, Gutkind G, Martino V. 2001. Antimicrobial activity of Argentine plants used in the treatment of infectious diseases. Isolation of active compounds from Sebastiania brasiliensis. Journal of Ethnopharmacology 77, 37-40. http://dx.doi.org/10.1016/S0378-8741(01)00266-5
Stanely P, Prince M, Menon VP. 2000. Hypoglycaemic and other related actions of Tinospora cordifolia roots in alloxan- induced diabetic rats. Journal of Ethnopharma – cology 70, 9-15. http://dx.doi.org/10.1016/S0378-8741(99)00136-1
Sinha K, Mishra NP, Singh J, Khanuja SPS. 2004. Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: A review. Indian Journal of Traditional knowledge 3, 257-270.
Shahidi GA. 2004. Evaluation of antimicrobial properties of Iranian medicinal plants against Micrococcus luteus, Serratiamarceceans, Klebsiella pneumonia and Bordetellabranchoseptica. Asian Journal of Plant Sciences 3, 82-86.
Tschesche R. 1971. Advances in chemistry of Antibiotics substance from higher plant; pharmacognosy and phytochemistry proceeding of the 1st international congress. Verlong, Berlin, Heidelbeg, New York, 274 – 276.
Md. Shamim Hossain, Mst. Touhida khatun1, Md. Mehedi Hassan (2013), In vitro antibacterial effect of Tinospora cordifolia extracts against some selective bacterial pathogens; IJB, V3, N7, July, P156-161
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