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A dose-dependent study of carbon metabolism under Pb stress in the cyanobacterium Nostoc muscorum Meg 1

Research Paper | September 1, 2020

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Balakyntiewshisha L. Kynshi, Rabbul Ibne A. Ahad, Meguovilie Sachu, Luxemburgh Hynniewta, Tridip Phukan, Mayashree B. Syiem

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Int. J. Biosci.17( 3), 241-253, September 2020

DOI: http://dx.doi.org/10.12692/ijb/17.3.241-253


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Lead (Pb) is one of the most abundant heavy metals and its toxic effects cause environmental and health problems. Pb occurs naturally in the environment. However, most lead concentrations that are found in the environment are a result of human activities including burning fossil fuels, mining and manufacturing. In the present study, we checked into the effects of Pb on growth, pigments, PSII activity, carbohydrate and biomass production in the cyanobacterium Nostoc muscorum Meg 1 using different concentrations ranging from 10 ppm to 80 ppm. Pb at lower concentration (10-40 ppm) enhanced the growth by increasing photosynthetic pigments such as chlorophyll a, phycobiliproteins and carotenoids; by increasing concentration of D1 protein that hosts the PSII complex of photosynthetic electron transport chain and by positively modulating the concentration of RuBisCO, the key enzyme for CO2 fixation. Conversely, a higher concentration of Pb (60-80 ppm) adversely affected all parameters studied bringing down the organism’s growth and biomass production. Augmentation of various components of carbon fixation at a lower concentration in this organism indicates that Pb at lower doses was not considered toxic but it could lead to eutrophication of lakes, rivers and water reservoirs rendering them unusable for human and animal consumption.


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A dose-dependent study of carbon metabolism under Pb stress in the cyanobacterium Nostoc muscorum Meg 1

Adir N. 2005. Photo-protection in cyanobacteria: the orange carotenoid protein (OCP) related on photochemical quenching mechanism. Photosynthesis Research 85, 1-15.

Agarwal C, Sen S, Chatterjee A, Rai S, Yadav S, Singh S, Rai LC. 2015. Signal perception and mechanism of salt toxicity/ tolerance in photosynthetic organisms: Cyanobacteria to plants. In: Tripathi BN, Müller M, Ed. Stress Responses in Plants. Springer International Publishing Switzerland., p. 79-113.

Ahad RIA, Goswami S, Syiem MB. 2017. Biosorption and equilibrium isotherms study of cadmium removal by Nostoc muscorum Meg 1: morphological, physiological and biochemical alterations. 3 Biotech 7, 104.

Ahad RIA, Syiem MB. 2018. Copper and cadmium induced toxicity on the cyanobacterium Nostoc muscorum Meg 1: a comparative study. Eurasian Journal of Biosciences 12, 333-345.

Ahad RIA, Syiem MB. 2019. Influence of calcium on cadmium uptake and toxicity to the cyanobacterium Nostoc muscorum Meg 1. Biotechnology Research and Innovation 3, 231-241.

Akbari M, Hallajisani A, Keshtkar AR, Shahbeig H, Ghorbanian SA. 2015. Equilibrium and kinetic study and modelling of Cu(II) and Co(II) synergistic biosorption from Cu(II)-Co(II) single and binary mixtures on brown algae C. indica. Journal of Environmental Chemistry & Engineering 3, 140-149.

Arif TJ, Mudsser A, Kehkashan S, Arif A, Inho C, QaziMohd RH. 2015. Heavy metals and human health: Mechanistic insight into toxicity and counter defence system of antioxidants. International Journal of Molecular Science 16, 29592-29630.

Arunakumara KKIU, Xuecheng Z, Song X. 2007. Comparative study on bioaccumulation of lead and cadmium by the cyanobacterium Synechocystis sp. PCC 6803 under laboratory conditions. Ciencias Marinas 33, 271-280.

ATSDR. 2007. Toxicological profile for lead. Agency for Toxic Substances and Disease Registry (ATSDR). U.S. Department of Health and Human Services- Public Health Service.

