Antidiabetic activity of in-vivo and in-vitro plants of Cleome gynandra L. in streptozotocin-induced-diabetic rats
Paper Details
Antidiabetic activity of in-vivo and in-vitro plants of Cleome gynandra L. in streptozotocin-induced-diabetic rats
Abstract
The aim of the present study is to evaluate the therapeutical potential of in-vivo and in-vitro plants of Cleome gynandra L. in alleviating diabetes by assessment of body weight and blood glucose level of STZ-induced diabetic rats. Ethanolic shoot extracts of C. gynandra used in the traditional management of diabetes. Nicotinamide 120mg/kg was used as a positive control. A glucometer was used for blood glucose level measurement and body weight was measured by weighing balance at intervals. Total cholesterol and triglycerides of the lipid profile were analyzed. Treatment of STZ-induced Wistar diabetic with the ethanolic plant extracts of C. gynandra (500mg/kg) compared to the glibenclamide standard drug, the in-vitro plant extracts of C. gyanandra were best reacted against body weight reduction and when compared to in-vivo shoots caused significant (P<0.001) reductions in the Blood Glucose Level (BGL). Administration of in-vitro shoot extracts showed a higher reduction in TC and TG levels than in-vivo shoot extracts TC and TG and control rats TC and TG. The drug consumption of diabetic-induced rats’ body weight and blood glucose level shows successful treatment. The ethanolic shoot extracts of in-vivo and in-vitro plants C. gynandra demonstrate anti-hyperglycemic activity, thereby confirming anti-diabetic potential and validating traditional medicine.
Alema NM, Periasamy G, Sibhat GG, Tekulu GH, Hibenmg. 2020. Antidiabetic Activity of Extracts of Terminalia brownii Fresen. Stem Bark in Mice. J. Exp. Pharmacol 20(12), 61-71.
Ali W, Shaikh H, Abdullah A, Khanam S. 2016. Standardization of unani antidiabetic tablet- Qurse Tabasheer. Pharmcog. Res 8(2), 147-152.
Ardalani H, Hejazi Amiri F, Hadipanah A, Kenneth TK. 2021. Potential antidiabetic phytochemicals in plant roots: A review of in-vivo studies. J. Diabetes Metab. Disord. 20, 1837-1854.
Arika WM, Abdirahman YA, Mawia MA, Wambua KF, Nyamai DM. 2015. In-vivo Antidiabetic Activity of the Aqueous Leaf Extract of Croton macrostachyus in Alloxan Induced Diabetic Mice. Pharm. Anal. Acta. 6, 447.
Artiss JD, Zak B. 1997. Measurement of Cholesterol Concentration. In: Rifai N, Warnick, G.R., Dominiczak, MH, Eds. Handbook of lipoprotein testing. Washington: AACC Press 99-114.
Bassey S, Antia Jude E, Okokon Emem E, Umoh John Udobang A. 2010. Antidiabetic activity of ethanolic leaf extract of Panicum maximum. Int. J. Drug. Dev. and Res. 2(3), 488-492.
Berhan BY, Awgichew SY. 2021. Medicinal Plant Extracts Evaluated In-vitro and In-vivo for Antidiabetic Activities in Ethiopia: Bases for Future Clinical Trials and Related Investigations. Evidence-Based Complementary and Alternative Medicine, Article ID 9108499, 1-24.
Calpe-Berdiel L, Escolà-Gil JC, Ribas V, Navarro-Sastre A, Garcés- Garcés J, Blanco-Vaca F. 2005. Changes in intestinal and liver global gene expression in response to a phytosterol-enriched diet. Atherosclerosis 181, 75-85.
Chanda S. 2014. Importance of pharmacognostic study of medicinal plants: An overview. J. Pharm. Phytochem 2(5), 69-73.
Cho SY, Park JY, Park EM. 2002. Alteration of hepatic antioxidant enzyme activities and lipid profile in streptozotocin induced diabetic rats by supplementation of dandelion water extract. Clinica Chemica Acta. 317, 109-117.
Day C, Bailey CJ. 1998. Hypoglycemic agents from traditional plant treatments for diabetes. Int. Ind. Biotech. 50, 5-8.
Dongdong Z, Karuppusamy A, Yuehu W, Yu Z, Jun Y, Pyae PH, Aye MM, Jianwen L, Angkhana I, Xuefei Y. 2021. Evaluation on Antidiabetic Properties of Medicinal Plants from Myanmar. The Scientific World Journal Article ID 1424675, 1-10.
Gautam RK, Sharma S, Sharma K, Goyal S. 2020. Evaluation of comparative Anti-arthritic Activity of Tranditionally well documented medicinal plants in rats. Indian Journal of Pharmaceutical Sciences 85(5), 781-786.
