Anthelmintic potential of powdered papaya seed Carica papaya in varying levels against Ascaridia galli in broiler chicken

Paper Details

Research Paper 10/08/2025
Views (6)
current_issue_feature_image
publication_file

Anthelmintic potential of powdered papaya seed Carica papaya in varying levels against Ascaridia galli in broiler chicken

Roniemay P. Sayson, Mylene G. Millapez, Zandro O. Perez
Int. J. Biosci. 27(2), 114-121, August 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

Parasitic infestation, particularly Ascaridia galli, significantly threatens poultry production, reducing growth and productivity. A. galli, a nematode that frequently affects chickens, can be treated with a synthetic anthelmintic; however, resistance to commercial dewormers has developed, which can cause harm to poultry. These problems catch the interest of many researchers who are driven to establish plant-based products to minimize costs. This study examines the potential anthelmintic properties of papaya seeds administered to broiler chickens at varying doses. The experiment was patterned in a Complete Randomized Design in which four treatments were observed and replicated into three, with five experimental birds in each treatment: T1- (Control), T2-(1mg PPS/bird), T3-(1.5mg PPS/bird), and T4-(2mg PPS/birds). Treatment 4, with the highest inclusion rate, has shown an excellent result, indicating that powdered papaya seed at this amount can be used as an alternative to commercial dewormers. In comparison, Treatment 3, with an amount of 1.5 mg, yields comparable results to the commercial dewormer. However, powdered papaya seed given in a lower amount yields a poorer result compared to other treatments. The findings in this study indicate that Carica papaya seed exhibits significant results against A. galli. Future studies should conduct sensory evaluations to assess the effects on the meat of broiler chickens, as well as evisceration activity, to verify the presence of gastrointestinal parasites in the gastrointestinal tract.

Acosta A. 2022. Update of the Philippine broiler industry. Veterinaria Digital, 12 December. Retrieved from https://www.veterinariadigital.com/en/articulos/update-of-the-philippine-broiler-industry/

Afolabi O, Simon-Oke I, Olasunkanmi A. 2016. Intestinal parasites of domestic chicken (Gallus gallus domesticus). Journal of Biomedicine, Akure, Nigeria.

Agricultural Training Institute. 2008. Squash production (for urban and home gardening). 2. https://ati2.da.gov.ph/ati-car/content/sites/default/files/2022-12/squash_production_guide_leaflet.pdf

Agrimate.org. n.d. Papaya farming for West Bengal. https://nhb.gov.in/Horticulture%20Crops/Papaya/Papaya1.htm

Bureau of Agricultural Research. 2018. TechnoDigest for the production of native chicken. 32.

Butcher D, Miles R. 2019. Intestinal parasites in backyard chicken flocks. https://edis.ifas.ufl.edu/publication/VM015

Catedral C, Gallego R, De Asis N, Orrillante C. 2023. Antihelmintic effect of squash seeds (Cucurbita moschata) and papaya seeds (Carica papaya) in gastrointestinal parasites in native chicken (Gallus gallus domesticus). IOP Conference Series: Earth and Environmental Science 1208(1), 012057. https://doi.org/10.1088/1755-1315/1208/1/012057

Cupo K, Beckstead R. 2019. Heterakis gallinarum, the cecal nematode of gallinaceous birds: A critical review. Avian Diseases 63(3), 381–391. https://doi.org/10.1637/0005-2086-63.3.381

Dakpogan HB, Vissiennon P. 2019. Antiparasitic activity of papaya seed extract (Carica papaya) in free-range local breed chicken (Gallus gallus) production system in Ketou. Journal of Animal & Plant Sciences 41(2). https://doi.org/10.35759/JAnmPlSci.v41-2.3

Dasenaki M, Thomaidis N. 2017. Chapter 18 – Meat safety: II residues and contaminants. In: Lawrie’s Meat Science (8th ed.), 553–583. https://doi.org/10.1016/B978-0-08-100694-8.00018-2

Debnath S, Babre N, Vyas Y. 2010. Nephroprotective evaluation of ethanolic extract of the seeds of papaya and pumpkin fruit in cisplatin-induced nephrotoxicity. https://onlinepharmacytech.info/docs/vol2issue6/JPST10-02-06-02.pdf

Department of Agriculture. 2017. Squash production guide. https://cagayanvalley.da.gov.ph/wp-content/uploads/2018/02/Squash.pdf

Department of Agriculture. 2018. Tips on poultry raising introduction. https://cagayanvalley.da.gov.ph/wp-content/uploads/2018/02/poultry1.pdf

DOST-PCAARRD. 2024. Flavors of science: How science created an authentic Ilonggo flavor-Darag native chicken.

