A comparative analysis of the secondary metabolites, polyphenol contents and antioxidant potential of ethanolic, hydro-ethanolic and aqueous extracts of Annona muricata Linn leaves in Abomey-Calavi, Benin
Paper Details
A comparative analysis of the secondary metabolites, polyphenol contents and antioxidant potential of ethanolic, hydro-ethanolic and aqueous extracts of Annona muricata Linn leaves in Abomey-Calavi, Benin
Abstract
Annona muricata Linn commonly called corossol in BENIN is a plant of the family of Annonacceae known throughout the world through its virtues in traditional medicine. This study aimed to compare the phytochemical composition and the antioxidant activity between ethanolic, hydro-ethanolic, and aqueous extracts of Annona muricata leaves collected in Abomey-Calavi, Benin. Qualitative and quantitative phytochemical tests were used to detect the presence of bioactive molecules. Antioxidant activity was performed using the DPPH method and the FRAP reducing power method. The phytochemical screening showed the presence of flavonoids, tannins (catechic and gallic), alkaloid, anthocyanin, leuco anthocyanins, triterpenoids, steroids, mucilage, reducing compounds and coumarins in all three extracts. The ethanolic extract showed a higher antioxidant potential (IC50= 0.01mg/ml) than the hydro-ethanolic (IC50= 0.013mg/ml) and aqueous extracts (IC50= 0.014mg/ml) by the DDPH method. The ethanolic extract showed a higher reducing power than the aqueous extract and the hydro-ethanolic by the FRAP test. This confirms the agreement in the results. However, the ethanolic extract of Annona muricata is less active than ascorbic acid, which is a reference antioxidant.
Abdul Wahab SM, Jantan I, Haque Md A, Arshad L. 2018. Exploring the Leaves of Annona muricata L. as a Source of Potential Anti-inflammatory and Anticancer Agents. Frontiers in Pharmacology 9. https://doi.org/10.3389/fphar.201
Adewole S, Caxton-Martins E. 2009. Morphological changes and hypoglycemic effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of Biomedical Research 9(3). https://doi.org/10.4314/ajbr.v9i3.48903
Ahmed D, Khan MM, Saeed R. 2015. Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants 4(2), 394. https://doi.org/10.3390/antiox4020394
Ahoton D, Assogba MF, Ladekan ECY, Davo A, Moudachirou M, Gbenou JD. 2019. Phenolic compounds’ dosage and antioxidant activities of aqueous and ethanolic extracts of Lophira lanceolata’s Van Tiegh. Ex Keay (Ochnaceae) leaves. International Journal of Phytopharmacy.
Ana C, Montalvo-Gónzalez E, Yahia E, Obledo-Vázquez E. 2016. Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry 11. https://doi.org/10.1016/j.arabjc16.01
Assogba Fidèle M, Aderomou chouaibou, Agbodjogbe Wilfrid, Moudachirou Mansourou, Joachim D Gbenou. 2015. Evaluation of diuretic properties from Elaeis guineensis Jacq. (Arecacea) leaves aqueous extract in wistar rat [Journal of Chemical and Pharmaceutical research 7(3), 2457-2462,]. https://bec.uac.bj/publication/5631/Article
Benzie IFF, Strain JJ. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power” : The FRAP Assay. Analytical Biochemistry 239(1), 70‑76. https://doi.org/10.1006
Coria-Téllez AV, Montalvo-Gónzalez E, Yahia EM, Obledo-Vázquez EN. 2018. Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry 11(5), 662‑691. https:// doi.org/10.1016/j.arabjc.2016.01.004
Ezealisiji KM, Belema TE. 2017. Comparative evaluation of the phenolic and antioxidant properties of the leaves, root, stem bark, and root bark of Annona muricata (Annonaceae). Journal of Pharmacognosy and Phytochemistry 6(2), 274‑278.
Florence NT, Benoit MZ, Jonas K, Alexandra T, Désiré DDP, Pierre K, Théophile D. 2014. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 151(2), 784‑790. https:// doi.org/ 10.1016/j.jep.2013.09.021
Gavamukulya Y, Abou-Elella F, Wamunyokoli F, AEl-Shemy H. 2014. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific Journal of Tropical Medicine 7S1, S355-363.
