Anti-oxidant and Immunomodulatory Effects of newlectins extracted from Algerian plants: Ephedra alata, Zizyphus jujuba and Calycotome villosa
Paper Details
Anti-oxidant and Immunomodulatory Effects of newlectins extracted from Algerian plants: Ephedra alata, Zizyphus jujuba and Calycotome villosa
Abstract
The lectins present in plants of Ephedra alata, Zizyphus jujuba and Calycotome villosa were extracted by soluble proteins (crude extract) in phosphate buffer (0.1M, pH 7.2). Peptide fraction and purified peptide of Ephedra alata, Zizyphus jujube and Calycotome villosa showed the highest anti-oxidant activity in the DPPH, SOD and Ferric reducing assay radical scavenging model. Extracted lectin of Ephedra alata, Zizyphus jujubaand Calycotome villosa showed the thermostability is more than 100°C. However, the lectin of Ephedra alata, Zizyphus jujub aand Calycotome villosa was stable in the pH ranging between 3-12, 2- 12 and 1- 12respectively and were not inhibited by the sugars. Immunomodulatory activitiesof extracted lectins from Ephedra alata, Zizyphus jujube and Calycotome villosa were evaluated on phagocytic activity by carbon clearance test. Adult Albinos wistar mice randomly divided into three groups were the first served as a control, while the remaining groups respectively treated with extracted lectins from plants of Ephedra alata, Zizyphus jujubaand Calycotome villosa at the dose of 25 and 100 mg/kg by intraperitoneal injection (IP). Change in phagocytic activity was determined after 48 h injection of carbon ink suspension. In carbone clearance test, extracted lectins from Ephedra alata, Zizyphus jujube and Calycotome villosa exhibited significantly phagocytic index dose-dependent against the control group, indicating stimulation of the reticulo-endothelial system. The present study thus reveals that extracted lectins from Ephedra alata, Zizyphus jujuba and Calycotome villosa holds promise as an immunomodulatory agent, which acts by stimulating dose-dependent phagocytic function.
Bradford MA. 1976. Anal Biochemestry 72, 248-54.
Elias RJ, Kellerby SS, Decker EA. 2008. Critical Review in Food Science and nutrition 48, 430–441.
Carlini CR, Grossi-de-Sa MF. 2002. Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides, Toxicology 40 (11), 1515–1539.
Cheng W, Li J, You T, Hu C. 2005. Anti-inflammatory and immunomodulatory activities of the extracts from the inflorescence of Chrysanthemum indicum Linné. Journal of Ethnopharmacology 101(1-3), 334-7.
Cole MD. 1994. Key antifungal, antibacterial and antiinsect assays – a critical review. Biochemical System Ecology 22, 837–856.
Correia MTS, Coelho LCBB. 1995. Purification of a glucose/mannose specific lectin, isoform1, form seeds of cratylia mollis mart. (Camaratu bean). Applied Biochemical Biotechnology 55(3), 261-273.
Cumming RD. 1997. Lectins as tools for glycoconjugate purification and characterization. In Glyco-science, status and perspectives. (H.J. Gabius & S. Gabius, eds.) Champman & Hall GmbH, Weinheim, p 191-199.
Deeksha MK, Sangha DS, Khurana MB, Binay S. 2015. Screening for lectin quantification in Brassica Spp and vegetable crops. Journal of environmental and Applied Bioresearch 30 (1), 20-24. Duan X et al., Molecules, 2007 12(4), 759-771.
Goldstein IJ, Hughes RC, Monsigny M, Ozawa T & Sharon N. 1980. What should be called a lectin? Nature 285, 60.
Hamshou M, Smagghe G, Van Damme EJM. 2010. Entomo-toxic effects of fungal lectin rhizoctonia solani towards spondoptera littoralis. Fungal Biology 114(1), 34-40.
Harmsma M, Gromme M, Ummelen M, Dignef W, KJ Tusenius. 2004. International journal of oncology 125, 1521-1529.
Irobi ON, Moo-Young M, Anderson WA. 1996. Antimicrobial Activity of Annatto (Bixa orellana) Extract. 34(2), 87-90.
Lis H, Sharon N. 1981. Lectins in higher plants. Biochemistry of Plants 6, 371-447.
Michiels K, Van Damme EJM, Smagghe G. 2010. Plant-insect interactions: what can we learn from plant lectins? Arch Insect Biochemical Physiology 73(4), 193–212.
Necib Y, Bahi A, Merouane F, Bouadi H, Boulahrouf K. 2014. Comparative study of a new lectin extracted from roots of plants: Cyperus rotundus, Pistacia lentiscus and Ruta graveolens.World Journal of pharmacology research 4(1), 1720-1733.
Necib Y, Bahi A, Derri N, Merouane F, Bouadi H and Boulahrouf K. 2014. Immunomodulatory of lectin extracted from roots of blak mulberry (Morus Nigra).World Journal of pharmacology research, 4(1), 1707-1719.
Necib Y, Bahi A, Zerizer S, Cherif A, Boulakoud MS. 2013. Immunomodulatory Activity of Argan oil (Argania Spinosa. L). Intertional Journal of Pharmacology Sciences Review and Research 23(1), 57-59.
Necib Y, Bahi A, Zerizer S. 2013. Immunomodulatory Effect of Argan oil (Argania spinosa. L) After Exposure to Mercuric Chloride in Mice. International Journal of Pharmacology Sciences Review and Research 23(1), 191-193.
Peumans WJ, Van Damme WJN. 1995. Lectin as plant defense proteins. Plant Physiology 109, 347-352.
Pusztai A, Bardocz S. 2009. Biological effects of plant lectins on the gastrointestinal tract: metabolic consequences and applications. Trends Glycosciences Glycotechnology 8(41), 149–165.
Shah AS, Wakade AS, Juvekar AR. 2008. Immunomodulatory activity of methanolic extract of Murraya koenigii (L) Spreng leaves. Indian Journal of Experimental Biology 46(7), 505-9.
Shaista R, Sakeena Q, Ishfak HW, Showkat AG, Akbar M, Rabia H. 2014. Purification and partial characterization of a Fructose-binding lectin from the leaves of Euphorbia helioscopia. Pakistan Journal of Pharmacology Sciences 27(6), 1805-1810.
Souza ME, Pinto MM. 2005. Síntesis of xanthones: an overview. Curr Med Chem 12, 2447–2479.
Van Damme EJM, Lannoo N, Peumans WJ. 2008. Plant lectins. Advance Botany Research 48, 109–209. http://dx.doi.org/10.1016/50065-2296(08)00403-5.
Ahlem Bahi, Youcef Necib, Fateh Merouane, 2022. Anti-oxidant and Immunomodulatory Effects of newlectins extracted from Algerian plants: Ephedra alata, Zizyphus jujuba and Calycotome villosa. Int. J. Biosci., 20(2), 69-81.
Copyright © 2022 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.