Antibiotic susceptibility and molecular detection of MEXT gene of Pseudomonas aeruginosa isolated from burned patients
Paper Details
Antibiotic susceptibility and molecular detection of MEXT gene of Pseudomonas aeruginosa isolated from burned patients
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic human pathogen and a leading cause of disease and death in burned patients. Nosocomial infections caused by P. aeruginosa are increasing worldwide that can be attributed to uncontrolled use of antibiotics in hospitals and community. The present study was aimed to decipher the antibiotic susceptibility and molecular detection of MEXT gene in multidrug resistant P. aeruginosa isolates from burned patients. A total of 100 swab samples from burned patients were collected and cultured on cetrimide agar plates followed by enrichment in nutrient broth. Bio characterization was done for positive pigment producing isolates. Antibiotic susceptibility was assessed by Kirby Bauer disk diffusion method to commonly used antibiotics; amikacin, tobramycin, ciprofloxacin, colistin, carbenicillin, meropenem, and ceftazidim. The MEXT gene was amplified in multidrug resistant (MDR) isolates. It was found that 44% isolates were positive for P. aeruginosa giving pigment production and positive citrate and oxidase tests. Antibiogram results revealed that 56.8% isolates (13/44) were multidrug resistant. The MEXT gene in selected MDR isolates was detected with 216bp size. In conclusion, colistin and meropenem could be effective in treating P. aeruginosa infections and MEXT gene modulates the induction of multidrug efflux system that further modulates antibiotic resistance to diverse range of antibiotics in P. aeruginosa.
CLSI C. 2012. M100-S25: Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Fifth Informational Supplement.
Derakhshan S, Peerayeh SN, Fallah F, Bakhshi B, Rahbar M. 2013. Identification of Extended Spectrum Beta-lactamase producing Klebsiella pneumoniae isolated from Intensive Care Unit ( ICU ) patients in three hospitals in Tehran. Infection, Epidemiology and Medicine 1, 9–13.
Doring G, Conway SP, Heijerman HG, Hodson ME, Hoiby N, Smyth A, Touw DJ. 2000. Antibiotic therapy against Pseudomonas aeruginosa in cystic fibrosis: a European consensus. European Respiratory Journal 16, 749-67.
Drenkard E. 2003. Antimicrobial resistance of Pseudomonas aeruginosa biofilms. Microbes and Infection 5, 1213-1219. https://doi.org/10.1016/j.micinf.2003.08.009
Fadeyibi IO, Raji MA, Ibrahim NA, Ugburo AO, Ademiluyi S. 2013. Bacteriology of infected burn wards of a teaching hospital in Southwest Nigeria 39, 168-173. https://doi.org/10.1016/j.burns.2012.02.005
Ghadiri H, Vaez H, Khosravi S, Soleymani E. 2012. The antibiotic resistance profiles of Bacterial strains isolated from patients with hospital-acquiredbloodstream and urinary tract infections.Critical CareResearch and Practice 2, 797-890. http://dx.doi.org/10.1155/2012/89.0797.
Hancock RE, Speert DP. 2000. Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and impact on treatment. Drug Resistance Updates 3, 247-255. https://doi.org/10.1054/drup.2000.0152
Hirsch EB, Tam VH. 2010. Detection and treatment options for Klebsiella pneumoniae carbapenemases (kpcs): an emerging cause of multidrug-resistant infection. Jornal of Antimicrobial Chemotherapy 65, 1119–25. https://doi.org/10.1093/jac/dkq108
Hope R, Warner M, Potz NC, Fagan EJ, James D, Livermore DM. 2006. Activity of tigecycline against ESBL-producing and AmpC-hyperproducing Enterobacteriaceae from South-East England. Journal of Antimicrobial Chemotherapy 58, 1312–4. https://doi.org/10.1093/jac/dkl414
Khan E, Ejaz M, Zafar A, Jabeen K, Shakoor S, Inayat R. 2010. Increased isolation of ESBL producing Klebsiella pneumoniae with emergence of carbapenem resistant isolates in Pakistan: Report from a tertiary care hospital. Journal of Pakistan MedicalAssociation 60,186–90.
