Assessment of physicochemical parameters and bacteriological quality of pond water of Sylhet agricultural university, Sylhet

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Research Paper 10/09/2026
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Assessment of physicochemical parameters and bacteriological quality of pond water of Sylhet agricultural university, Sylhet

Parsha Shanzana*, Muktarul Jannat Rafa, Rubayet Jahan Nuha
Int. J. Biosci. 29(3), 87-93, September 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

Water quality is indispensable for sustaining aquaculture and safeguarding public health, particularly in Bangladesh, where fisheries play a conclusive role in the national economy. This study aimed to assess the physicochemical and bacteriological quality of pond water from three designated ponds at Sylhet Agricultural University. The primary aim was to evaluate the ponds’ suitability for aquaculture and to assess potential microbial contamination risks. Sterile methods were used to collect water samples and then a multi-parameter water quality meter and standard chemical test kits were used to look at important physicochemical parameters like pH, temperature, dissolved oxygen (DO), salinity, total dissolved solids (TDS), conductivity, ammonia, and nitrate levels. Microbiological analysis was conducted using serial dilution and culture techniques on selective media, succeeded by biochemical tests including IMViC, Triple Sugar Iron (TSI), citrate utilization, catalase, and other standard identification methods. The results showed noticeable variation among the three ponds. The pH values were slightly alkaline, ranging from 8.2 to 8.9, which in some cases exceeded the recommended range for aquaculture. Dissolved oxygen levels varied widely, with one pond showing critically low DO (1.32 mg/L), indicating unsuitable conditions for aquatic organisms. Ammonia was detected in one pond, while nitrate was absent in all samples. Microbial analysis revealed high bacterial loads, particularly in the pond with low oxygen and higher turbidity. Biochemical characterization indicated the presence of potentially pathogenic bacteria such as Escherichia coli, Salmonella spp., and Edwardsiella spp., suggesting contamination from fecal matter and organic waste. Overall, the findings highlight that while the studied ponds at Sylhet Agricultural University provide the basic environment for aquaculture, variations in physicochemical parameters and the presence of pathogenic bacteria pose significant risks to fish health and public safety. The critically low dissolve oxygen and detection of fecal-associated microbes underscore the urgent need for improved pond management and regular monitoring.

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