Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | June 1, 2019

| Download

Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

Marie Mae Angelica C. Tolentino, Jayson T. Francisco

Key Words:

Int. J. Biosci.14(6), 250-256, June 2019

DOI: http://dx.doi.org/10.12692/ijb/14.6.250-256


IJB 2019 [Generate Certificate]


Cellulose from Gigantochloa levis leaf and branch were extracted and hydrolyzed in acidic medium to produce reducing sugars at different reaction times (30, 60, 90, 120 minutes). The extraction of cellulose from G. levis leaf and branch were carried out by a three-step delignification process. The extracted cellulose from both samples were characterized via FTIR, morphological analysis and test for lignin using photomicrograph. Furthermore, crude cellulose from both samples were hydrolyzed to produce reducing sugars. The produced reducing sugars were qualitatively confirmed using Benedict’s test, then quantitatively determined through Lane- Eynon titration. Significant difference on the percentage yield of the produced cellulose and on the amount of reducing sugars produced at various reaction times were also determined. The extracted crude cellulose from G. levis leaf and branch were white in appearance, having yields of 19.1897 ± 0.2907% and 19.0183 ± 0.7095%, respectively. FTIR spectra of both cellulose samples do not show a C=C stretching and C=O stretching vibrations while the chemical test for lignin of both samples gave a negative result. Both results imply that lignin and hemicellulose were successfully removed. Meanwhile, the amount of reducing sugars for both samples increased from 30 to 90 minutes but decreases beyond the 90- minute reaction time. There is no significant difference in the percentage yield of both cellulose while there exists a significant difference in the amount of reducing sugars produced at various reaction time. Overall, the results suggested that the G. levis is a possible source of cellulose and its corresponding hydrolysates.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

Aboody MH. 2013. Extraction of cellulose from some industrial and plant’s waste and its hydrolysis using new heterogeneous catalyst. MS Thesis. University of Baghdad.

Ayeni AO, Adeeyo OA, Oresegun OM, Oladimeji E. 2015. Compositional Analysis of Lignocellulosic Materials: Evaluation of an Economically Viable Method Suitable for Woody and Non-Woody Biomass. American Journal of Engineering Research 4, 14-19.

Dai L, Long Z, Lv Y, Feng Q. 2014. The Role of Formic Acid Pretreatment in Improving the Carboxyl Content of Tempo- Oxidized Cellulose. Cellulose Chemistry and Technology 48, 5- 6.

dela Rosa S, Martin J, Fierro J. 2014. Optimization of the process of chemical hydrolysis of cellulose to glucose. Cellulose 21(4), 2397- 2407. https://doi.org/10.1007/s10570-014-0280-9

Ma X, Huang L, Cao S, Chen Y, Luo X, Chen L. 2012. Preparation of Dissolving Pulp from Bamboo for Textile Applications. Part 2. Optimization of Pulping Conditions of Hydrolyzed Bamboo and its Kinetics. Bioresources. 7(2), 1866-1875. http://doi.org/10.15376/biores.7.2.1866-1875

Messiry ME. 2014. Morphological Analysis of Micro-Fibrillated Cellulose from Different Raw Materials for Fiber Plastic Composites. Journal of Textile Science and Engineering 4(5), 2-7. http://doi.org/10.4172/2165-8064.1000166

Moran J, Alvarez V, Cyras V, Vazquez A. 2008. Extraction of cellulose and preparation of nanocellulose from sisal fibers. Cellulose 15(1), 149-159. https://doi.org/10.1007/s10570-007-9145-9

Pandiar D, Baranwal HC, Kumar S, Ganesan V, Sonkar PK, Chattopadhyay K. 2017. Use of Jaggery and Honey as Adjunctive Cytological Fixatives to Ethanol for Oral Smears. Journal of Oral and Maxillofacial Pathology 21(2), 317. https://doi.org/10.4103/jomfp.JOMFP_224_15

Qian X. 2012. Mechanisms and Energetics for Brønsted Acid-Catalyzed Glucose Condensation, Dehydration and Isomerization Reactions. Topics in Catalysis 55(3-4), 218-226. https://doi.org/10.1007

Saha B, Iten L, Cotta M, Wu V. 2005. Dilute Acid Pretreatment, Enzymatic Saccharification and Fermentation of Wheat Straw to Ethanol. Process Biochemistry 40(12), 3693- 3700. https://doi.org /10.1016/j.procbio.2005.04.006

Stange Jr R, Alessandro R, Mc Collum G, Mayer R. 2002. Studies on the phloroglucinol- HCl reactive material produced by squash fruit elicited with pectinase: isolation using hydrolytic enzymes and release of p– coumaryl aldehyde by water reflux. Physiological and Molecular Plant Pathology 60(6), 283-29. https://doi.org/10.1006/pmpp.2002.0406

Truong A, Anh Le T. 2014. Overview of Bamboo Biomass for Energy Production. University of Sciences and Technologies of Hanoi, Vietnam, 5.

Woldu AR, Tsigie YA. 2015. Optimization of Hydrolysis for Reduced Sugar Determination from Avocado Seed Wastes. American Journal of Environment, Energy and Power Research 3(1), 1-10.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background