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Antagonistic effect of lactic acid bacteria isolated from camel milk of south Algeria against methicillin-resistant staphylococcus aureus (MRSA)

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Research Paper 10/08/2022
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Antagonistic effect of lactic acid bacteria isolated from camel milk of south Algeria against methicillin-resistant staphylococcus aureus (MRSA)

Chethouna Fatma, Boudjenah Haroun Saliha
Int. J. Biosci.21( 2), 179-188, August 2022.
Certificate: IJB 2022 [Generate Certificate]
Key: Antimicrobial activityBacteriocinLactic acid bacteriaMRSANosocomial infections

Abstract

Nosocomial infections due to methicillin-resistant Staphylococcus aureus (MRSA) are a major public health problem worldwide. The main aim of this study was to evaluate the inhibitory potential of lactic acid bacteria (LAB) against MRSA. Six strains of LAB isolated from camel milk, were identified by phenotypic method, which revealed their belonging to the species “Lc.lactis subsp.lactis”; “Lc.lactis subsp.lactis var diacetylactis”; “Lc. lactis subsp. cremoris”; “Enterococcus durans”; “Ln.mesenteroides subsp. mesenteroides”; “Lb. Plantarium”. The antimicrobial activity of these selected strains is examined against five (05) strains of methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients with infections in the intensive care unit of Slimane Amirate hospital, in the wilaya of Touggourt (Algeria) and also against two (02) control strain: methicillin-sensitive Staphylococcus aureus MSSA 25923 ATCC and Methicillin-resistant Staphylococcus aureus MRSA 43300 ATCC. Then the treatment of the culture supernatants of the isolated strains, selected antagonists “Ln. mesenteroides subsp mesenteroides” and “Lb. Plantarum” with proteolytic enzymes inactivated their inhibitory effect, indicating that the agents responsible for the inhibitions are bacteriocins. The physico-chemical characterization of bacteriocins revealed their thermo-resistance and their stability at acidic and basic pH. The results presented in this study provide a clearer idea of ​​the antibacterial potential of bacteriocins produced by Leuconostoc mesenteroides and Lactobacillus plantarum, which represent a way forward to compensate for antibiotic treatments.

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