Ayansina SA, Olubukola OB. 2017. A new strategy for heavy metal polluted environment: A review of microbial biosorbents. International Journal of Environment Research & Public Health 14, 94.

Ayya Raju M. 2016. Impact of heavy metals poisoning on cyanobacterial photosynthesis and its detoxification. International Journal of Science 3, 18-23.

Bennett A, Bogorad L. 1973. Complementary chromatic adaption in a filamentous blue-green alga. Journal of Cell Biology 58, 419-435.

Bilal M, Shah JA, Ashfaq T, Gardazi SMH, Tahir AA, Pervez A, Haroon H, Mahmood Q. 2013. Waste biomass adsorbents for copper removal from industrial wastewater. Journal of Hazardous Materials 263, 322-333.

Bradford MM. 1979. A rapid and sensitive method for the quantisation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.

Bryant AD. 1994. The Molecular Biology of Cyanobacteria. Kluwer Academic Publishers, Norwell, MA, USA, 559-575.

Bussche JV, Soares EV. 2011. Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae. Applied Microbiology & Biotechnology 90, 679-687.

Carpentier S, Moilleron R, Thevenot D, Beltran C, Herve D. 2001. Environmental impact of laying in water degraded sediment. Houille Blanche 8, 82-86.

Chaloub RM, de Magalhaes CCP, dos Santos CP. 2005. Early toxic effects of zinc on PSII of Synechocystis aquatilis f. aquatilis (Cyanophyceae). Jourrnal of Phycology 41, 1162-1168.

Chen C, Wang JL. 2007. Response of Saccharomyces cerevisiae to lead ion stress. Applied Microbiology & Biotechnology 74, 683-687.

Cui QH, Tang CC. 2000. Effects of lead and selenium on yeast (Saccharomyces cerevisiae) telomere. Journal of Environment Science & Health A 35, 1663-1671.

Danilov RA, Ekelund NGA. 2001. Effects of Cu2+, Ni2+, Pb2+, Zn2+ and penta-chlorophenol on photosynthesis and motility in Chlamydomonas reinhardtii in short-term exposure experiments. BMC Ecology 1, 1. DOI: 10.1186/1472-6785-1-1.

Diengdoh OL, Syiem MB, Pakshirajan K, Rai AN. 2017. Zn2+ sequestration by Nostoc muscorum: study of thermodynamics, equilibrium isotherms and biosorption parameters for the metal. Environment Monitoring & Assessment 189, 314. DOI: 10.1007/s10661-017-6013-4.

Dikshith TSS. 2009. Safe use of chemicals: a practical guide, United States of America (USA): CRC Press: From Cu (II)-Co (II) single and binary mixtures on brown algae C. indica. Journal of Environment Chemistry & Engineering 3, 140-149. http://dx.doi.org/10.1016/j.jece. 2014.11.004.

Elbert W, Weber B, Brrows S, Steinkamp J, Budel B, Andrea MO, Poschl U. 2012. Contribution of cryptogamic covers to the global cycles of carbon and nitrogen. Nature Geoscience 5, 459-462.

Goswami S, Syiem MB, Pakshirajan K. 2015. Cadmium removal by Anabaena doliolum Ind1 isolated from a coal mining area in Meghalaya, India: associated structural and physiological alterations. Environment Engineering & Research 20, 41-50.

Gupta VK, Rastogi A. 2008. Biosorption of lead from aqueous solutions by green algae Spirogyra species: Kinetics and equilibrium studies. Journal of Hazardous Materials 152, 407-414.

Hader DP, Kumar HD, Smith RC, Worrest RC. 2007. Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochemistry and Photobiological Science 6, 267-285.

Heng L, Jusoh YK, Ling CHM, Idris M. 2004. Toxicity of single and combinations of lead and cadmium to the cyanobacteria Anabaena flos-aquae. Bulletin of Environment Contamination & Toxicology 72, 373-379.

Hesler A. 1974. Effect of lead on algae. Water Air Soil Pollution 3, 371-385.

James D and Cook MD. 1983. Determinants of non-heme iron adsorption in man. Food Technology p 124-126.

