Antifungal activity of the clove essential oil from Syzygium aromaticum on Paecilomyces variotii agent of pistachio dieback

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Short Communications 01/06/2014
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Antifungal activity of the clove essential oil from Syzygium aromaticum on Paecilomyces variotii agent of pistachio dieback

Laleh Yazdanpanah, Neda Mohamadi
J. Bio. Env. Sci.4( 6), 42-45, June 2014.
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Abstract

Pistachio dieback is a serious disease in Rafsanjan (Iran) farms caused by Paecilomyces variotii. The aim of the present study was to investigate fungicidal effects of essential oil of cloves on Paecilomyces variotii agent of pistachio dieback. In order to evaluate the antifungal properties of Syzygium aromaticum, essential oil from leaf and bud of clove were obtained from hydrodistillation. The isolates were cultured in sabouraud dextrose agar for 48 h at 25°C. The concentration of 100, 200 and 400 ppm of the oil was used. Every day a diameter of fungal growth on culture medium was measured using a calibrated microscope. The lowest fungal growth was observed at concentrations of 400 ppm oil (1.421 Cm). Maximum fungal growth was observed in the control sample (6.1 Cm). Based on results of this study, it is recommended that the concentration of 400 ppm clove oil is used to reduce the growth rate of the Paecilomyces.

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Arina B, Iqbal A. 2002. In vitro fungitoxicity of the essential oil of Syzygium aromaticum. World Journal of Microbiology and Biotechnology 18(4), 317-319. http://dx.doi.org/10.1023/a: 1015242901360

Briozzo J. 1989. Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. Journal of Applied Microbiology 66, 69-75. http://dx.doi.org/0.1111/j.1365-2672.1989.tb02456.x

Cai L, Wu CD. 1996. Compounds from Syzygium aromaticumprocessing growth inhibitory activity against oral pathogens. Journal of natural products 59, 987-990. http://dx.doi.org/10.1021/np960451q

Chaieb K, Hajlaoui H, Zmantar T, Nakbi KAB, Rouabhia M, Mahdouani K, Bakhrouf A. 2007a. The chemical composition and biological activity of essential oil, Eugenia cryophyllata (Syzygium aromaticum L. Myrtaceae): a short review. Phytotherapy Research, 21(6), 501-506. http://dx.doi.org/ 10.1002/ptr.2124

Chaieb K, Zmantar T, Ksouri R, Hajlaoui H, Mahdouani K, Abdelly C and Bakhrouf A. 2007b. Antioxidant properties of essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. Mycosis 50(5), 403-406. http://dx.doi.org/10.1111/j.1439-0507.2007.01391.x

Chami F, Chami N, Bennis S, Trouillas J, Remmal A. 2004. Evaluation of carvacrol and eugenol as prophylaxis and treatment of vaginal candidiasis in an immunosuppressed rat model. Journal of Antimicrobial Chemotherapy 54(5), 909-914. http://dx.doi.org/10.1093/jac/dkh436

Devi KP, Nisha SA, Sakthivel R, Pandian SK. 2010. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. Journal of Ethnopharmacology 130(1), 107-115. http://dx.doi.org/10.1016/j.jep.2010.04.025

Francisco Cortés-Rojas D, Regina Fernandes de Souza C, Pereira Oliveira W. 2014. Clove (Syzygium aromaticum): a precious spice. Asian Pacific Journal of Tropical Biomedicine 4(2), 90-96.

Giordani R, Regli P, Kaloustian J, Mikail C, Abou L, Portugal H. 2004. Antifungal effects of various oils against Candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris. Phytotherapy Research 18(12), 990-995. http://dx.doi.org/10.1002/ptr.1594

Houbraken J, Varga J, Rico-Munoz E, Johnson S, and Samson R. 2008. “Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii). Microbiology 74, 16131619.

Lee KG, Shibamoto T .2001. Antioxidant property of aroma extract isolated from clove buds [Syzygium aromaticum  (L.)  Merr.  Et  Perry].Food  Chemistery 74, 443-448. http://dx.doi.org/10.1016/S0308-8146(01)00161-3

Park MJ, Gwak KS, Yang I, Choi WS, Jo HJ, Chang WJ, Jeung EB and Hoi IG. 2007. Antifungal activities of the essential oils in Syzygium aromaticum (L.) Merr. Et Perry and Leptospermum betersonni Bailey and their constituents against various dermatiphytes. Journal of Microbiology 45(5), 460-465.

Pawar VC, Thaker VS. 2006. In vitro efficacy of 75 essential oil against Aspergillus niger. Mycosis 49(4), 316-323. http://dx.doi.org/10.1111/j.1439-0507.2006.01241.x

Rana IS, Rana AS, Rajak RC. 2011. Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Brazilian Journal of Microbiology 42(4), 1269-1277. http://dx.doi.org/10.1590/S1517-83822011000400004.

Shan B, Cai YZ, Sun M, Corke H. 2005. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. Journal Agric Food Chemistry 53(20), 7749-7759.

Sofia PK, Prasad R, Vijay VK, Srivastava AK. 2007. Evaluation of antibacterial activity of Indian spices against common foodborne pathogens. International Journal of Food Sciences and Nutrition 42(8), 910-915.

Yang YC, Lee SH, Lee WJ, Choi DH and Ahn YJ. 2003. Ovicidal and adulticidal effects of Eugenia cryophyllata bud and leaf oil compounds on Pediculus capitis. Journal of Agricultural and Food Chemistry 51(17), 4884-4888. http://dx.doi.org/10.1021/jf034225f