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A comparative analysis on mitotic interphase and prophase among twelve varieties of Brassica L. from Bangladesh: Brassicaceae

Research Paper | October 1, 2020

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Susmita Saha, Kazi Nahida Begum

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Int. J. Biosci.17( 4), 73-82, October 2020

DOI: http://dx.doi.org/10.12692/ijb/17.4.73-82


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Brassica L. is an agronomical and economical important crop belonging to Brassicaceae family. The research was conducted to interrelate interphase nuclei and prophase chromosome of Brassica L. varieties. In the current analysis, the nature of interphase nuclei and prophase chromosome of twelve BARI varieties of Brassica L. was investigated based on orcein-staining properties. In the interphase nuclei only ‘Diffuse type’ and ‘Simple Chromocenter type’ were found in Tori-7, Dawlat, BS-11, BS-14 and SS-75, Rai-5, BS-6, BS-7, BS-8, BS-10, BS-12, BS-15, respectively. Considering prophase chromosome among these studied twelve varieties, ‘Continuous type’ showed in Dawlat, Rai-5, BS-7, Bs-8, BS-11, BS-14 while BS-6, BS-10, BS-12 and BS-15 observed ‘Interstitial type’ and ‘Gradient type’ showed in Tori-7, and SS-75. Therefore, the orcein staining property of interphase nuclei and prophase chromosome can provide essential information as cytological implement to discriminate the twelve analyzed Brassica L. varieties.


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A comparative analysis on mitotic interphase and prophase among twelve varieties of Brassica L. from Bangladesh: Brassicaceae

Akbar F, Begum KN. 2020. A comparative anatomical investigation of three taxa of Brassica L. from Bangladesh. Bangladesh Journal of Plant Taxonomy 27(1), 15‒26. https://doi.org/10.3329/bjpt.v27i1.47566

Begum KN, Alam Sk S. 2016. Karyomorphological analysis with differential staining of nine Cicer Arietinum L. Varieties. Bangladesh Journal of Botany 45(2), 327-334.

Chen XJ, Chen ZJ, Du GX, Zhang MF. 2001. Electrophoretic comparative study on polypeptides of chloroplast from cytoplasmic male sterile lines and their maintainer lines on tuber mustards. Journal of Zhejiang University 27, 88-95.

Cheng BF, Heneen WK, Chen BY. 1995. Mitotic karyotypes of Brassica campestris and Brassica alboglabra and identification of the B. alboglabra in addition line. Genome 38, 313-319. https://doi.org/10.1139/g95-039

Downey RK, Robbelen G. 1989. Brassica species: oil crops of the world, their breeding and utilization. New York, USA. McGraw Hill Publishing Co., 339-374.

Du W, Chen QX, Mo H. 1993. Study on somatic chromosome karyotypes of Xinjiang wild rape, B. campestris, B. nigra and B. juncea. Journal of August 1st Agricultural College 16(4), 26-31.

Fang P, Chen FB, Yao QL. 2014. Karyotype analysis on three types of leaf mustard (Brassica juncea). Journal of Changjiang Vegetables 12, 13-17. https://doi.org/10.1139/g95-039

Fukui K, Nagayama S, Ohmido N, Yoshiaki H, Yamabi M. 1998. Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45S rDNA loci on the identified chromosomes. Theoretical and Applied Genetics 96, 325-330.

Hu XM, Chen ZJ, Wang BL. 2001.Comparison on leaf ultrastructure in cytoplasmic male sterile line for tuber mustard (Brassica juncea var. tumida). Journal of Zhejiang University 27, 535-540.

Jahan N, Bhuiyan SR, Talukder MZA, Alam MA, Parvin M. 2013. Genetic diversity analysis in Brassica rapa using morphological characters. Bangladesh Journal of Agricultural Research 38(1), 11–18. https://doi.org/10.3329/bjar.v38i1.15185

Khan MR, Rashid H, Quraishi A. 2002. Effect of various growth regulators on callus formation and regeneration in Brassica napus cv. Oscar. Pakistan Journal of Biological Science 5, 693-695. https://scialert.net/abstract/?doi=pjbs.2002.693.695

Kulak S, Hasterok R, Maluszynska J. 2002. Karyotyping of Brassica amphidiploids using 5S and 25S rDNA as chromosome markers. Hereditas 136, 144-150. https://doi.org/10.1034/j.1601-5223.2002.1360209.x

Liu YH, Leng R, Zhang ZR. 2007. Study on genetic divergence of quantitative characters in tunloros stem mustard (Brassica juncea var. tumida Tsen and Lee). Chinese Agricultural Science Bulletin 23, 328-331.