Jin YN, Clark AB, Slebos RJC, Al-Refai H, Taylor JA, Kunkel A, Resnick MA, Gordenin DA. 2003. Cadmium is a mutagen that acts by inhibiting mismatch repair. Nature Genetics 34, 326-329. DOI: 10.1038/ng1172.

Kelly M. 1988. Uptake and Accumulation of Heavy Metals. In: Kelly, M., Allison, WJ, Garman AR, Symon CJ, Ed. Mining and the freshwater environment. Springer, Netherlands., p. 43-63. DOI. 10.1007/978-94-009-1359-2.

Kojima Y, Hiyama T, Sakurai H. 1987. Effects of mercurial on iron-sulfur centers of Phtosystem I of Anacystis nidulans. In: Biggins J, Ed. Progress in Photosysnthesis Research. Nijhoff/Junk Publishers, The Hague, The Netherlands. Vol. 2, p. 57-60.

Krupa Z, Baszyriski T. 1995. Some aspects of heavy metal toxicity towards photosynthetic apparatus-direct and indirect effects on light and dark reactions. Acta Physiology Plantae 17, 177-190.

Mackinney G. 1941. Absorption of light by chlorophyll solutions. Journal of Biological Chemistry 140, 315-322.

Mesquita VA, Machado MD, Silva CF, Soares EV. 2016. Influence of the metabolic state on the tolerance of Pichia kudriavzevii to heavy metals. Journal of Basic Microbiology 56, 1244-1251. DOI: 10.1002/jobm.201600232.

Morgan RC. 1967. The carotenoids of Queensland fruits. Carotenes of the watermelon (Citrullus vulgaris). Journal of Food Science 32, 275-278.

Nagarajan A, Burnap RL. 2014. Parallel expression of- alternate forms of PsbA2 gene provide evidence for the existence of a targeted D1 repair mechanism in Synechocystis sp. PCC 6803. Biochimie and Biophysical Acta 1837, 1417-1426.

Naja GM, Volesky B. 2010. Treatment of metal bearing effluents: removal and recovery. In: Wang LK, Chen JP, Hung YT, Shammas NK. Ed. Handbook on heavy metals in the environment. Taylor and Francis, Boca Raton., p. 247-291.

Osmond CB. 1981. Photorespiration and Photoinhibition. Biochimie Biophysical Acta 639, 77-98.

Perez RR, Sousa CA, Vankeersbilck T, Machado MD, Soares EV. 2013. Evaluation of the role of glutathione in the lead-induced toxicity in Saccharomyces cerevisiae. Current Microbiology 67, 300-305.

Pinchasov Y, Berner T, Dubinsky Z. 2006. The effect of lead on determined by photo acoustics in Synechococcus leopoliensis. Air Soil Pollution 175, 117-125.

Poskuta JW, Parys E, Romanowska E. 1996. Toxicity of lead to photosynthesis, accumulation of chlorophyll, respiration and growth of Chlorella pyrenoidosa protective role of dark respiration. Acta Physiology Plantae 18, 165-171.

Prasanna R, Sood A, Rath SK, Singh PK. 2014. Cyanobacteria as a green option for sustainable agriculture. In: Sharma NK, Rai AK, Stal LJ, Ed. Cyanobacteria: an economic perspective. Wiley, London., p 145-166.

Qaiser S, Saleemi, Ahmad AR. 2007. Heavy metal uptake by agro based waste materials. Journal of Biotechnology 10, 409-416.

Reddy DHK, Lee SM, Seshaiah K. 2012. Removal of Cd(II) and Cu(II) from aqueous solution by agro biomass equilibrium, kinetic and thermodynamic studies. Environment Engineering & Research 17, 125-132.

Rippka R, Dereulles J, Waterbury JB. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111, 1-61.

Robinson SJ, Deroo CS, Yocum CF. 1982. Photosynthetic electron transfer in preparations of cyanobacterium Spirulina platensis. Plant Physiology 70, 154-161.

Roe JH. 1955. The determination of sugar in blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry 212, 335-343.

Rossi E. 2008. Low level environmental lead exposure – a continuing challenge. Clinical Biochemical Review 29, 63-70.