George VC, Kumar DRN, Suresh PK, Kumar RA. 2015. Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. Journal of Food Science and Technology 52(4), 2328‑2335.
Hansra D, Silva O, Mehta A, AhnE. 2014. Patient with Metastatic Breast Cancer Achieves Stable Disease for 5 Years on Graviola and Xeloda after Progressing on Multiple Lines of Therapy. Advances in Breast Cancer Research 03, 84‑87.
Hasmila I, Natsir H, Soekamto NH. 2019. Phytochemical analysis and antioxidant activity of soursop leaf extract (Annona muricata Linn.). Journal of Physics: Conference Series 1341, 032027.
Heimler D, Vignolini P, Dini MG, Vincieri FF, Romani A. 2006. Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chemistry. https://agris.fao.org /agris-search/search.do?recordID=US201300738619
Houghton P, Raman A. 1998. Laboratory handbook for the fractionation of natural extracts. https://www.google.fr/search?source=hp&ei=BhrSXpyDO6eMlwTGubbQAQ&q=Houghton+P
Ishuwa M, Elkanah O, Wahedi J. 2016. Grain Protectant Potential of Annona muricata and A. senegalensis against Cowpea Seed Bruchid Callosobruchus maculatus Fab. (Coleoptera : Bruchidae). Asian Research Journal of Agriculture 1(1), 1‑7. https://doi.org/10.9734/ARJA/2016/ 2605
Iyanda-Joel WO, Ajetunmobi OB, Chinedu SN, Iweala EEJ, Adegbite OS. 2019. Phytochemical, Antioxidant and Mitochondrial Permeability Transition Studies on Fruit-Skin Ethanol Extract of Annona muricata [Research Article]. Journal of Toxicology; Hindawi. https://doi.org/10.1155/ 2019
JJ, CS, EL, Jm V. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry 83(4), 547‑550. https://doi.org/ 10.1016/ s0308-8146 (03)00151-1
Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. Journal of Agricultural and Food Chemistry 52(11), 3389‑3393. https://doi.org/10.1021/jf049899k
Justino AB, Miranda NC, Franco RR, Martins MM, Silva NM da, Espindola FS. 2018. Annona muricata Linn. Leaf as a source of antioxidant compounds with in vitro antidiabetic and inhibitory potential against α-amylase, α-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation. Biomedicine & Pharmacotherapy 100, 83‑92. https://doi.org/10.1016/j.biopha.2018.01.172
Kim DO Ok C, Yj K, Hy M, Cy L. 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry 51(22), 6509‑6515. https://doi.org/ 10.1021 /jf0343074
Koné K, Yaya S, Sorho S. 2017. Détermination des paramètres influençant le rendement d’extraction hydro-alcoolique des métabolites secondaires de Alchornea cordifolia (Euphorbiaceae) et Tridax procumbens Linn (Asteraceae) Détermination des paramètres influençant le rendement d’extraction hydro-alcoolique des métabolites secondaires de Alchornea cordifolia (Euphorbiaceae) et Tridax procumbens Linn (Asteraceae).
Leatemia JA, Isman MB. 2004. Insecticidal activity of crude seed extracts of Annona spp., Lansium domesticum and Sandoricum koetjape against lepidopteran larvae. Phytoparasitica 32(1), 30‑37.
Lhuillier A. 2007. Contribution à l’étude phytochimique de quatre plantes malgaches : Agauria salicifolia Hook.f ex Oliver, Agauria polyphylla Baker (Ericaceae), Tambourissa trichophylla Baker (Monimiaceae) et Embelia concinna Baker (Myrsinaceae). [Phd].
Nascimento GGF, Locatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology 31(4), 247‑256.
Nga EN, Nchinda G, Mahi LL, Figuei PB, Mvondo JM, Sagnia B, Adiogo D. 2018. Phytochemical Screening and Evaluation of Antioxidant Power of Hydro-Ethanolic and Aqueous Leaves Extracts of Annona muricata Linn (Soursop). Health Sciences And Disease, 20(1), Article 1.
