Anticarcinogenic and antimicrobial assessment of copper oxide nanoparticles derived from the leaf extract of Catharanthus roseus

Paper Details

Research Paper 18/02/2024
Views (176) Download (15)

Anticarcinogenic and antimicrobial assessment of copper oxide nanoparticles derived from the leaf extract of Catharanthus roseus

Selvanayakam Sumitha, Esakiappan Subramanian, Kalaiyar Swarnalatha
J. Bio. Env. Sci.24( 2), 135-144, February 2024.
Certificate: JBES 2024 [Generate Certificate]


This study focuses on the biological examination of copper oxide nanoparticles (CuO NPs) synthesized using Catharanthus roseus leaf extract, which served both as reducing and stabilizing agents. The formation of these biogenic CuO NPs was analyzed through UV-visible, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The UV-visible spectral analysis revealed a peak at 273 nm, while FTIR provided insight into the functional groups associated with the NPs. Antibacterial testing showed inhibition zones (ZOI) and Minimum Inhibitory Concentration (MIC) against eight pathogenic bacterial strains, both positive and negative. Antifungal testing exhibited ZOI against four pathogenic fungal organisms. Furthermore, the nanoparticles displayed antioxidant potential, with an IC50 value of 58.55 µg/ml, indicating the scavenging ability. The in-vitro assessment of anticancer activity was carried out against the HT29 cell line, revealing an IC50 value of 37.99 µg/ml.


Al-Jassani MJ, Raheem HQ. 2017. Anti-bacterial activity of CuO nanoparticles against some pathogenic bacteria. Int. J. Chem. Tech. Res 10(2), 818-822.

Alahdal FA, Qashqoosh MT, Manea YK, Mohammed RK, Naqvi S. 2023. Green synthesis and characterization of copper nanoparticles using Phragmanthera austroarabica extract and their biological/environmental applications. Sustainable Materials and Technologies 35, e00540.

Amin F, Khattak B, Alotaibi A, Qasim M, Ahmad I, Ullah R, Ahmad R. 2021. Green synthesis of copper oxide nanoparticles using Aerva javanica leaf extract and their characterization and investigation of in vitro antimicrobial potential and cytotoxic activities. Evidence-Based Complementary and Alternative Medicine 2021, 5589703.

Bhavyasree PG, Xavier TS. 2022. Green synthesised copper and copper oxide based nanomaterials using plant extracts and their application in antimicrobial activity. Current Research in Green and Sustainable Chemistry 5, 100249.

Bajwa N, Mahal S, Singh PA, Jyoti K, Dewangan P, Madan J, Baldi A. 2023. Drug-polymer conjugates: Challenges, opportunities, and future prospects in clinical trials. Polymer-Drug Conjugates, 389-469p.

Bauer AW, Roberts Jr CE, Kirby WM. 1959. Single disc versus multiple disc and plate dilution techniques for antibiotic sensitivity testing.  Antibiotics Annual 7, 574-580.

Begum SN, Esakkiraja A, Asan SM, Muthumari M, Raj GV. 2019. Green Synthesis of Copper Oxide Nanoparticles Using Catharanthus roseus Leaf Extract and Their Antibacterial Activity. Int. J. Sci. Res. in Multidisciplinary Studies 5, 8.

Chakraborty N, Banerjee J, Chakraborty P, Banerjee A, Chanda S, Ray K,   Sarkar J. 2022. Green synthesis of copper/copper oxide nanoparticles and their applications: a review. Green Chemistry Letters and Reviews 15(1), 187-215.

Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved Standard M27-A3.

Dadi R, Azouani R, Traore M, Mielcarek C, Kanaev A. 2019. Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram-negative strains. Materials Science and Engineering: C 104, 109968.

Darroudi M, Ahmad MB, Abdullah AH, Ibrahim NA. 2011. Green synthesis and characterization of gelatin-based and sugar-reduced silver nanoparticles. International Journal of Nanomedicine, 569-574p.

Gahlawat G, Choudhury AR. 2019. A review on the biosynthesis of metal and metal salt nanoparticles by microbes. RSC advances 9(23), 12944-12967.

