Anti-angiogenic and non-cytotoxic potentials of aqueous and acetone extracts of the stem of Philippine Forest Liana, Bauhinia integrifolia Roxb
Paper Details
Anti-angiogenic and non-cytotoxic potentials of aqueous and acetone extracts of the stem of Philippine Forest Liana, Bauhinia integrifolia Roxb
Abstract
The decoction of Bauhinia integrifolia stem is recommended by Manobo tribe herbalists to treat relapse that are experienced by most mothers after child birth while some used it as abortifacient and birth control. To test its anti-angiogenic and cytotoxic potentials, the bioactive compounds in the stem of B. integrifolia were extracted using 70% acetone and water through decoction. The anti-angiogenic activity of the extracts was assessed using duck chorion-allatoic membrane. The result of the assay showed that both acetone and aqueous extracts exhibited dose dependent anti-angiogenic activity. As the extracts’ concentration increased from 10 mg ml-1 to 1000 mg ml-1, the formation of new blood vessels decreased. However, acetonic extracts showed significantly more lower blood vessel formation compared to the decoction. The evaluation of the cytotoxic activity of the stem extracts employing brine shrimp lethality assay, showed that both extracts are non- cytotoxic since the acetonic extract has LC50 of 9,444 µg ml-1 and the nauplii survived in all concentrations of aqueous extract. With the above results, it is concluded that the extract of B. Integrifolia is non-cytotoxic anti-angiogenic agent. The isolation and further test of the active component possessed by this plant are recommended.
Eloff JN. 1998. Which extract should be used for the screening and isolation of antimicrobial compounds from plants? Journal of Ethnopharmacology 60, 1-8.
Guevara BQ (Ed). 2005. A guidebook to plant screening: Phytochemical and biological. Manila, Philippines: University of Santo Tomas Research Center for the Natural Sciences, 135-136.
Gupta MP, Monge A, Karitas G, Lopez de Cerain A, Solis PN, Leon E, de Trujillo M, Surez O, Wilson F, Montenegro G, Noriega Y, Santana AI, Correa M, Sanchez C. 1996. Screening of Panamanian medicinal plants for brine shrimp toxicity, crown gall tumour inhibition, cytotoxicity and DNA interaction. International Journal of Pharmacology 34, 123-127.
Jocano FL. 1970. Maternal and child care among the Tagalogs in Bay, Laguna, Philippines. Asian Studies Journal, 277 – 300.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: convenient general bioassay for active plant constituents. Plant Medicine 45, 31-34.
Oklu R, Walker TG, Wicky S, Hesketh R. 2010. Angiogenesis and current anti-angiogenic strategies for the treatment of cancer. Journal of Vascular Intervention. Radiology 21(12), 1791-1805. http://dx.doi.org/10.1016/j.jvir.2010.08.009.
Pagulayan AJ, Cauyan GA, Quinto EA Madulid RS. 2006. The Philippine Biota: The brine shrimp bioassay (BSB). Manila: Department of Biological Sciences, University of Santo Tomas, 23-32.
Savithramma N, Linga Rao M, Bhumi G. 2011. Phytochemical screening of Thespesia populnea (L.) Soland and Tridax procumbens L. Journal of Chemical and Pharmaceutical Research 3(5), 28-34.
Sreeshma LS, Nair BR. 2014. Brine shrimp lethality in two species of Biophytum DC. (Oxalidaceae). International Journal of Pharmacy and Pharmaceutical Sciences 6(4), 582-586.
Leila A. Allado-Ombat, Franco G. Teves, 2015. Anti-angiogenic and non-cytotoxic potentials of aqueous and acetone extracts of the stem of Philippine Forest Liana, Bauhinia integrifolia Roxb. Int. J. Biosci., 7(5), 184-191.
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