Nwokocha CR, Owu DU, Gordon A, Thaxter K, McCalla G, Ozolua RI, Young L. 2012. Possible mechanisms of action of the hypotensive effect of Annona muricata (soursop) in normotensive Sprague–Dawley rats. Pharmaceutical Biology 50(11), 1436‑1441.
Ojezele O, Ojezele M, Adeosun A. 2016. Comparative Phytochemistry and Antioxidant Activities of Water and Ethanol Extract of Annona muricata Linn Leaf, Seed and Fruit. Advances in Biological Research 10, 230‑235. https://doi.org/ 10.5829/idosi.abr.2016.10.4.10514
Onyegeme-Okerenta BMA, Amadi B, O Ezeonyilimba V. 2018. The Ameliorating Potential of Annona muricata on Sodium Fluoride-induced Toxicity on Liver and Kidney of Male Wistar Rats. Journal of Complementary and Alternative Medical Research 6(1), 1‑17. https://doi.org/10.9734/JOCAR
Orak HH, Bahrisefit IS, Sabudak T. 2019. Antioxidant Activity of Extracts of Soursop (Annona muricata L.) Leaves, Fruit Pulps, Peels, and Seeds. Polish Journal of Food and Nutrition Sciences 69(4), 359‑366. https://doi.org/10.31883/pjfns/112654
Oyaizu M. 1986. Studies on products of browning reaction—Antioxidative activities of products of browning reaction prepared from glucosamine. Eiyogaku zasshi= Japanese journal of nutrition. https://agris.fao.org/agris-search/search.do? record ID =US201302009163
Padma P, Chansouria JPN, Khosa RL. 1999. Hepatoprotective activity of Annona muricata Linn. And Polyalthia cerasoides bedd. Ancient Science of Life 19(1‑2), 7‑10.
Parakash D, Brahma UG, Singh N, Singh HB. 2007. Antioxidant and free radicalscavenging activities of seeds and agri-wostes of some varieties of soybean (Glycine max) 104, 783-790.
Pieme CA, Kumar SG, Dongmo MS, Moukette BM, Boyoum FF, Ngogang JY, Saxena AK. 2014. Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells. BMC Complementary and Alternative Medicine 14. https://doi.org/10.1186/ 1472-6882-14-516
Saleem Dr U 2017. Pharmacological Screening of Annona muricata : A Review. Asian Journal of agriculture and biology 5, 38‑46.
Sinsin B, Owolabi L. 2001. Monographie nationale de la diversité biologique. (p. 41) [Rapport de synthèse. MEHU/PNUD].
Vijayameena C, Subhashini G, Loganayagi M, Balasubramanian R. 2013. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. International Journal for Current Microbiology and Applied Sciences 2, 1‑8.
WHO. 2013. Enhancing the Role of Traditional Medicine in Health Systems: A Strategy for the African Region (p. 13). WHO: Regional Committee For Africa.
Widyastuti D, Rahayu P. 2017. Antioxidant Capacity Comparison of Ethanolic Extract of Soursop (Annona muricata Linn.) Leaves and Seeds as Cancer Prevention Candidate. Biology, Medicine, & Natural Product Chemistry 6, 1. https://doi.org/ 10.14421/ biomedich.2017.61.1-4
Yajid A, Rahman H, Wong M, Wan Zain W. 2018. Potential Benefits of Annona muricata in Combating Cancer : A Review. Malaysian Journal of Medical Sciences 25, 5‑15. https://doi.org/10.21315 /mjms2018.25.1.2
Appoline Isabelle A Gbonsou, Mahoudo Fidèle Assogba, Lysette DC Kinsou, Djidénou Ahoton, Mahudjro Yovo, Joachim D Gbenou, Nicolas Atrevi, Patrick A Edorh (2021), A comparative analysis of the secondary metabolites, polyphenol contents and antioxidant potential of ethanolic, hydro-ethanolic and aqueous extracts of Annona muricata Linn leaves in Abomey-Calavi, Benin; JBES, V18, N1, January, P1-10
https://innspub.net/a-comparative-analysis-of-the-secondary-metabolites-polyphenol-contents-and-antioxidant-potential-of-ethanolic-hydro-ethanolic-and-aqueous-extracts-of-annona-muricata-linn-leaves-in-abomey-calavi-b/
Copyright © 2021
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0