A comparative study of total phenolics, antioxidant activity and anthocyanins of three Algerian dates varieties

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Research Paper 01/12/2017
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A comparative study of total phenolics, antioxidant activity and anthocyanins of three Algerian dates varieties

Kheira Zerrouki, Ali Riazi, Jacqueline Destain, Philippe Thonart
Int. J. Biosci.11( 6), 16-26, December 2017.
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Abstract

The objective of this study was to compare phenolic profile of three varieties of Algerian dates, and the use for the first time of the oxygen radical absorbance capacity (ORAC-FL) to determine antioxiadant capacity of dates. Total phenolics using Folin-Ciocalteu reagent and the anthocyanins by the pH differential method in three varieties of dates (DgletNour, Ajwa and Ghars). The results obtained were expressed as means of three replicate ± standard deviation (n = 3) on a fresh weight basis. Total polyphenolics values were about 97.16 ±1.25 and 245.32 ± 13.12 and 246 mg/100 g Ferrulic Acid Equivalent (FAE) for Deglet-Nour variety (water/aceton extraction) respectively. In the same order of solvents used. The varieties (Ghars and Ajwa) have presented total phenolics concentrations: (73.81±0.81; 86.63 and 64.77±0.42; 81.11) mg/100 g FAE. These dates’ varieties have shown a good result of total anthocyanins (0.91; 0.75 and 0.56 mg equivalent cyanidine3-glucoside /100g for the varieties Deglet-Nour, Ajwa and Ghars in the same order. ORAC-FL have presented a high results compared by other methods ((DPPH, ABTS, FRAP and TEAC). High values were registred for Deglet-Nour (4844 and 4818.67 ±32.14) µ moles equivalent trolox/100 g and 3891µ moles for Ajwa. Only 2648 µ moles equivalent trolox /100 g were registered for Ghars variety. The temperature effect on pH and total phenolics content was equally tested in this study. In this work, we have added a new conclusion about the high nutrition value of Deglet-Nour variety.

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