Antibacterial potential of the crude extracts of Trianthema portulacastrum L.
Paper Details
Antibacterial potential of the crude extracts of Trianthema portulacastrum L.
Abstract
The weed indigenous to Punjab belonging to family Aizoaceae namely Trianthema portulacastrum was assessed for its antibacterial perspective. The steady-state maceration used for the extract preparation established that more phytochemical contents were macerated in fruit extract. The antibacterial potential was assessed employing agar well diffusion technique for measuring zone of inhibition and verified using agar dilution scheme by analyzing Minimum Inhibitory Concentration. Antibacterial activity ranged from 13.4±0.90 to 35.9±0.46mm, with maximum potential reported by ethanol extract of stem and minimum efficacy obtained by alcoholic macerate of root against E. coli. In addition, significant MIC (1.25mg/mL) was exhibited by ethanol extract of fruit against B. subtilis, ethanol extract of stem and n-hexane extract of fruit in opposition to E. coli, chloroform extract of fruit in contradiction to P. aeruginosa and ethanol extract of fruit against S. aureus. The correlation between zones of inhibition and MIC values concluded that negative association exist between the two categories i.e. increase in the zones of inhibition lead to the decrease in MIC and vice versa.The preliminary results presented in this study put forward some potential macerates derived from T. portulacastrum. However, further studies are needed to identify and isolate the active compounds responsible for the antibacterial action.
Abdullah N, Syida WS, KamarudinW, Samicho Z, Aziman N, Zulkifli KS. 2012. Evaluation of in vitro antioxidant and antimicrobial activities of the various parts of Benincasa hispida. International Journal of Pharm Tech Research 4(4), 1367-1376.
Anwar F, Ali M, Hussain AI, Shahid M. 2009. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal 24, 170-176. http://dx.doi.org/10.1002/ffj.1929
Baloch N, Nabi S, Al-Kahraman YMSA. 2013. In vitro antimicrobial, insecticidal, antitumor activities and their phytochemical estimation of methanolic extract and its fractions of Medicago lupulina leaves. World Applied Sciences Journal 23(4), 500-506. http://dx.doi.org/10.5829/idosi.wasj.2013.23.04.368
Bari MN, Zubair M, Rizwan K, Rasool N, Bukhari IH, Akram S, Bokhari TH, Shahid M, Hameed M, Ahmad V. 2012. Biological activities of Opuntia monacantha cladodes. Journal of the Chemical Society of Pakistan 34(4), 990-995.
Berkowitz FE. 1995. Antibiotic resistance in bacteria. Southern Medical Journal 88(8), 797-780.
Brown MRW. 1975. Resistance of Pseudomonas aeruginosa. London, UK: John Wiley and Sons, 71.
Cruick-Shank R, Dugid JP, Marininon BP, Swain RHA. 1975. Screening of some Greek aromatic plants for antioxidant activity. Phytotherapy Research 17(2), 194-195.
Cushnie TPT, Lamb AJ. 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents 26, 343-356. http://dx.doi.org/10.1016/j.ijantimicag.2005.09.002
Dastagir G, Hussain F, Khan AA. 2012. Antibacterial activity of some selected plants of family Zygophyllaceae and Euphorbiaceae. Journal of Medicinal Plants Research 6(40), 5360-5368. http://dx.doi.org/10.5897/JMPR12.539
Gledhill D. 2008. The Names of Plants, 4th Ed. New York, USA: Cambridge University Press, 50, 312, 386.
Hindler FJ, Hochstein L, Howell A. 2007. Preparation of routine media and reagents used in antimicrobial susceptibility testing. In:Isenberg HD, Ed. Clinical Microbiology Procedures Handbook. Washington DC, USA: American Society for Microbiology (ASM) press 2(2), 5.14.1.1-5.14.1.4.
Holm L, Doll J, Holm E, Pancho J, Herberger J. 1997. World Weeds: Natural Histories and Distribution. New York, USA: John Wiley and Sons, Inc., 854-861.
Iqbal MJ, Hanif S, Mahmood Z, Anwar F, Jamil A. 2012. Antioxidant and antimicrobial activities of Chowlai (Amaranthus viridis L.) leaf and seed extracts. Journal of Medicinal Plants Research 6(27), 4450-4455. http://dx.doi.org/10.5897/JMPR12.822
Jain RA, Agarwal RC, Dubey D, Verma R, Jain R. 2012. Evaluation of antibacterial and antioxidant activity of fruits extract of Argemone mexicana Linn. International Journal of Pharmaceutical Innovations 2(1), 45-51.
Jorgensen JH, Turnidge JD. 2007. Susceptibility test methods: Dilution and disk diffusion methods. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, Eds. Manual of Clinical Microbiology, 9th Ed. Washington DC, USA: ASM Press, 1152-1172. http://dx.doi.org/10.1128/9781555817381.ch71
Khare CP. 2006. Indian Medicinal Plants, an Illustrated Dictionary. New Delhi, India: Springer-Verlag, 668.
Livermore DM. 2000. Antibiotic resistance in Staphylococci. International Journal of Antimicrobial Agents 16, 3-10. http://dx.doi.org/10.1016/S0924-8579(00)00299-5
Newman DJ, Cragg GM. 2007. Natural products as sources of new drugs over the last 25 years. Journal of Natural Product 70(3), 461-477. http://dx.doi.org/10.1021/np068054v
Priya, GS, Radhika R, Siddhuraju P. 2012. Antioxidant and antimicrobial activity of traditional Indian leafy vegetables: Mukia maderaspatana and Solanum trilobatum. International Journal of Pharmacy and Pharmaceutical Sciences 4(2), 513-521.
Raja W, Ovais M, Dubey A. 2013. Phytochemical screening and antibacterial activity of Lawsonia inermis leaf extract. International Journal of Microbiology Research 4(1), 33-36. http://dx.doi.org/10.5829/idosi.ijmr.2013.4.1.6679
Shibu BS, Devi BC, Moin S, Wesley S. 2013. Evaluation of bioactive potential of Coelogyne nervosa A. Rich. An endemic medicinal orchid of western Ghats, India. Asian Journal of Pharmaceutical and Clinical Research 6(1), 114-118.
Woldeyes S, Adane L, Tariku Y, Muleta D, Begashaw T. 2012. Evaluation of antibacterial activities of compounds isolated from Sida rhombifolia Linn. (Malvaceae). Natural Products Chemistry and Research 1, 101. http://dx.doi.org/10.4172/2329-6836.1000101
Annam Zikrea, Zaheer-ud-din Khan, Muhammad Waheed, 2016. Antibacterial potential of the crude extracts of Trianthema portulacastrum L.. Int. J. Biosci., 9(6), 19-27.
Copyright © 2016 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.