https://www.pcaarrd.dost.gov.ph/index.php/quick-information-dispatch-qid-articles/flavors-of-science-how-science-created-an-authentic-ilonggo-flavor-darag-native-chicken

Feroza S, Ghosh AG. 2017. Effect of papaya and neem seeds on Ascaridia galli infection in broiler chicken. Pakistan Journal of Nutrition 35(1), 105–111. http://dx.doi.org/10.18681/pjn.v35.i01.p105-111

Feyera T, Rhunke I, Sharpe B. 2021. Comparative therapeutic efficacies of oral and in-water administered levamisole, piperazine and fenbendazole against experimental Ascaridia galli infection in chickens. Veterinary Parasitology 296, 109514. https://doi.org/10.1016/j.vetpar.2021.109514

Fissiha W, Kinde M. 2021. Anthelmintic resistance and its mechanism: A review. Infection and Drug Resistance 14, 3203–3211. https://doi.org/10.2147/IDR.S332378

Gerhold R. 2023. Coccidiosis in poultry. Merck Veterinary Manual. https://www.merckvetmanual.com/poultry/coccidiosis-in-poultry/coccidiosis-in-poultry

Grzybek M, Strachecka A. 2016. Evaluation of anthelmintic activity and composition of pumpkin (Cucurbita pepo L.) seed extracts—In vitro and in vivo studies. International Journal of Molecular Sciences 17(9), 1456. https://doi.org/10.3390/ijms17091456

Habtemariam S. 2019. Chapter 11 – The chemical and pharmacological basis of papaya (Carica papaya L.) as potential therapy for type-2 diabetes and associated diseases. Medicinal Foods as Potential Therapies for Type-2 Diabetes and Associated Diseases, 333–363. https://www.sciencedirect.com/science/article/abs/pii/B9780081029220000110

Hauck R. 2024. Helminthiasis in poultry. https://www.msdvetmanual.com/poultry/helminthiasis/helminthiasis-in-poultry

Jacob J. 2024. Internal parasites of poultry. https://poultry.extension.org/articles/poultry-health/internal-parasites-of-poultry/

Kettrukat T, Giersberg MF. 2023. The effect of incubation temperature on the development of the locomotory system and welfare in broiler chickens. Livestock Science 269, 105326. https://doi.org/10.1016/j.livsci.2023.105326

Kim H, Lee W, Jang H, Kang M, Kang H. 2023. The potential of non-movement behavior observation method for detection of sick broiler chickens. Journal of Animal Science and Technology. https://doi.org/10.5187/jast.2022.e105

Knott C, Lister S, Hammond P. 2009. Worms in free-range hens. https://www.thepoultrysite.com/articles/worms-in-freerange-hens

Liu M, Panda S, Luyten W. 2020. Plant-based natural products for the discovery and development of novel anthelmintics against nematodes. Biomolecules 10(3). https://doi.org/10.3390/biom10030426

Lorenzoni G. 2023. Managing chicken coccidiosis in small flocks during summer. https://extension.psu.edu/managing-chicken-coccidiosis-in-small-flocks-during-summer

Lozano J, Salinero A, Gomez L. 2019. Gastrointestinal parasites of free-range chickens- A worldwide issue. Parasites of Free-Range Chickens, 1–8. https://www.cabidigitallibrary.org/doi/pdf/10.5555/20193503968

Magdeleine C, Mahieu M, Archimède H. 2011. Chapter 110- Pumpkin (Cucurbita moschata Duchesne ex Poir.) seeds as an anthelmintic agent? In: Nuts and Seeds in Health and Disease Prevention. https://www.sciencedirect.com/science/article/abs/pii/B9780123756886101100

Men X, Choi S, Kwon H, Han X. 2020. Physicochemical, nutritional and functional properties of Cucurbita moschata. https://pmc.ncbi.nlm.nih.gov/articles/PMC7914307/

Muhamad SA. 2017. The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed. International Food Research Journal.

Nur K. 2021. Organoleptic qualities of broiler chicken meat given with herbal feed with turmeric. Chalaza Journal of Animal Husbandry.

Peralta R. 2015. Ground squash seeds as potential alternative dewormer for native chicken. https://ejournals.ph/article.php?id=11496

PhilAtlas. 2020. Talo-ot, Argao, Cebu Profile. https://www.philatlas.com/visayas/r07/cebu/argao/talo-ot.html

Prastowo JH. 2017. Effects of Areca catechu seed and Anredera cordifolia leaf on Ascaridia galli infection in the domestic chicken (Gallus gallus domesticus).

