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A potential antimicrobial, extracellular enzymes, and antioxidants resource: Endophytic fungi associated with medicinal plants

Eman A. Attia, Bhim P.Singh, Kavya Dashora, Ahmed M. Abdel-Azeem

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Int. J. Micro. Myco.13(1), 26-38, July 2021

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Abstract

This study aimed for the production of antimicrobial, extracellular enzymes and antioxidants by endophytic teleomorphic Ascomycota associated with medicinal plants. A total of eleven teleomorphic species were isolated from four medicinal plant species. Chaetomium grande and Sordaria fimicola were the most frequently isolated species and represented by 12 (Chg1-Chg12) and 7 (Sf1-Sf7) isolates respectively. The minimum inhibitory concentration (MIC) of all the isolates was determined against nine reference strains of bacteria and fungi. Effectiveness of 100-300µg/ ml DEMSO then in H2O of the ethyl acetate fractions of the most effective two isolates Chg 5 and Sf 3 on the tested reference strains revealed a different inhibitory effecst. Saturated disc of Streptomycin and Rifampin (0.165mg/ml) were used for bacteria and amphotericin B and fluconazole were used for yeasts and fungi as a positive control. Enzymatically, Chg 5 isolate considered as a resource of amylase, cellulase, protease, lipase, and chitinase. however, Sf3 isolates considered as a resource of amylase, laccase, and chitinase out of six screened enzymes. Total phenolics (TP), total flavonoids (TF) and antioxidant activity of the Sf3 and Chg 5 extracs were measured. The TP values were expressed as milligram gallic acid equivalents per gram of dry extract of Sf3 and Chg 5, which equal to 53.9±0.35 and 97.9±0.48 respectively. TF present in both Sf3 and Chg5 isolates extracts with values equal to 2.44±0.01 and 7±0.05 respectively expressed as routine equivalents. in vitro, the antioxidant activity of the extracts was investigated using DPPH radical-scavenging assay, and equal to 0.06% and 0.39% respectively in the extract of both taxa.

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A potential antimicrobial, extracellular enzymes, and antioxidants resource: Endophytic fungi associated with medicinal plants

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