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Analysis of the differential effects of methyl jasmonate on induction of adventitious roots and antioxidant potential in Artimisia scoparia

Research Paper | August 1, 2019

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Asghar Khan, Mubarak Ali Khan, Maqsood Alam, Raj Akbar, Amir Ali, Sher Mohammad, Ijaz Naeem, Mamoona Rauf

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Int. J. Biosci.15( 2), 547-558, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.547-558


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Artemisia scoparia is an important medicinal plant, having plenty of pharmacological applications. In this study the comparative effects of auxins and elicitors were investigated on the induction, biomass formation and antioxidant potential of adventitious roots (AR) in leaf explants on solid MS media, followed by studying the growth kinetics in suspension cultures. It was observed that among the different auxins employed in vitro, NAA showed highest induction frequency (57%) when employed at 1.0mg/L on solid MS media. Within the elicitors, 0.5mg/L Me-J inducted maximum roots induction frequency (58%), while 1.0mg/LPAA resulted in 43% induction frequency. During AR suspension cultures, in response to 1.0mg/L NAA, shorter lag and stationary phases of 8 and 4 days respectively were observed in the growth curve. The log phase was quite longer consisting of 24 days, wherein highest biomass (7.36g/L; DW) was accumulated on 28 day of the growth curve. Within the different growth phases, day 20 (log phase) was observed to accumulate higher antioxidant activity in the AR suspension cultures than other growth stages. Lesser antioxidant activity was determined in the stationary phase of cell cycle. Highest antioxidant potential (87%) was recorded in the AR suspension cultures raised in presence of 0.5mg/L Me-J. This protocol has the potential for commercial production of antioxidants enriched biomass of A. scoparia.


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Analysis of the differential effects of methyl jasmonate on induction of adventitious roots and antioxidant potential in Artimisia scoparia

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