Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

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Research Paper 03/03/2024
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Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

Blé Yatanan Casimir, Toe Evelyne, Zouh Bi Zahouli Faustin, Dadié Adjehi
Int. J. Biosci.24( 3), 42-49, March 2024.
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Abstract

Aeromonas hydrophila is an opportunistic microorganism. It is widely involved in frog infections. Hoplobatrachus occipitalis is the species of frog most consumed in the different localities of Côte d’Ivoire. This research aimed to study the extracellular virulence factors and antibiotic resistance profiles of A. hydrophila strains isolated from H. occipitalis. The determination of extracellular virulence factors were performed onto 113 strains of A. hydrophila isolated from frogs H. occipitalis using conventional methods. The antimicrobial susceptibility tests were performed by the disk diffusion method using 20 isolates of A. hydrophila. All strains isolated produce nuclease (100%) but the ability to produce haemolysins, lipases, amylases and proteases varied from one isolate to another These microorganisms were the most resistant to Amoxicillin (95%), Ampicillin (90%), Tetracyclin (85%), Ceftriaxone (80%) Chloramphenicol (75%). All strains of A. hydrophila have developed multi-resistance to different antibiotics used. The multiple antibiotic resistance indices ranged from 0.41 to 0.83. These data show the degree of pathogenicity of the strains isolated from frogs. The consumption of this species could constitute a threat to consume them if hygiene measures are not taken into account.

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Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493

Ble YC, Yobouet BA, Dadié A. 2016. Consumption, proximate and mineral composition of edible frog Hoplobatrachus occipitalis from midwest areas of Côte d’Ivoire. African Journal of Science and Research 5(3), 16-20. https://www.researchgate.net/profile/Adjehi_Dadie/publication/315702287

Castro-Escarpulli G, Figueras MJ, Aguilera-Arreola GL, Soler RE, Fernandez, Aparicio GO, Guarro J, Chacon MR. 2003. Characterisation of Aeromonas spp. Isolated from frozen fish intended for human consumption in Mexico. International journal of Food Microbiology, 84: 41-49. https://doi.org/10.1016/S0168-1605(02)003.93-8

Elkamouny BM, Rasha ME, Younis GA. 2020. Prevalence and antibiotic resistance of Aeromonas hydrophila and Staphylococcus aureus isolated from seafood in Egypt. Mansoura Veterinary Medical Journal 4, 173-179. http://dx.doi.org/10.35943/mvmj.2020.47607.1009

Forbes MR, David L, McRuer, Rutherford L, Pamela. 2004. Prevalence of Aeromonas hydrophila in relation to timing and duration of breeding in three species of Ranid frog. Ecoscience 11, 282-285. https://www.jstor.org/stable/42901620

Hadeel A, Majeed A. 2011. Characterization of Aeromonas hydrophila isolated from patients with Diarrhea. Journal of Kerbala University 9, 33-42. https://iraqjournals.com/article_19061_0.html

Jalal A, Fatin N, Ashaari MM, John A, Yunus K, Saad S, Omar MN. 2010. Antibiotic resistance microbes in tropical mangrove sediments, East Coast Peninsular Malaysia. African Journal of Microbiology Research 4, 640-645. https://www.academia.edu/11749653

John N, Hatha AAM. 2013. Distribution, extracellular virulence factors and drug resistance of motile aeromonads in fresh water ornamental fishes and associated carriage water. International Journal of Aquaculture 17(3), 92-100. http://dx.doi.org/10.5376/ija.2013.03.0017

Koné M, Coulibaly KJ, Yao KE, Vakou SN, Yao KR, Dosso M, N’douba V. 2022. Aeromonas isolated from lagoon Tilapias in Côte D’Ivoire: diversity, distribution and potential virulence. American Journal of Microbiological Research 10(1), 34-39. https://pubs.sciepub.com/ajmr/10/1/5/index.html

Kouassi AE, Cisse M, Koussemon M, Ouattara A, Gourene G. 2017. Isolation and Identification of Aeromonas hydrophila, Pathogen of Farmed Tilapia (Oreochromis niloticus) in Region Agneby-Tiassa in Côte d’Ivoire. International Journal of Science and Research 7, 1914-1918. http://dx.doi.org/10.21275/ART20181068

Koudou AA, Ngazoa SK, Konan KF, Aka E, Addablah A, Coulibaly ND, Sina KSK, Atta DH, Guessend N, Ahoussi S, Dosso M. 2020. Occurrence of multidrug-resistant bacteria in aquaculture farms in Côte d’Ivoire (West Africa). African Journal of Microbiology Research 14(5), 182- 188. http://dx.doi.org/10.5897/AJMR2019.9277

