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An endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier with a dual activity against carboxymethylcellulose and xylan

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Research Paper 01/02/2012
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An endo-beta-D-glycosidase from salivary glands of Macrotermes subhyalinus little soldier with a dual activity against carboxymethylcellulose and xylan

Fagbohoun Jean Bedel, Ahi Amedée Pascal, Karamoko Yahaya, Dabonné Soumaila, Kouadio Eugène Jean Parfait and Kouamé Lucien Patrice
Int. J. Biosci.2( 2), 1-10, February 2012.
Certificate: IJB 2012 [Generate Certificate]
Key: Bifunctional enzymeCarboxymethylcellulaseEndo-beta-D-glycosidase

Abstract

This study highlights an endo-beta-D-glycosidase from little soldier of Macrotermes subhyalinus purified by anion exchange, cation exchange and hydrophobic interaction chromatography. The only substrates that were hydrolyzed by the purified enzyme were xylans and carboxyméthylcellulose. The enzyme showed a single protein band and its relative molecular weight was estimated to be 215.45±5.63 kDa. The specific activities towards carboxymethylcellulose and xylan from Birchwood were respectively 9.32±3.78 and 8.59±2.54 U/ mg of protein. The purified enzyme showed an optimum pH of 4.6 for cellulase activity an 5.0 for xylanase activity in acetate buffer. The optimum temperature of the enzyme with CMC and xylan from Birchwood hydrolysis were found to be 60 and 55 °C respectively.

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Arakawa GH, Watanabe H, Yamasaki H and G. Tokuda G. 2009. Purification and molecular cloning of xylanases from the wood-feeding terminte, Coptotermes formosanus Shiraki. Biosci. Biothecnol. Biochem. 73 (3), 710-718.

Barr BK, Hsieh YL, Ganem B, Wilson DB. 1996. Identification of two functionally different classes of exocellulases,” Biochemistry 35(2), 586-592.

Binaté S, N’dri D, Toka M, Kouamé LP. 2008. Purification and characterization of two beta-glucosidases from termite workers Macrotermes bellicosus (Termitidae: Macrotermitinae). Journal of Applied Bioscience 10, 461-470.

Bléi SH, Soro RY, Dabonné S, Kouamé LP. 2010. A novel polysaccharidase with endo-beta-D-xylanase and endo-beta-D-glucanase activities in the gut of the major soldier of the termite Macrotermes subhyalinus. Journal of Animal & Plant Sciences 8(1), I, 912- 926.

Blum H, Beier H, Goss B. 1987. Implored silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93- 99.

Chadha BS, Jaswinder K, Rubinder K, Saini HS, Singh S. 1999. Xylanase production by Thermomyces lanuginosus wild and mutant strains. World J. Microbiol. Biotechnol. 15, 195-198.

Faulet MB, Niamké S, Gonnety TJ, Kouamé LP. 2006a. Purification and biochemical characteristics of a new strictly specific endoxylanase from termite Macrotermes subhyalinus workers. Bull. Insectology 59, 17-26.

Gaffney M, Carberryb S, Doyleb S, Murphy R. 2009. Purification and characterization of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris Enzyme and Microbial Technology 45,3 48–354.

Juhász, T, Szengyel Z, Réczey K, Siika-Aho M, Viikari L. 2005. Characterisation of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. Process Biochem. 40, 3519– 25.

Kouamé LP, Kouamé AF, Niamké SL, Faulet MB, Kamenan A. 2005. Biochemical and catalytic properties of two beta-glycosidases purified from workers of the termite Macrotermes subhyalinus (Isoptera: Termitidae). International Journal of Tropical Insect 25, 103-113.

Lama L, Calandrelli V, Gambacorta A, Nicolaus B. 2004. Purification and characterization of thermostable xylanase and β-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. Research in Microbiology 155, 283–289.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.

Nakashima K, Watanabe H, Saitoh H, Tokuda G, Azuma J. 2002. Dual cellulose-digestiing system of the wood-feeding termite Coptotermes formosanus Shiraki. Insect Biochem. Mol. Bio. 32, 777-784.

Ninawe S, Kapoor M, Kuhad RC. 2008. Purification and characterization of extracellular  xylanase from Streptomyces cyaneus SN32. Bioresource Technology 99, 1252-1258.

Pérez J, Muñoz-Dorado J, De-la-Rubia T, Martínez J. 2002. Biodegradation and biological treatments of cellulose, hemicellulose and lignin : an overview. Inter. Microbiol. 5, 53-63.

Pérez-Avalos O, Sa´nchez-Herrera LM, Salgado, Ponce-Noyola T. 2008. A bifunctional endoglucanase/endoxylanase from Cellulomonas flavigena with potential use in industrial processes at different pH. Current Microbiology 57, 39-44.

Reverbel-Leroy C, Pages S, Belaich A, Belaich JP, Tardif C. 1997. The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome : purification and characterization of the recombinant form,” Journal of Bacteriology 179 (1), 46-52.

Rouland C, Civas A, Renoux J. Petek F. 1988a. Purification and properties of cellulases from the termite Macrotermes mulleri (Termitidae: Macrotermitinae) and its symbiotic fungus. Termitomyces sp. Comparative Biochemistry and Physiology 91B, 449-458.

Rouland C, Civas A, Renoux J, Petek F. 1988b. Synergistic activities of the enzymes involved in cellulose degradation, purified from Macrotermes mulleri fungus Termitomyces sp. Comparative Biochemistry and Physiology 91B, 459-465.

Séa TB, Saki SJ, Coulybaly AF, Yeboua AF, Diopoh KJ. 2006. Extraction, purification et caractérisation de deux cellulases du termite Macrotermes subhyalinus (Termitidae). Agronomie Africaine 10, 57-65.

Tokuda G, Lo N, Watanabe H, Matsumoto T, Noda H. 1999. Metazoan cellulase genes from termites: intron/ exon structures and sites of expression . Biochim. Biophys . Acta 1447, 146-159.

Tuomela M, Vikman M, Hatakka A, Itavaara M. 2000. Biodegradation of lignin in a compost environment : a review. Bioresource Technology 72, 169-183.

Veivers PC, Muhlemann R, Slaytor M, Leuthold RH, Bignell DE. 1991. Digestion, diet and polyethism in two fungus-growing termites: Macrotermes subhyalinus Rambur and M. michaelseni Sjostedt. Journal of Insect Physiology 37, 675-682.

Watanabe H, Noda H, Tokuda G, Lo N. 1998. A cellulase gene of termite origin. Nature 394, 330-331.

Watanabe H, Nakamura M, Tokuda G, Yamaoka I, Scrivener AM, Noda H. 1997. Site of secretion and properties of endogenous endo- β -1,4-glucanase components from Reticulitermes speratus (Kolbe), a Japanese subterranean termite. Insect Biochemistry and Molecular Biology 27, 305–313.

Zimmerman P, Greenberg J, Wandiga S, Crutzen P. 1982. Termites : a potentially large source of atmospheric methane, carbon dioxide and molecular hydrogen. Science 218, 563-565.

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