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Research Paper | July 1, 2013

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An efficient DNA extraction protocol for medicinal plants

Ajmal Iqbal, Israr Ahmad, Habib Ahmad, M. S. Nadeem, Mohammad Nisar, Huma Riaz

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Int. J. Biosci.3(7), 30-35, July 2013

DOI: http://dx.doi.org/10.12692/ijb/3.7.30-35

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Abstract

This paper communicates a simple and cost effective protocol for isolation of genomic DNA from dry parts of Berberis and Mentha. This protocol was applied to 5 species of Berberis and 4 species of Mentha collected from different locality of Kunhar Valley. In this protocol 5 M NaCl, 2% CTAB, 1% PVP and 0.1 % ß-mercaptoethanol were used and incubated at 600C for 25 minutes. Pure DNA extracted by this method was found sufficient and suitable for PCR amplification and Southern blot hybridization analyses, which are the important steps in crop improvement programme through marker development and genetic engineering techniques.

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An efficient DNA extraction protocol for medicinal plants

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