Probiotic behavior study of four Enterococcus faecium isolated from raw milk in Algeria

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Research Paper 01/01/2020
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Probiotic behavior study of four Enterococcus faecium isolated from raw milk in Algeria

Loumani Akil, Larbi Ahmed Amine, Bekada Ahmed Mohamed Ali, Bensoltane Ahmed
Int. J. Biosci.16( 1), 301-307, January 2020.
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Abstract

Lactic acid bacteria (LAB) were isolated from spontaneously fermented raw goat milk collected from Relizane, Algeria, and assessed for their probiotic properties. Four acid-tolerant strains were selected. By using 16S rDNA sequencing, these isolates were confirmed to be Enterococcus faecium. The strains exhibited good survivability in a highly acidic environment at pH 2 and in bile salts at concentrations of 0.3% and 2%. The strains were susceptible to antibiotics except colistin and were found to inhibit the growth of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Salmonella Typhi. The strains were nonhemolytic and showed weak production of exopolysaccharides (EPSs). The study confirmed that all the identified strains have in vitro probiotic properties.

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Abushelaibi A, Al-Mahadin S, El-Tarabily K, 2017. Characterization of potential probiotic lactic acid bacteria isolated from camel milk. LWT – Food Science and Technology 79:. DOI: 10.1016/ j.lwt. 2017.01.041

Ahmadova A, Todorov SD, Choiset Y, 2013 Evaluation of antimicrobial activity, probiotic properties and safety of wild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal cheese. Food Control 30, 631-641.

Angmo K, Kumari A, Bhalla TC, others. 2016. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT-food Science and Technology 66, 428-435.

Banwo K, Sanni A, Tan H. 2013. Technological properties and probiotic potential of Enterococcus faecium strains isolated from cow milk. Journal of applied microbiology 114, 229-241.

Barbosa J, Borges S, Teixeira P. 2014. Selection of potential probiotic Enterococcus faecium isolated from Portuguese fermented food. International journal of food microbiology 191, 144-148.

Belgacem Z Ben, Abriouel H, Omar N Ben, 2010. Antimicrobial activity, safety aspects, and some technological properties of bacteriocinogenic Enterococcus faecium from artisanal Tunisian fermented meat. Food Control 21, 462-470.

Chang LIU, Zhang ZY, Ke D, 2009. Antibiotic resistance of probiotic strains of lactic acid bacteria isolated from marketed foods and drugs. Biomedical and Environmental Sciences 22, 401-412.

de Almeida Júnior WLG, da Silva Ferrari Í, de Souza JV, 2015. Characterization and evaluation of lactic acid bacteria isolated from goat milk. Food control 53, 96-103.

Malakar B, Das AJ, Deka SC. 2017. In vitro probiotic potential of Enterococcus species isolated from tungrymbai, a fermented soybean product of Meghalaya, India. Acta Alimentaria 46, 297-304.

Nueno-Palop C, Narbad A. 2011. Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut. International journal of food microbiology 145, 390-394.

on Food Additives, Meeting JFEC, Organization WH. 2002. Evaluation of certain mycotoxins in food: fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organization.

Pieniz S, Andreazza R, Anghinoni T, 2014. Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s. Food Control 37, 251-256. DOI: 10.1016/j.foodcont.2013.09.055

Rehaiem A, Ben Z, Reza M, 2014. Assessment of potential probiotic properties and multiple bacteriocin encoding-genes of the technological performing strain Enterococcus faeciummmRA. Food Control 37, 343-350. DOI: 10.1016/j.foodcont.2013.09.044

Shehatamg, El Sohaimy SA, El-Sahn MA, Youssefmm. 2016. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Annals of Agricultural Sciences 61, 65-75.

Wayne PA. 2010. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100-S20