McMahon MAS. 2000. The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres. Journal of Applied Microbiology 89, 415- 442. http://dx.doi.org/10.1046/j.1365-2672.2000.01129.x

Mohneke M, Onadeko AB, Hirschfeld M, Rödel MO. 2010. Dried or fried: amphibians in local and regional food markets in West Africa. Traffic Bulletin 45, 193- 202. TRAFFIC BULLETIN 22(3) March 2010 (PDF, 2.5 MB)

Nawaz M, Khan SA, Khan AA, Sung K, Tran Q, Kerdahi K, Steele R. 2010. Detection and characterization of virulence and integrons in Aeromonas veronii isolated from catfish. Food Microbiol 27, 327-331. https://doi.org/10.1016/j.fm.2009.11007

Olaniran AO, Nzimande SBT, Mkize NG. 2015. Antimicrobial resistance and virulence signatures of Listeria and Aeromonas species recovered from treated wastewater effluent and receiving surface water in Durban, South Africa. BioMed Central Microbiology 15, 15-235. http://dx.doi.org/10.1186/s12866-015-0570-x

Onadeko AB, Egonmwan RI, Saliu JK. 2011. Edible amphibian species: Local knowledge of their consumption in Southwest Nigeria and their nutritional value. West African Journal of Applied Ecology 19, 67-76. https://www.ajol.info/index.php/wajae/article/view/77567

Pemberton JM, Kidd SP, Schmidt R. 1997. Secreted enzymes of Aeromonas. FEMS Microbiology Letters, 152(1), 1-10. https://doi.org/10.1111/j.1574-6968.1997.tb10401.x

Rakici E, Altunisik A, Sahin K, Ozgumus OB. 2021. Determination and molecular analysis of antibiotic resistance in Gram-negative enteric bacteria isolated from Pelophylax sp. in the Eastern Black Sea Region. Akademiai Kiado. Acta Veterinaria Hungarica 69(3), 223-233. https://doi.org/10.1556/004.2021.00039

Saka BA, Adeyemo OK, Odeseye AO. 2017. Multiple antibiotic resistance indices of Aeromonas hydrophila isolates of muscle of catfish (Clarias gariepinus, burchell 1822) from selected markets in Ibadan, Nigeria. African Journal of Microbiology Research 14(5), 182-188. http://dx.doi.org/10.5897/AJMR2019.9277

Sarkar A, Saha M, Roy P. 2012. Identification and Typing of Aeromonas hydrophila through 16S rDNA-PCR Fingerprinting. Journal of Aquaculture Research and developpment 3(4), p. http://dx.doi.org/10.4172/2155-9546.1000146

Sarter S, Nguyen HNK, Hung LT, Lazard J, Montet D. 2007. Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish. Food Control 18, 1391-1396. https://doi.org/10.1016/j.foodcont.2006.10.003

Son R, Rusul G, Sahilah AM, Zainuri A, Raha AR, Salmah I. 1997. Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, telapia (Telapia mossambica). Letters in Applied Microbiology 24, 479-482. https://doi.org/10.1046/j.1472-765X.1997.00156.x

Stratev D, Vashin I, Rusev V. 2012. Prevalence and survival of Aeromonas spp. in foods-a review. Revue Médécine Vétérinaire 163, 486-494. https://www.academia.edu/22980388/Prevalence_and_survival_of_Aeromonas_spp_in_foods_a_review

Takahashi E, Ozaki, Fujii H, YoshioKobayashi H, Yamanaka H. 2014 “Properties of Hemolysin and Protease Produced by Aeromonas trota”, Plos One 9(3), 2014. http://dx.doi.org/10.1371/journal.pone.0091149

Thi HHP, Kim DH, Van CTQ, Nguyen PT, Nguyen TL. 2023. Prevalence and antibiotic resistance of Aeromonas schubertii causing internal white spot disease on snakehead fish, Channa striata, in the Mekong Delta, Vietnam. Journal of the World Aquaculture Society 54, 1260-1276. https://doi.org/10.1111/jwas.12954

Yadav S, Kumar VD, Kumar PP, Kumar DA, Sood N. 2014. Phenotypic and genotypic identification of Aeromonas species from aquatic environment. International Journal of Aquatic Science 5, 3-20. https://www.researchgate.net/publication/278764092