Santos RW, Schmidt EC, Martins RDP, Latini A, Maraschin M, Horta PA, Bouzon ZL. 2012. Effects of cadmium on growth, photosynthetic pigments, photosynthetic performance, biochemical parameters and structure of chloroplasts in the Agarophyte Gracilaria domingensis (Rhodophyta gracilariales). American Journal of Plant Sciences 3, 1077-1084. Doi: 10.4236/ajps.2012.38129.

Silbergeld EK, Waalkes M, Rice JM. 2000. Lead as a carcinogen: experimental evidence and mechanisms of action. American Journal of Industrial Medicine 38, 316-323.

Singh RP, Tripathi RD, Sinha SK,Maheshwari R, Srivastava HS. 1997. Response of higher plants to lead contaminated environment. Chemosphere  34, 2467-2493.

Singh S, Parihar P, Singh R, Singh VP, Prasad SM. 2016. Heavy metal tolerance in plants: role of transcriptomics, proteomics, metabolomics, and ionomics. Frontier of Plant Science 6, 1143.

Smedley PL, Nicolli HB, Macdonald DMJ, Barros AJ, Tullio JO. 2002. Hydrogeochemistry of arsenic and other inorganic constituents in ground waters from La Pampa, Argentina. Applied Geochemistry 17, 259-284.

Soares EV, Hebbelinck  K, Soares HMVM. 2003. Toxic effects caused by heavy metals in the yeast Saccharomyces cerevisiae: a comparative study. Canadian Journal of Microbiology 49, 336-343.

Sousa CA, Soares EV. 2014. Mitochondria are the main source and one of the targets of Pb (lead)-induced oxidative stress in the yeast Saccharomyces cerevisiae. Applied Microbiology & Biotechnology 98, 5153-5160.

Stratton GW, Huber AL,Corke CT. 1979. Effect of mercuric ions on growth, photosynthesis and nitrogenase activity of Anabaena equalis. Applied Environmental Microbiology 38, 537-543.

Surosz W, Palinska KA. 2004. Effect of heavy metal stress on cyanobacterium Anabaena flos-aquae. Archieve of Environment Contamination & Toxicology 48, 40-48.

Umena Y, Kamakami K, Shen JR, Kamiya N. 2011. Crystal structures of oxygen evolving photosystem II at a resolution of 1.9 A°. Nature 473, 55-60. doi: 10.1038/nature09913.

USEPA. 2006. Air Quality criteria for lead (Final Report, 2006). U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-05/144aF-bF.

Van der Heggen M, Martins S, Flores G, Soares EV. 2010. Lead toxicity in Saccharomyces cerevisiae. Applied Microbiology & Biotechnology 88, 1355-1361.

Veglio F, Beolchini F. 1997. Removal of metals by biosorption: a review. Hydrometal 44, 301-316.

Vymazal J. 1990. Toxicity and accumulation of lead with respect to algae and cyanobacteria: a review. Acta Hydrochimie Hydrobiology 18, 513-535.

Vymazal J. 1995. Algae and element cycle in Wetlands. Chelsea, Lewis, USA.

Wen-Xiong W. 2002. Interactions of trace metals and different marine food chains. Marine Ecology 243, 295-309.

Whitton BA. 2012. Introduction to the cyanobacteria. In: Whitton BA., Ed. Ecology of Cyanobacteria II: Their diversity in space and time. Springer Netherlands., p. 1-13.

Wong D, Govindjee G. 1976. Effects of lead ions on photosystem I in isolated chloroplast: Studies on the reaction centre P700. Photosynthetica 10, 241-254.

Wuana RA, Okiaimen FE, Imborvunga JA. 2010. Removal of heavy metals from a contaminated soil using organic chelating acids. International Journal of Environment Science & Technology 7, 485-496.

Yu Y, Shapter JG, Popelka-Filcoff R, Bennett JW, Ellis AV. 2014. Copper removal using bio-inspired polydopamine coated natural zeolites. Journal of Hazardous Materials 273, 174-182. http://dx.doi.org/10.1016/j.jhazmat.2014.03.048.