Antibacterial potential of indigenous red mangrove (Rhizophora racemosa) fungal endophytes and bioactive compounds identification
Paper Details
Antibacterial potential of indigenous red mangrove (Rhizophora racemosa) fungal endophytes and bioactive compounds identification
Abstract
The antibacterial activity of indigenous red mangrove leaves- endophytic fungi against marine pathogenic bacteria and bioactive compounds identification and quantification were carried out. Healthy leaves of red mangrove (Rhizophora racemosa) were collected from Eagle Island mangrove forest in Port Harcourt, Nigeria. The endophytic fungi were isolated with acidified Potato dextrose agar plates after incubation at 28°C for 5 days. Isolated colonies were identified based on their colonial and microscopic morphology. The ethyl acetate and methanolic extracts of the mangrove leaves-endophytic fungal isolates were screened against marine pathogenic bacteria (Staphylococcus aureus, Salmonella sp., Shigella sp., Vibrio cholerae and Vibrio parahaemolyticus) using agar well diffusion assay at different concentrations. An isolate which was active against all the pathogens was further identified molecularly and the bioactive compounds in its methanolic extract identified and quantified using Gas chromatography – Mass spectrometry analysis. Six endophytic fungi of the genera Rhizopus, Pestalotiopsis, Aspergillus, Penicillium, Geotrichum and Phomopsis were isolated. Methanolic extract of Aspergillus sp., identified molecularly as Aspergillus niger KU350621.1, was active against all the tested pathogens. Eleven bioactive compounds were identified in its methanolic extract with 9, 12-Octadecadienoic acid (Z, Z) methyl ester (C19H34O2) as the major compound at 39.896% peak area. These results reveal that red mangrove leaves- endophytic fungi have antibacterial effect against marine pathogenic bacteria. The identified bioactive compounds can be used for drug development.
Abhiroop C, Pranabes S, Subodh KM. 2014. Ethnobotanical understanding of Mangrove. Interna-tional Journal of Botany and Research 4, 29-34.
Alias SA, Zainuddin N, Lee CW, Ebel R, Othman NA, Mukhtar MR, Awang K. 2010. Antimicrobial activities of marine fungi from Malaysia. Botanica Marina 53, 507-513.
Alvin A, Miller KI, Neilan BA. 2014. Exploring the potential of Endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiology Research 169, 483-495.
Ariole CN, Ihunwo HO, Ozuru CN. 2014. Isolation of Marine Green Alga – Associated Microorganisms and their Antimicrobial activity against Fish Pathogenic Aeromonas Strain. Journal of Chemical,Biological and Physical Sciences 4(4), 3279-3286.
Bhimba BV, Agnel DA, Mathew JM, Jose GM, Joel EL, Thangaraj M. 2012. Anticancer and antimicrobial activity of Mangrove derived fungi Hypocrea lixii. Chinese Journal of Natural Medicines 10, 77-80.
Chaeprasert S, Piapukiew AJ, Sihanonth P. 2010. Endophytic fungi from Mangrove plant species of Thailand: their antimicrobial and anticancer potentials. Botanica Marina 53, 555-564.
Gehan MA, Hanan AE, Hassan AHI, Okbah MA. 2009. Marine natural products and their potential applications as anti-infective agents. World Sciences Journal 7(7), 872-880.
Huang H, Feng X, Xiao Z, Liu L, Li H, Ma L. 2008. Azaphilones and p-terphenyls from the mangrove endophytic fungus Penicillium chermesinum (ZH4-E2) isolated from the South China Sea. Journal of Natural Products 74, 997-1002.
Jirayu B, Soulwalak P, Vatcharin R, Juriya S. 2011. Antimicrobial activity of Crude extracts from Mangrove Fungal Endophytes. World Journal of Microbiology Biotechnology 27, 3005-3008.
Joseph B, Priya RM. 2010. Preliminary phytochemicals of Psidum guajava (L) leaf of methanol extract and its cytotoxic study on HeLa cell lines. Inventi cytotrophic. 87, 10.
Kjer J, Debbab A, Amal HA, Proksch P. 2010. Methods for isolation of marine derived endophytic fungi and their bioactive secondary products. Nature Protocol 5(3), 479-490.
Kumaresan V, Suryanarayanan TS. 2008. Occur-rence and distribution of endophytic fungi in a mang-rove community. Mycology Research 105, 1388-1391.
Liu K, Ding X, Deng B, Chen W. 2007. Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis. Journal of industrial Microbiology Biotechnology 36, 1171–1177.
Maria GL, Sridhar KR, Raviraja NS. 2005. Antimicrobial and enzyme Activity of mangrove endophytic fungi of South west coast of India. Journal of Agricultural Technology 1, 67-80.
Richardson AE, Barea JM, McNeill AM, Prigent-Combaret C. 2009. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321, 305-339.
Schulz B, Boyle C, Draeger S, Römmert AK. 2006. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycological Research 106, 996-004.
Silva MRO, Almeida AC, Arruda VF, Gusmao N. 2011. Endophytic Fungi from Brazilian mangrove plant Laguncularia racemosa: their antimicrobial potential. In: A Mendez-Vilas, editor. Science against microbial pathogens. Communicating Current Research and Technological Advances 2, 1260-1266.
Strobel GA, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Molecular biology review 67(4), 491-502.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.
Tao MH, Li DL, Zhang WM, Tan JW, Wei XY. 2011. Study on the Chemical constituents of endophytic fungus Fimetariella rabenhorstii Isolated from Aquilaria sinensis. Journal of Chinese Medicinal Materials 34, 221-223.
Yang J, Xu F, Huang C, Li J, She Z, Pei Z, Lin Y. 2010. Metabolites from the mangrove endophytic fungus Phomopsis sp. (H76). European Journal of Organic Chemistry 5, 3692-3695.
Zhang YJ, Zhang S, Liu XZ, Wen HA, Wang M. 2010. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in Applied Microbiology 51, 114-118.
C. N. Ariole, A. E. Akinduyite (2016), Antibacterial potential of indigenous red mangrove (Rhizophora racemosa) fungal endophytes and bioactive compounds identification; IJMM, V4, N4, November, P14-24
https://innspub.net/antibacterial-potential-of-indigenous-red-mangrove-rhizophora-racemosa-fungal-endophytes-and-bioactive-compounds-identification/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0