Agrobacterium tumefaciens pathogenicity and characterization in rose crown gall disease

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Research Paper 08/04/2024
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Agrobacterium tumefaciens pathogenicity and characterization in rose crown gall disease

Zohaib Ahmad, Mohammed Abdulaziz Alfehaid, Sidra Gill, Muhammad Atif, Abdul Manan, Zain-Ul-Abadin
Int. J. Biosci.24( 4), 129-135, April 2024.
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Abstract

Agrobacterium is a bacterium that has a gigantic role in modern plant genetic engineering. As its beneficial role of the bacterium, it also causes crown gall diseases in many horticultural crops and causes losses all over the world. A crown gall-infected rose sample was collected and morphological, biochemical, and cultural characteristics using NA, YDC, YPDA and King’s B mediums. Different tests were conducted to study the biochemical properties of the bacterium such as gram staining, KOH solubility test, Starch hydrolysis, Kovac’s oxidase, Litmus milk, Hydrolysis of gelatin, Citrate utilization, Methyl red and Voges Proskauer test, fluorescent pigment, Urease and Catalase tests. A pathogenicity test was also performed on broad bean plants in the greenhouse to confirm the pathogen. All these test results exhibited that the crown gall disease in rose due to the attack of the Agrobacterium tumefaciens. These tests are essential to identify the pathogenic bacterium to develop the management strategies for the control of the pathogen. Several reports indicate that this soil-born pathogen is an emerging threat to several crops of horticulture as well as to ornamental plants around the globe.

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