Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | May 1, 2014

VIEWS 1
| Download 2

Antibacterial activity of silver nanoparticles produced by Plantago ovata seed extract against antibiotic resistant Klebsiella pneumoniae

Mohammad Bokaeian, Taher Mohasseli, Saeide Saeidi, Nahid Sephri

Key Words:


J. Bio. Env. Sci.4(5), 125-131, May 2014

Certification:

JBES 2014 [Generate Certificate]

Abstract

The synthesis of nanoparticles has become the matter of great interest in recent years due to its various advantageous properties and applications in various fields. Though physical and chemical methods are more popular for nanoparticle synthesis, the biogenic production is a better option due to eco-friendliness.The purpose of this study is to synthesis of silver nanoparticles by using green method on extract from Plantago ovata and determine its potential antibacterial effects against antibiotic resistant Klebsiella pneumoniae isolates.A total of 30 K.pneumoniae strains were isolated from urine cultures of hospitalized patients suffering from urinary tract infections in three hospitals in Zahedan during the years 2011- 2012. Isolated bacteria were identified by Gram’s stain and standard biochemical tests. The susceptibility of used antibiotics was carried out using standard disc diffusion method. The seeds of Plantago ovata were used for silver nanoparticle sunthesis. UV–vis spectral and Transmission Electron Microscopy analysis were used in order to confirm the formation of silver nanoparticles. The broth micro-dilution method was used to determine MIC of silver nanoparticles. The antibiotic resistance profile of K. pneumoniae isolates was as follow: Penicillin (93.3%), Erythromycin and Ampicillin (76.6%), Tetracycline and Cefixime (53.3%), Ceftazidime (40%) and Nalidixic acid (36.6%). The highest and the least MIC of P. ovate seed extract values were found to be 200 and 12.5 ppm respectively. The present study concludes that at a specific dose, chitosan-based AgNPs kill bacteria without harming the host cells, thus representing a potential template for the design of antibacterial agents to decrease bacterial colonization and to overcome the problem of drug resistance.

VIEWS 1

Copyright © 2014
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Antibacterial activity of silver nanoparticles produced by Plantago ovata seed extract against antibiotic resistant Klebsiella pneumoniae

Sotiriou GA, Pratsinis SE. 2010. Antibacterial Activity of Nanosilver Ions and Particles. Environmental Science & Technology 44, 5649-5654. http://dx.doi.org/ 10.1021/es101072s

Sun RW, Chen R, Chung NP, Ho CM, Lin CL, Che CM. 2005. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chemical Communications 40, 5059–5061. http://dx.doi.org/ 10.1039/b510984a

Dibrov P, Dzioba J, Gosink KK, Häse CC. 2002. Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholerae. Antimicrobial Agents and Chemother apy 46, 2668–2670. http://dx.doi.org/10.1128/AAC.46.8.2668-2670.2002

Ahearn DG, May LL, Gabriel MM. 1995. Adherence of organisms to silver-coated surfaces. Journal of Industrial Microbiology 15, 372–376. http://dx.doi.org/ 10.1007/BF01569993

Ghandour W, Hubbard JA, Deistung J, HughesMN, Poole RK. 1988. The uptake of silver ions by Escherichia coli K12: toxic effects and interaction with copper ion. Applied Microbiology and Biotechnology 28, 559–565.

Sharma VK, Yngard RA, Lin Y. 2009. Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science 145, 83–96. http://dx.doi.org/10.1016/j.cis.2008.09.002

Salas-Salvado J, Farres X, Luque X, Narejos S, Borren M, Blanza R. 2007. Effect of two doses of a mixture of soluble fibers on body weight and metabolic variables in over-weight or obese patients: A randomized trial. British Journal of Nutrition 99, 1380-1387. http://dx.doi.org/10.1017/S0007114507868528

Washington N, Harris M, Mussellwithe A, Spiller RC. 1999. Moderation of lactulose–induced diarrhea by Psyllium: Effects on motility and fermentation. The American Journal of Clinical Nutrition 67, 317-321.

Hannan JMA, Ali L, Khaleqe J, Akhter M, Flatt PR, Abdel-Wahab YHA. 2006. Aqueous extracts of husks of Plantago ovate reduce hyperglycaemia glucose absorption. British Journal of Nutrition 96,131-137. http://dx.doi.org/10.1079/BJN20061819

Souri E, Amin G, Farsam H, Tehrani MB. 2008. Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts. DARU 16, 83-87.

Forbes  BA,  Sahm  DF,  Weissfeld  AS.  2007. Bailey  &  Scott`s  diagnostic  microbiology.  12th  ed. Missouri: Mosby Co; 323-333.

Manikprabhu D, Lingappa K. 2013. Microwave assisted rapid and green synthesis of silver nanoparticles using a pigment produced by Streptomyces coelicolor KImp 33. Bioinorganic Chemistry and Applications 2013:341798, 1-8.