Landman D, Bratu S, Kochar S, Panwar M, Trehan M, Doymaz M, Quale J. 2007. Evolution of antimicrobial resistance among Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae in Brooklyn, NY. Journal of Antimicrobial Chemotherapy 60, 78–82.
Lindsay D, VonHoly A. 2006.Bacterial biofilms within the clinical setting: What healthcare professionals should know. Journal of Hospital Infection 6, 1-28.
Livermore DM. 2002. Multiple Mechanisms of Antimicrobial Resistance in Pseudomonas aeruginosa: Our Worst Nightmare?. Clinical Infectious Diseases 34, 634-640.
Maseda H, Saito K, Nakajima A, Nakae T. 2000.Variation of the mexT gene, a regulator of the MexEF-OprN efflux pump expression in wild-type strains of Pseudomonas aeruginosa. FEMS Microbiology Letters 192, 107–12. https://doi.org/10.1111/j.1574-6968.2000.tb09367.x.
Mayhall CG. 2003. The Epidemiology of Burn Wound Infections: Then and Now. Clinical Infectious Diseases 37, 543-550. https://doi.org/10.1086/376993
Melaku S, Kibret M, Abera B. 2012. Antibiogram of nosocomial urinary tract infections in Felege Hiwot referral hospital.Ethiopia 12. http://dx.doi.org/10.4314/ahs.v12i29
Percival SL, Bowler PG, Russell D. 2005. Bacterial resistance to silver in wound care. Journal of Hospital Infection 60, 1-7.
Pessoa-Silva CL, Moreira BM, Almeida VC, Flannery B, Lins MCA, Sampaio JLM. 2003. Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit: risk factors for infection and colonization. Journal of Hospital Infection 53, 198–206.
Pirnay JP, Bilocq F, Pot B, Cornelis P, Zizi M, Van Eldere J, Deschaght P, Vaneechoutte M, Jennes S, Pitt T, De Vos D. 2009. Pseudomonas aeruginosa population structure revisited. PloS one 4, e7740.
Pruitt BA, Lindberg RB, McManus WF, Mason AD. 1983. Current approach to prevention and treatment of Pseudomonas aeruginosa infections in burned patients. Infectious Diseases 5, 889-897.
Richard P, Floch RL, Chamoux C, Pannier M, Espaze E, Richert H. 1994. Pseudomonas aeuroginosa Outbreak in a Burn Unit: Role of Antimicrobials in Emergence of Multiply Resistant Strains. Infectious Diseases 170, 377-383.
Schweizer HP. 2003. Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions. Genetics and Molecular Research 2, 48–62. https://doi.org/10.3923/pjbs.2007.924.927.
Stover CK, Pham XM, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Lory S, Olson MV. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01,an opportunistic pathogen. Nature 406, 959-964. https://doi.org/10.1038/35023079.
Waszczuk K, Gula G, Swiatkowski M, Olszewski J, Herwich W, Drulis-Kawa Z, Gutowicz J, Gotszalk T. 2012. Evaluation of Pseudomonas aeruginosa biofilm formation using piezoelectric tuning fork mass sensors. Sensors and Actuators B: Chemical 170, 7-12.
Whiteley M, Bangera MG, Bumgarner RE, Parsek MR, Teitzel GM, Lory S, Greenberg EP. 2001. Gene expression in Pseudomonas aeruginosa biofilms. Nature 413, 860-864.
Sidra Anam, Faisal Rasheed Anjum, Tayyaba Younas, Sajjad ur Rahman, Muhammad Usman (2018), Antibiotic susceptibility and molecular detection of MEXT gene of Pseudomonas aeruginosa isolated from burned patients; IJB, V13, N3, September, P276-282
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