Luciano FB, Holley RA. 2009.  Enzymatic inhibition by Allyl-isothiocyanate and factors affecting its antimicrobial action against Escherichia coli O157:H7. International Journal of Food Microbiology 131(2-3), 240-245. https://doi.org/10.1016/j.ijfoodmicro.2009.03.005

Mollika SR, Sarkar RH, Hoque MI. 2011. In vitro plant regeneration in Brassica spp. Plant Tissue Culture and Biotechology 21(2), 127-134. https://doi.org/10.3329/ptcb.v21i2.10235

Nagahara U. 1935. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japanese Journal of Botany 7, 389-452.

Olin-Fatih M. 1994. A new method for differential staining of Brassica Metaphase chromosomes and karyotypes of B. campestris, B. oleracea and B. napus. Hereditas 120, 253-259. https://doi.org/10.1111/j.1601-5223.1994.00253.x

Paul M, Islam T, Hoque MI, Sarker RH. 2020. Analysis of genetic diversity in oilseed Brassica germplasm through ISSR markers and isozyme profiling. Bangladesh Journal of Botany 49(1), 147-158.

Rakow G. 2004. Species origin and economic importance of Brassica. In: Biotechnology in Agriculture and Forestry, Ed. E. C. Pua and C. J. Douglas, Berlin: Springer, 3–11.

Raymer PL. 2002. Canola: an emerging oilseed crop, in trends in new crops and new uses. In: Janick J & Whipkey A (eds). American Society for Horticultural Science, Vol. I. Alexandria, USA, ASHS Press, 122–126.

Ropiquet A, Gerbault-Seureau M, Deuve JL, Gilbert C, Pagacova E, Chai N, Rubes J, Hassanin A. 2008. Chromosomal evolution in the subtribe Bovina (Mammalia, Bovidae): The karyotype of the Cambodian banteng (Bos javanicus birmanicus) suggested that Robertsonian translocation are related to interspecific hybridization. Chromosome Research 16, 1107-1118. https://doi.org/10.1007/s10577-008-1262-2

Snowdon RJ. 2007. Cytogenetics and genome analysis in Brassica crops. Chromosome Research 15, 85-95. https://doi.org/10.1007/s10577-006-1105-y

Sultana SS, Alam Sk S. 2016. Karyomorphology of eleven varieties of Gossypium hirsutum L. Bangladesh Journal of Botany 45(1), 151-159.

Sun B, Xia X, Tian Y, Zhang F, Tang H. 2018. Karyotype analysis of Brassica napus cv. huayou no. 2. Advances in Biological Sciences Research 5, 78-81.

Takamine N. 1916. Über die ruhenden und die präsynaptischen. Phasen der Reduktionsteilung. Botanical Magazine 30, 293–303.

Tanaka R. 1971. Types of resting nuclei in Orchidaceae. Botanical Magazine Tokyo 84, 118-1220. https://doi.org/10.15281/jplantres1887.84.118

Wachsman MB, Ramirez JA, Galagovsky LR. 2012. Antiviral activity of Brassionsteroids derivatives against measles virus in cell cultures. Antiviral Chemical and Chemotherapy 13(1), 61–66. https://doi.org/10.1177/095632020201300105

Xiao CG, Guo XH, Han HB, Peng HJ. 2004. Identification of the pathogen for club root on diseased stem mustard in Fuling, Chongqing and its major characteristics. Journal of Southwest Agricultural University 24, 539-541.

Zhang MF. 1996. Tsatsai vegetable growth and flowering response to Paclobutrazol. Acta Agriculturae Zhejiangensis 8, 110-112.

Zhang S, Chiang CY, Xie YF, Park SJ, Lu Y, Hu JW, Dostrovsky JO, Sessle BJ. 2006. Central sensitization in thalamic nociceptive neurons included by mustard oil application to rat molar tooth pulp. Neuroscience 142(3), 833-842.