Antibacterial activity of important medicinal plants on human pathogenic bacteria

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Research Paper 01/06/2015
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Antibacterial activity of important medicinal plants on human pathogenic bacteria

Khaista Rahman, Muhammad Nisar, Amin Ullah Jan, Muhammad Suliman, Ajmal Iqbal, Ayaz Ahmad, Rukhsana Ghaffar
Int. J. Agron. Agri. Res.6( 6), 106-111, June 2015.
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Abstract

The resistance of pathogenic bacterial strains to antibiotics is the major burning issue around the world. To minimize the strain resistance to antibiotic local medicinal plant were selected. The leaves of medicinal plants (Ajuga bracteosa Benth, Calotropis procera and Zizyphus sativa Geartn) were collected and antibacterial activity was examined through agar well diffusion method. The crude extracts were obtained by using methanol and n-hexane as the extraction solvent. Three concentrations (100 mg/ml, 50 mg/ml, and 25 mg/ml) were used to check the antibacterial activity of plant extracts. Each plant sample was tested against two Gram-positive (Bacillus cereus, Bacillus subtilis) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi) bacteria. The methanol extracts showed better activity against all the tested bacterial strains, except Zizyphus sativa Geartn showed inactive against Pseudomonas aeruginosa and Salmonella typhi. The n-hexane extracts showed moderate activity against all the bacterial strains, whereas the n-hexane extract of Zizyphus sativa Geartn were inactive against Salmonella typhi and Bacillus cereus. It is concluded that methanol extract showed good antibacterial activity as compared to n-hexane extract and further study is needed to check the activity of the tested plants against the other bacterial strains.

VIEWS 5

Adzu B, Amos S, Wambebe C, Gamaniel K. 2001. Antinociceptive activity of Ziziphusspina-christi root bark extract. Fitoterapia 72, 334-350.

Ali SI, Nasir YJ. 1990. Flora of Pakistan “Department of Botany, University of Karachi, Pakistan 192, P.235.

Ali SI, Qaiser M. 2004. Flora of Pakistan, Department of Botany, University of Karachi, Pakistan.

Bajpai M, Pande A, Tewari SK, Prakash D. 2005. Phenolic contents and antioxidant activity of some food and medicinal plants. International Journal of Food Sciences and Nutrition 56(4), 287-291.

Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. 1985. Natural plant chemicals: Sources of Industrial and Medicinal materials, Science 228, 1154-1160.

Cherry M. 1985. The needs of the people. In Wickens, G. E., Goodin .J.R., Field DV (eds). Plants for arid lands. Unwin Hyman, London 48-49.

Cyrus WG, Daniel GW, Nanyingi MO, Njonge FK, Mbaria JM. 2008. Antibacterial and cytotoxic activity of Kenyan medicinal plants, MemInst Oswaldo Cruz, Rio de Janeiro 103(7), 650-652.

Jain SK. 1994. Ethno botany and research on medicinal plants in India. Ciba Found Sym 185, 153– 64.

Kareem SO, Akpan I, Ojo OP. 2008. Antimicrobial Activities of Calotropis procera on Selected Pathogenic Microorganisms, African Journal of Biomedical Research, 11, 45-51.

Klein Schmidt HE, Johnson RW, 1977.Weeds of Queensland. 147 S. Zitiertnach PIER.

Kumar VL, Arya S. 2006. Medicinal uses and pharmacological properties of Calotropis procera. In: Recent Progress in Medicinal Plants, Ed., Govil, J.N. Studium Press, Houston, Texas, USA 373-388.

Mahesh B, Satish S. 2008. Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens, World Journal of Agricultural Sciences 4, 839-843.

Malik F, Hussain S, Mirza T, Hameed A, Ahmad S, Riaz R, Shah PA, Usmanghani K. 2011. Screening for antimicrobial activity of thirty-three medicinal plants used in the traditional system of medicine in Pakistan. Journal of Medicinal Plant Reserch 5(14), 3052-3060,

Nasir E, Ali SI. 1971-95. Flora of Pakistan. Nos. 1-190. Department of Botany, University of Karachi. Pakistan Agriculture Research. Council Islamabad, Pakistan.

Nasrullah, Suliman, Rahman K, Ikram M, Nisar M, Khan I. 2012. Screening of Antibacterial Activity of Medicinal Plants. International Journal of Pharmaceutical Science Review and Reserch 14(2), 25‐29.

Perry LM, Metzger J. 1980. Medicinal Plants of East and Southeast Asia. The MIT Press Cambridge, London 184.

Stewart RR. 1967. Checklist of plants Swat state, Northwest Pakistan. Pakistan Journal of Forestry 4(2), 457 528.

Stewart RR. 1982. History and exploration of plants in Pakistan and adjoining areas, National Herbarium, NARC, Islamabad.

Walter C, Shinwari Z K, Afzal I and Malik RN. 2011. Antibacterial Activity in Herbal Products Used In Pakistan. Pakistan Journal of Botany 43, 155-162.

Wojdylo A, Oszmianski J, Czemerys R. 2007 Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry 105, 940-949.