Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

Paper Details

Research Paper 01/06/2019
Views (651)
current_issue_feature_image
publication_file

Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

Marie Mae Angelica C. Tolentino, Jayson T. Francisco
Int. J. Biosci. 14(6), 250-256, June 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Cellulose from Gigantochloa levis leaf and branch were extracted and hydrolyzed in acidic medium to produce reducing sugars at different reaction times (30, 60, 90, 120 minutes). The extraction of cellulose from G. levis leaf and branch were carried out by a three-step delignification process. The extracted cellulose from both samples were characterized via FTIR, morphological analysis and test for lignin using photomicrograph. Furthermore, crude cellulose from both samples were hydrolyzed to produce reducing sugars. The produced reducing sugars were qualitatively confirmed using Benedict’s test, then quantitatively determined through Lane- Eynon titration. Significant difference on the percentage yield of the produced cellulose and on the amount of reducing sugars produced at various reaction times were also determined. The extracted crude cellulose from G. levis leaf and branch were white in appearance, having yields of 19.1897 ± 0.2907% and 19.0183 ± 0.7095%, respectively. FTIR spectra of both cellulose samples do not show a C=C stretching and C=O stretching vibrations while the chemical test for lignin of both samples gave a negative result. Both results imply that lignin and hemicellulose were successfully removed. Meanwhile, the amount of reducing sugars for both samples increased from 30 to 90 minutes but decreases beyond the 90- minute reaction time. There is no significant difference in the percentage yield of both cellulose while there exists a significant difference in the amount of reducing sugars produced at various reaction time. Overall, the results suggested that the G. levis is a possible source of cellulose and its corresponding hydrolysates.

Aboody MH. 2013. Extraction of cellulose from some industrial and plant’s waste and its hydrolysis using new heterogeneous catalyst. MS Thesis. University of Baghdad.

Ayeni AO, Adeeyo OA, Oresegun OM, Oladimeji E. 2015. Compositional Analysis of Lignocellulosic Materials: Evaluation of an Economically Viable Method Suitable for Woody and Non-Woody Biomass. American Journal of Engineering Research 4, 14-19.

Dai L, Long Z, Lv Y, Feng Q. 2014. The Role of Formic Acid Pretreatment in Improving the Carboxyl Content of Tempo- Oxidized Cellulose. Cellulose Chemistry and Technology 48, 5- 6.

dela Rosa S, Martin J, Fierro J. 2014. Optimization of the process of chemical hydrolysis of cellulose to glucose. Cellulose 21(4), 2397- 2407. https://doi.org/10.1007/s10570-014-0280-9

Ma X, Huang L, Cao S, Chen Y, Luo X, Chen L. 2012. Preparation of Dissolving Pulp from Bamboo for Textile Applications. Part 2. Optimization of Pulping Conditions of Hydrolyzed Bamboo and its Kinetics. Bioresources. 7(2), 1866-1875. http://doi.org/10.15376/biores.7.2.1866-1875

Messiry ME. 2014. Morphological Analysis of Micro-Fibrillated Cellulose from Different Raw Materials for Fiber Plastic Composites. Journal of Textile Science and Engineering 4(5), 2-7. http://doi.org/10.4172/2165-8064.1000166

Moran J, Alvarez V, Cyras V, Vazquez A. 2008. Extraction of cellulose and preparation of nanocellulose from sisal fibers. Cellulose 15(1), 149-159. https://doi.org/10.1007/s10570-007-9145-9

Pandiar D, Baranwal HC, Kumar S, Ganesan V, Sonkar PK, Chattopadhyay K. 2017. Use of Jaggery and Honey as Adjunctive Cytological Fixatives to Ethanol for Oral Smears. Journal of Oral and Maxillofacial Pathology 21(2), 317. https://doi.org/10.4103/jomfp.JOMFP_224_15

Qian X. 2012. Mechanisms and Energetics for Brønsted Acid-Catalyzed Glucose Condensation, Dehydration and Isomerization Reactions. Topics in Catalysis 55(3-4), 218-226. https://doi.org/10.1007

Saha B, Iten L, Cotta M, Wu V. 2005. Dilute Acid Pretreatment, Enzymatic Saccharification and Fermentation of Wheat Straw to Ethanol. Process Biochemistry 40(12), 3693- 3700. https://doi.org /10.1016/j.procbio.2005.04.006

Stange Jr R, Alessandro R, Mc Collum G, Mayer R. 2002. Studies on the phloroglucinol- HCl reactive material produced by squash fruit elicited with pectinase: isolation using hydrolytic enzymes and release of p– coumaryl aldehyde by water reflux. Physiological and Molecular Plant Pathology 60(6), 283-29. https://doi.org/10.1006/pmpp.2002.0406

Truong A, Anh Le T. 2014. Overview of Bamboo Biomass for Energy Production. University of Sciences and Technologies of Hanoi, Vietnam, 5.

Woldu AR, Tsigie YA. 2015. Optimization of Hydrolysis for Reduced Sugar Determination from Avocado Seed Wastes. American Journal of Environment, Energy and Power Research 3(1), 1-10.

Related Articles

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.

Agronomic performance and profitability of coffee wildlings using different soil media mixtures

Maribel L. Fernandez, Ricardo B. Casauay, Ronel A. Collado, Int. J. Biosci. 27(2), 189-199, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 176-188, August 2025.

Design and development of solar powered water sprayer: A green technology innovation

Lorenzo V. Sugod, Int. J. Biosci. 27(2), 159-175, August 2025.

Knowledge, attitudes, practices, and social awareness regarding SARS-CoV-2 infection in the kyrgyz population in the post-pandemic period

Mirza Masroor Ali Beg, Haider Ali, Yahya Nur Ahmed, Yavuz Gunduz, Hafsa Develi, Tilekeeva UM, Int. J. Biosci. 27(2), 151-158, August 2025.

Tumor suppressing ability of myrtenal in DMBA-induced rat mammary cancer: A biochemical and histopathological evaluation

Manoharan Pethanasamy, Shanmugam M. Sivasankaran, Saravanan Surya, Raju Kowsalya, Int. J. Biosci. 27(2), 141-150, August 2025.

Assessing tree diversity in cashew plantations: Environmental and agronomic determinants in buffer zones of Mont Sangbé National Park, western Côte d’Ivoire

Kouamé Christophe Koffi, Kouakou Hilaire Bohoussou, Serge Cherry Piba, Naomie Ouffoue, Sylvestre Gagbe, Alex Beda, Adama Tondossama, Int. J. Biosci. 27(2), 122-133, August 2025.