Gray AM, Flatt PR. 1997. Nature’s own pharmacy: The diabetes perspective. Proc. Nutr. Soc. 56, 507- 517.
Huang PL, Huang P, Huang H, Lee-Huang SI. 1992. Developing from traditional medicinal plants. Chem Ind. 8, 290-93.
Lin Y, Sun Z. 2010. Current views on type 2 Diabetes. J. Endocrinology 204(1), 1-11.
Mahmood A, Qureshi RA. 2012. Antimicrobial activities of three species of family mimosaceae. Pak. J. Pharm. Sci. 25, 203-206.
Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G. 1998. Experimental NIDDM: Development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 47, 224-229.
Mishra SS, Moharana, SK Dash MR. 2011. A review on Cleome gynandra. Int. J. Res. Pharm. and Chem. 1(3), 681-689.
Mohtasheem UHM, Salman A, Munnawar S, Iqbal A. 2011. Analgesic and Antiemetic activity of Cleome viscosa L. Pak. J. Bot. 43(1), 119-122.
Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. 2006. Leads from Indian medicinal plants with hypoglycemic potentials, J. Ethnopharm 106(1), 1-28.
Murugan P, Pari L. 2006. Antioxidant effect of tetrahydrocurcumin in streptozotocin-nicotinamide induced diabetic rats. Life Sci. 79, 1720-1728.
Musila W, Kisangau D, Muema J. 2002. Conservation Status and Use of Medicinal Plants by Traditional Medical Practitioners in Machakos District, Kenya. National Museums of Kenya.
Orech FO, Akenga T, Ochora J, Friis H, Aagaard-Hansen J. 2005. Potential toxicity of some traditional leafy vegetables consumed in Nyango’ma Division, Western Kenya. Afr J Food & Nut Sci 5, 1-14.
Partha Pradip Adhikari, Satya Bhusan Paul. 2018. Medicinally important plant Cleome gynandra: A phytochemical and pharmacological explanation. Asian J. Pharm. Clin. Res. 11(1), 21-29.
Rahman S, Jan G, Jan FG, Rahim HU. 2021. Phytochemical Screening and Antidiabetic, Antihyperlipidemic, and Antioxidant Effects of Leptopus Cordifolius Decne. In Diabetic Mice. Front. Pharmacolo 12, 1-12.
Rajkumar L, Govindarajulu P. 1991. Increased degradation of dermal collagen in diabetic rats. Ind. J. Exp. Bio. 29, 1081-1083.
Ramachandran S, Rajasekaran A, Manisenthilkumar K. 2012. Investigation of hypoglycemic, hypolipidemic and antioxidant activities of aqueous extract of Terminalia paniculata bark in diabetic rats. Asian Pacific Journal of Tropical Biomedicine 2(4), 262-268.
Reher G, Slijepcevic M, Krans L. 1991. Hypoglycaemic activity of triterpenes and tannins from Sarcopoterium spinosum and two Sanguisorba species. Planta Medica 57, A57-A58.
Seedevi P, Ganesan A, Moovendhan M, Mohan K, Sivasankar P, Loganathan S, Vairamani S, Shanmugam A. 2020. Anti-diabetic activity of crude polysaccharide and rhamnoseenriched polysaccharide from G. lithophila on Streptozotocin (STZ)-induced in Wistar rats. Scientific Reports. Nature research 10, 556.
Sudan I, Karthikeyan AVP. 2019. Indirect organogenesis of Cleome gynandra L. using leaf explants an important medicinal plant. Int. J. Pharm. Sci. and Res. 10(9), 4287-92.
Swanston-Flatt SK, Flatt PR, Day C, Bailey CJ. 1991. Traditional dietary adjuncts for the treatment of Diabetes mellitus. Proc. Nutr. Soc. 50, 641-650.
Tomoda M, Shimada K, Konno C, Hikino H. Structure of Panaxan B. 1985. A hypoglycaemic glycan of Panax ginseng roots. Phytochemistry 24, 2431-2433.
Trinder P. 1969. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J. Clin. Pathol. 22(2), 158-161.
World Health Organization. 2015. Diabetes Fact. Sheet No. 312.
Yallow RS, Black H, Villazan M, Berson SA. 1960. Comparison of plasma insulin levels following administration of tolbutamide and glucose. Diabetes 9, 356-362.
I. Sudan, AVP. Karthikeyan (2022), Antidiabetic activity of in-vivo and in-vitro plants of Cleome gynandra L. in streptozotocin-induced-diabetic rats; JBES, V21, N6, December, P94-100
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