A potential Antimicrobial, Extracellular Enzymes, and Antioxidants resource: Endophytic fungi associated with medicinal plants

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A potential Antimicrobial, Extracellular Enzymes, and Antioxidants resource: Endophytic fungi associated with medicinal plants

Eman A. Attia, Bhim P. Singh, Kavya Dashora, Ahmed M. Abdel-Azeem
Int. J. Biosci.17( 1), 119-132, July 2020.
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This study aimed for the production of antimicrobial, extracellular enzymes and antioxidants by endophytic teleomorphic Ascomycota associated with medicinal plants. A total of eleven teleomorphic species were isolated from four medicinal plant species. Chaetomium grande and Sordaria fimicola were the most frequently isolated species and represented by 12 (Chg1-Chg12) and 7 (Sf1-Sf7) isolates respectively. The minimum inhibitory concentration (MIC) of all the isolates was determined against nine reference strains of bacteria and fungi. Effectiveness of 100-300 µg/ ml DEMSO then in H2O of the ethyl acetate fractions of the most effective two isolates Chg5 and Sf3 on the tested reference strains revealed a different inhibitory effecst. Saturated disc of Streptomycin and Rifampin (0.165 mg/ml) were used for bacteria and amphotericin B and fluconazole were used for yeasts and fungi as a positive control. Enzymatically, Chg5 isolate considered as a resource of amylase, cellulase, protease, lipase, and chitinase. However, Sf3 isolates considered as a resource of amylase, laccase, and chitinase out of six screened enzymes. Total phenolics (TP), total flavonoids (TF) and antioxidant activity of the Sf3 and Chg5 extracs were measured. The TP values were expressed as milligram gallic acid equivalents per gram of dry extract of Sf3 and Chg5, which equal to 53.9±0.35 and 97.9±0.48 respectively. TF present in both Sf3 and Chg5 isolates extracts with values equal to 2.44±0.01 and 7±0.05 respectively expressed as routine equivalents. In vitro, the antioxidant activity of the extracts was investigated using DPPH radical-scavenging assay, and equal to 0.06% and 0.39% respectively in the extract of both taxa.


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