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Antagonist effect of volatile organic compounds produced by Debaryomyces hansenii on Colletotrichum gloeosporoides as anthracnose reason of tropical apples

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Research Paper 01/07/2017
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Antagonist effect of volatile organic compounds produced by Debaryomyces hansenii on Colletotrichum gloeosporoides as anthracnose reason of tropical apples

D. Indratmi, I. R. Sastrahidayat, A. L. Abadi, S. Djauhari
J. Bio. Env. Sci.11( 1), 114-122, July 2017.
Certificate: JBES 2017 [Generate Certificate]
Key: AntrachnoseAppleColletotrichum gloeosporoidesDebaryomyces hanseniiVolatile organic compounds

Abstract

The usage antagonist yeast for biological control had emerged as one of the most promising alternatives in pre and postharvest protection of apples in Indonesia. The aim of the research was to identify volatile organic compounds (VOCs) produced by Debaryomyces hansenii that inhibited the growth of Colletotrichum gloeosporioides, pathogenic fungi of tropical apples. D. hansenii was isolated from apples in Indonesian.  Bioassay using potato-dextrose agar medium in sealed petri dishes showed that the fungi growth inhibition was ultimately due to volatile organic compounds produced by D. hansenii. The VOCs were trapped on activated charcoal prior to analyzing by integrated thermal desorption using GC-MS. The VOCs significantly inhibited the mycelia growth of C. gloeosporioides by 85.07 %, and induced morphological abnormalities such as mycelia deviations. The identified VOCs include acids, esters, ketones, oximes, heterocyclic, and phthalate compounds. The result indicated that tropical apple yeast is abundant resources of bioactive VOCs and played an important role in reducing apple anthracnose disease of tropical apples in Indonesia.

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Arfi K, Spinnle HE, Tache R, Bonnarme P. 2002. Production of volatile compounds by cheese-ripening yeasts: requirement for a methanethiol donor for S-methyl thioacetate synthesis by Kluyveromyces lactis. Appl Microbiol Biotechnol 58, 503–510.

Blevea G, Griecoa F, Cozzib G, Logrieco A, Viscontib A.  2006.   Isolation of epiphytic yeasts with potential for biocontrol of Aspergillus carbonarius and A. niger on grape. International Journal of Food Microbiology 108(2), 204–209.

Breuer U, Harms H.  2006.  Yeast.   Yeast 23, 415–437.

Bruce A, Verrall S, Hackett CA,  Wheatley RE. 2005. Identification of volatile organic compounds (VOCs) from bacteria and yeast causing growth inhibition of sapstain fungi. Holzforschung 58, 193-198.

Chaurasia B, Pandey A, Palni LMS, Trivedi P, Kumar B, Colvin N.  2005. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological Research 160(1), 75–81.

Dura MA, Flores M, Toldra F. 2004. Effect of growth phase and dry-cured sausage processing conditions on Debaryomyces spp. generation of volatile compounds from branched-chain amino acids. Food Chem. 86, 391–399.

Francesco AD, Ugolini L, Lazzeri L, Mari M. 2014. Production of volatile organic compounds by Aureobasidium pullulans as a potential mechanism of action against postharvest fruit pathogens. Biological Control 81, 8-14.

Gori K, Mortensen HD, Arneborg N, Jespersen L. 2007. Ammonia production and its possible role as a mediator of communication for Debaryomyces hansenii and other cheese-relevant yeast species. Journal of Dairy Science 90(11), 5032-5041.

Hartati S, Wiyono S, Hidayat SH,  Sinaga MS. 2015. Mode of Action of Yeast-Like Fungus Aureobasidium pullulans in Controlling Anthracnose of Postharvest Chili. IJSBAR 20( 2), 253-263

Huang R, Li GQ, Zhang J, Yang L, Che HJ, Jiang DH, Huang HC. 2011. Disease Control and Pest Management Control of Postharvest Botrytis Fruit Rot of Strawberry by Volatile Organic Compounds of Candida intermedia. Phytopathology 101, 859-869.

Janisiewicz WJ, Tworkoski TJ, Kurtzman CP.  2001. Biocontrol potential of Metchnikowia pulcherrima strains against blue mold of apple.  Phytopathology 91(11), 1098–1108.

Jiménez-Moreno N, Ancín-Azpilicueta C. 2009. Sorption of volatile phenols by yeast cell walls. International Journal of Wine Research 1, 11-18.

Korpi A, Jarnberg J, Pasanen AL. 2009. Microbial volatile organic compounds. Critical Reviews in Toxicology 39, 139-193.

Medina-Córdova N, López-Aguilar R, Ascencio F, Castellanos T, Campa-Córdova AI, Angulo C. 2016.  Biocontrol activity of the marine yeast Debaryomyces hansenii against phytopathogenic fungi and its ability to inhibit mycotoxins production in maize grain (Zea mays L.).    Biological Control 97, 70–79.

Montealegre JR, Reyes R, Perez LM, Herrera R, Silva P, Besoain X. 2003.  Selection of Bioantagonistic Bacteria to be Used in Biological Control of Rhizoctonia solani in Tomato.  J. Biotechnol.  6, 116-127.

Pub Chem, 2015. National Center for Biotechnology Information. PubChem Compound Database; CID=5489487, (accessed Nov. 26, 2015). https://pubchem.ncbi.nlm.nih.gov/compound/5489487

Rahman MA, Kadir J, Mahmud TMM, Rahman RA, Begum MM. 2007. Screening of Antagonistic Bacteria for Biocontrol Activities on Colletotrichum gloeosporioides in Papaya. Asian Journal of Plant Sciences 6, 12-20.

Salgado ST, Tinoco R, Vazquez-Duhalt R, Caballero-Mellado J, Perez-Rueda E. 2013. Identification of volatile compounds produced by the bacterium Burkholderia tropica that inhibit the growth of fungal pathogens. Bioengineered 4(4), 236–243.

Starmer WT, Lachance M. 2011. Yeast ecology. In Kurtzman C.P., Fell J.W., Boekhout, T. (Eds.). The yeasts: A taxonomic study. 5th ed. Amsterdam, Elsevier, 65-83.

Sultan MZ, Moon SS, Park K.  2010. Natural Phthalate Derivatives from the Bacterium Burkholderia cepacia K87. J. Sci. Res. 2(1), 191-195.

Sultan Z, Park K, Lee SY, Park JK, Varughese Moon SS. 2008. Novel oxidized derivatives of antifungal pyrrolnitrin from the bacterium Burkholderia cepacia K87. J Antibiot (Tokyo) 61, 420–425.

Tawfik KA, Jeffs P, Bray B, Dubay G, Falkinham JO, Mesbah M. 2010. Burkholdines 1097 and 1229, potent antifungal peptides from Burkholderia ambifaria 2.2N. Org Lett. 12, 664–666.

Urquhart EJ, Punja ZK. 2002. Hydrolytic enzymes and antifungal compounds produced by Tilletiopsis species, phyllosphere yeasts that are antagonists of powdery mildew fungi. Canadian Journal Microbiology 48(3), 219-29.

Vespermann A, Kai M, Piechulla B. 2007. Rhizobacterial Volatiles Affect the Growth of Fungi and Arabidopsis thaliana. Applied and environmental microbiology 73, 5639-5641.

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