Rajbhoj A. 2011. Copper Oxide Nanoparticles: Synthesis, Characterization, and Their Antibacterial Activity, Journal of Cluster Science, 22, 121-129.

Jabarkhil M, Azizi AS, Imam SZ, Alrabbat A, Hasan KD, Hasan MH. 2023. Revolutionising Cancer Diagnosis and Treatment: A Review on Advancements in Nanomaterial-based Theranostics. International Journal of Engineering Materials and Manufacture 8(4), 106-123.

Manimaran K, Yuli Yanto DH, Kamaraj C, Selvaraj K, Pandiaraj S, Elgorban AM, Vignesh S, Kim H. 2023. Eco-friendly approaches of mycosynthesized copper oxide nanoparticles (CuONPs) using Pleurotus citrinopileatus mushroom extracts and their biological applications. Environmental Research 232, 116319.

Magaldi D, Silvia W, Camero T. 1997. Suceptibilidad de Candida albicans “In vitro” mediante los posos de diffusion.  Bol. venez. Infectol  5-8p.

Manzocco L, Anese M, Nicoli MC. 1998. Antioxidant properties of tea extracts as affected by processing. LWT-Food Science and Technology 31, 694-698.

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55-63.

Marshall NJ, Goodwin CJ, Holt SJ. 1995. A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth regulation 5, 69-84.

Rajagopal G, Nivetha A, Sundar M, Panneerselvam T, Murugesan S,  Parasuraman P. Kunjiappan S. 2021. Mixed phytochemicals mediated synthesis of copper nanoparticles for anticancer and larvicidal applications. Heliyon 7(6).

Siddiquee MA, Parray MD, Kamli MR, Malik MA, Mehdi SH, Imtiyaz K, Rizvi MM. Rajor HK, Patel R. 2021. Biogenic synthesis, in-vitro cytotoxicity, esterase activity and interaction studies of copper oxide nanoparticles with lysozyme. Journal of Materials Research and Technology 13, 2066-2077.

Saleh HM, Hassan AI. 2023. Synthesis and characterization of nanomaterials for application in cost-effective electrochemical devices. Sustainability 15(14), 10891.

Sajid M, Płotka-Wasylka J. 2020. Nanoparticles: Synthesis, characteristics, and applications in analytical and other sciences. Microchemical Journal 154, 104623.

Sardar R, Ahmed S, Shah AA, Yasin NA. 2022. Selenium nanoparticles reduced cadmium uptake, regulated nutritional homeostasis and antioxidative system in Coriandrum sativum grown in cadmium toxic conditions. Chemosphere 287, 132332.

Subramanian E, Subbulekshmi NL. 2016. Copper Oxide Incorporated Zeolite Catalyst Developed from Waste Coal Fly Ash Material and Its Catalytic Wet Peroxide Oxidative Degradation of Crystal Violet Dye. Journal of Advanced Chemical Sciences 1, p. 204–207

Sathish Kumar K, Raghavendra R, Mohamed Khalith SB, Mohammed Junaid Hussain D, Darul Raiyaan GI, Kantha Deivi A. 2021. Characterization, antibacterial and photocatalytic evaluation of green synthesized copper oxide nanoparticles. Biocatalysis and Agricultural Biotechnology 31, 101904.

Turakhia B, Divakara MB, Santosh MS, Shah S. 2020. Green synthesis of copper oxide nanoparticles: A promising approach in the development of antibacterial textiles. Journal of Coatings Technology and Research 17, 531-540.

Thambidurai S, Gowthaman P, Venkatachalam M, Suresh S. 2020. Enhanced bactericidal performance of nickel oxide-zinc oxide nanocomposites synthesized by facile chemical co-precipitation method. Journal of Alloys and Compounds 830, 154642.

Zahrah A. 2022. Green synthesis of copper oxide nanoparticles CuO NPs from Eucalyptus globoulus leaf extract: Adsorption and design of experiments. Arabian Journal of Chemistry 15, 103739.