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Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

Research Paper | June 1, 2019

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Marie Mae Angelica C. Tolentino, Jayson T. Francisco

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Int. J. Biosci.14( 6), 250-256, June 2019

DOI: http://dx.doi.org/10.12692/ijb/14.6.250-256


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Cellulose from Gigantochloa levis leaf and branch were extracted and hydrolyzed in acidic medium to produce reducing sugars at different reaction times (30, 60, 90, 120 minutes). The extraction of cellulose from G. levis leaf and branch were carried out by a three-step delignification process. The extracted cellulose from both samples were characterized via FTIR, morphological analysis and test for lignin using photomicrograph. Furthermore, crude cellulose from both samples were hydrolyzed to produce reducing sugars. The produced reducing sugars were qualitatively confirmed using Benedict’s test, then quantitatively determined through Lane- Eynon titration. Significant difference on the percentage yield of the produced cellulose and on the amount of reducing sugars produced at various reaction times were also determined. The extracted crude cellulose from G. levis leaf and branch were white in appearance, having yields of 19.1897 ± 0.2907% and 19.0183 ± 0.7095%, respectively. FTIR spectra of both cellulose samples do not show a C=C stretching and C=O stretching vibrations while the chemical test for lignin of both samples gave a negative result. Both results imply that lignin and hemicellulose were successfully removed. Meanwhile, the amount of reducing sugars for both samples increased from 30 to 90 minutes but decreases beyond the 90- minute reaction time. There is no significant difference in the percentage yield of both cellulose while there exists a significant difference in the amount of reducing sugars produced at various reaction time. Overall, the results suggested that the G. levis is a possible source of cellulose and its corresponding hydrolysates.


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Acid-mediated hydrolysis of cellulose from Gigantochloa levis (Bolo) leaf and branch

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