Saeidi S, Alavi-Naini R, Shayan S. 2014. Antimicrobial susceptibility and distribution of TEM and CTX-M genes among ESBL-producing Klebsiella pneumoniae and Pseudomonas aeruginosa causing urinary tract infection. Zahedan Journal of Research in Medical Sciences 16(4), 1-5.

Zamani A, Yousefi Mashouf R, Ebrahimzadeh NamvarA, Alikhani M.Y. 2013. Detection of magA Gene in Klebsiella spp. Isolated from Clinical Samples. Iranian Journal of Basic Medical Sciences 16, 173-76.

Sikarwar SA and Batra HV. 2011. Prevalence of Antimicrobial Drug Resistance of Klebsiella pneumoniae in India. International Journal of Bioscience, Biochemistry and Bioinformatics 3(1).

Feizabadi MM, Etemadi G, Rahmati M, Mohammadi-Yeganeh S, Shabanpoor S, Asadi S. 2007. Antibiotic Resistance Patterns and Genetic Analysis of Klebsiella Pneumoniae Isolates from the Respiratory Tract. National Research Institute of Tuberculosis and Lung Disease. Journal of Respiratory Disease, Thoracic Surgery, Intensive care and Tuberculosis 6(3), 20-25.

Soo-Hwan K, Lee HS, Ryu DS, Choi SG, and Lee DS. 2011. Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli. Korean Journal of Microbiology and Biotechnology 39(1), 77–85.

Sondi I, Salopek-Sondi B. 2004. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275, 177–182. http://dx.doi.org/10.1016/j.jcis.2004.02.012

Guzman M, Dille J, Godet S. 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. International Journal of Chemical and Biomolecular Engineering 2(3), 104- 111.

Benn T, Westerhoff P. 2008. Nanoparticle silver released into water from commercially available sock fabrics. Environmental Science & Technology 42, 4133–4139. http://dx.doi.org/10.1021/es7032718

Chen C, Chiang C. 2008. Preparation of cotton fibers with antibacterial silver nanoparticles. Journal of Materials Science Letters 62, 3607–3609. http://dx.doi.org/ 10.1016/j.matlet.2008.04.008

Falletta E, Bonini M, Fratini E, Lo Nostro A, Pesavento G, Becheri A, Lo Nostro P, Canton P, Baglioni P. 2008. Clusters of poly (acrylates) and silver nanoparticles: structure and applications for antimicrobial fabrics. The Journal of Physical Chemistry 112, 11758–11766. http://dx.doi.org/ 10.1021/jp8035814

Hernandez-Sierra J, Ruiz F, Pena D, Martinez-Gutierrez F, Martinez A, Guillen A, Tapia-Perez H, Castanon G. 2008. The antimicrobial sensitivity of Streptococcus mutants to nanoparticles of silver, zinc oxide, and gold. Nanomed Nanotechnology. 4,237–240. http://dx.doi.org/ 10.1016/j.nano.2008.04.005

Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. 2008. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Dekker Encyclopedia of Nanoscience and Nanotechnology 4, 141–144. http://dx.doi.org/ 10.2174/157341308784340804

Jung W, Koo H, Kim K, Shin S, Kim S, Park Y. 2008. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Applied and Environmental Micro-biology 74,2171–2178. http://dx.doi.org/ 10.1128/AEM.02001-07

Kim J. 2007. Antibacterial activity of Ag+ ion-containing silver nanoparticles prepared using the alcohol reduction method. Journal of Industrial and Engineering Chemistry 13,718–722.

Kim Y, Kim J, Cho H, Rha D, Kim J, Park J, Choi B, Lim R, Chang H, Chung Y, Kwon I, Jeong J, Han B, Yu I. 2008.Twenty-eight-day oral toxicity, genotoxicity, and gender related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 20, 575–583. http://dx.doi.org/ 10.1080/08958370701874663

Kim K, Sung W, Moon S, Choi J, Kim J, Lee D. 2008. Antifungal effect of silver nanoparticles on dermatophytes. Journal of Microbiology and Biotec-hnology 18, 1482–1484.

Kvitek L, Panacek A, Soukupova J, Kolar M, Vecerova R, Prucek R, Holecova M, Zboril R. 2008. Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs). The Journal of Physical Chemistry 112, 5825–34.

Gopinath K, Gowri S, Arumugam A. 2013. Phytosynthesis of silver nanoparticles using Pterocarpus santalinus leaf extract and their antibacterial properties. Journal of Nanostructure in Chemistry 3, 63- 68. http://dx.doi.org/ 10.1186/2193-8865-3-68

Das R, Gang S, Nath SS. 2011. Preparation and Antibacterial Activity of Silver Nanoparticles. Journal of Biomaterials and Nanobiotechnology 2, 472-475.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background