Regmi P. 2024. Internal parasites in free-range chicken farming: Roundworms. https://modernpoultry.media/internal-parasites-in-free-range-chicken-farming-roundworms/?mp=1732434831115

Roshan A. 2014. A brief study on Carica papaya– A review. https://www.researchgate.net/publication/307904829_A_Brief_Study_on_Carica_Papaya-_A_Review

Ruhnke INB. 2017. Immune responses following experimental infection with Ascaridia galli and necrotic enteritis in broiler chickens. Avian Pathology 46(5), 523–532. https://doi.org/10.1080/03079457.2017.1330536

Salvedia C. 2023. Efficacy of Carica papaya, Areca catechu, and Manihot esculenta extracts against Ascaridia galli eggs (in vitro). https://www.cabidigitallibrary.org/doi/pdf/10.5555/20230373346

Shifaw A, Feyera T, Sharpe B. 2023. Prevalence and magnitude of gastrointestinal helminth infections in cage-free laying chickens in Australia. One Health 16, 100479. https://www.sciencedirect.com/science/article/abs/pii/S2405939022001356

Singh K, Sharma P, Gaur A, Parihar H. 2022. Anthelmintic activity of aqueous and alcoholic extracts of Carica papaya seeds in naturally infested goats. Asian Journal of Dairy and Food Research 41(4). https://doi.org/10.18805/ajdfr.DR-1964

Sugiharto S. 2020. Papaya (Carica papaya L.) seed as a potent functional feedstuff for poultry- A review. Veterinary World 13(8), 1613–1619. https://doi.org/10.14202/vetworld.2020.1613-1619

University of the Philippines Los Baños (UPLB). 2019. Housing for broiler production – AMTEC. https://amtec.uplb.edu.ph/wp-content/uploads/2019/07/402.pdf

WebMD Editorial Contributor. 2022. Health benefits of squash. https://www.webmd.com/diet/health-benefits-squash

World Organisation for Animal Health. 2021. Responsible and prudent use of anthelmintic chemicals to help control anthelmintic resistance to grazing livestock species. Paris.

Wu Z, Fang H, Xu Z, Lian J. 2022. Molecular characterization analysis of prevalent infectious bronchitis virus and pathogenicity assessment of recombination strain in China. Frontiers in Veterinary Science 9, 842179. https://doi.org/10.3389/fvets.2022.842179

Ybanez R, Resuelo K, Kintanar A, Ybanez A. 2018. Detection of gastrointestinal parasites in small-scale poultry layer farms in Leyte, Philippines. Veterinary World 11(11), 1587–1591. https://doi.org/10.14202/vetworld.2018.1587-1591

Yebes A. 2021. Papaya production guide. 1–8. https://www.buplant.da.gov.ph/images/Production_guide/pdf/Papaya%20.pdf

Zirintunda G, Biryomumaisho S, Kasozi K, Batiha G. 2022. Emerging anthelmintic resistance in poultry: Can ethnopharmacological approaches offer a solution? Frontiers in Pharmacology 12, 774896. https://doi.org/10.3389/fphar.2021.774896

Related Articles

Tumor suppressing ability of myrtenal in DMBA-induced rat mammary cancer: A biochemical and histopathological evaluation

Manoharan Pethanasamy, Shanmugam M. Sivasankaran, Saravanan Surya, Raju Kowsalya, Int. J. Biosci. 27(2), 141-150, August 2025.

Assessing tree diversity in cashew plantations: Environmental and agronomic determinants in buffer zones of Mont Sangbé National Park, western Côte d’Ivoire

Kouamé Christophe Koffi, Kouakou Hilaire Bohoussou, Serge Cherry Piba, Naomie Ouffoue, Sylvestre Gagbe, Alex Beda, Adama Tondossama, Int. J. Biosci. 27(2), 122-133, August 2025.

Valorization of fish scale waste for the synthesis of functional gelatin-based biopolymers

N. Natarajan Arun Nagendran, B. Balakrishnan Rajalakshmi, C. Chellapandi Balachandran, Jayabalan Viji, Int. J. Biosci. 27(2), 102-113, August 2025.

Isolation and characterization of phosphate solubilising bacteria undernath Excoecaria agallocha L. of muthupet mangrove reserve

Ms. S. Alice Keerthana, V. Shanmugaraju, P. Arun, M. Poongothai, Int. J. Biosci. 27(2), 83-89, August 2025.

Hematological and biochemical parameters in mono- and associative invasions of domestic chickens by helminths and eimeria in Azerbaijan

Aygun A. Azizova, Ramin S. Mammadov, Ugur Uslu, Int. J. Biosci. 27(2), 76-82, August 2025.

Evaluation of phytocompounds from Azima tetracantha using UV-VIS and FTIR analysis

R. Devi Anbarasi, V. Ramamurthy, Int. J. Biosci. 27(2), 69-75, August 2025.