Cholinesterase inhibition and antioxidant activity of the stem bark of Abroma augusta: Correlation with phenolic and flavonoid content
Paper Details
Cholinesterase inhibition and antioxidant activity of the stem bark of Abroma augusta: Correlation with phenolic and flavonoid content
Abstract
Abroma augusta is a popular folk medicine in Bangladesh used to treat a variety of diseases. The present study aimed to evaluate the anticholinesterase and antioxidant activity of the plant extract and its four fractions in vitro in order to explore new drugs or alternative treatment for AD. The cholinesterase inhibitory activity was performed by Ellman’s method and the antioxidant activity by DPPH free radical scavenging assay. The methanol extract exhibited inhibition of both the acetyl- and butyryl-cholinesterase enzymes and showed antioxidant properties. When the extract was fractionated, the activities were mostly found in the chloroform fraction. The chloroform fraction showed the IC50 values of 390.53±0.29 and 350.37±0.21 μg/ml for acetyl- and butyryl-cholinesterase, respectively. Importantly the chloroform fraction showed potential antioxidant activity with an IC50 value of 10.25 ± 0.03 μg/ml for DPPH radical scavenging. The fraction was also found to be rich in phenolic content (97.8±0.07 mg GAE/gm of dried extract) and flavonoid content (108.60±0.49 mg CE/g dried extract). A linear correlation was found between phenolic and flavonoid content with acetyl- and butyryl-cholinesterase inhibition and antioxidant activity. The cholinesterase inhibitory and antioxidant activity of the chloroform fraction suggest that it may be useful in the treatment of AD. Further studies in animal model are required to confirm the anti-AD potential of the chloroform fraction.
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K. M Monirul Islam, Tahmida Sharmin, Md. Minhaz Khan Mithun, Simin Shabnam Lopa, Joya Rani*, Md. Aslam Sheikh, Md. Golam Sadik, 2026. Cholinesterase inhibition and antioxidant activity of the stem bark of Abroma augusta: Correlation with phenolic and flavonoid content. Int. J. Biosci., 28(5), 117-124.
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