Antifungal activity of the clove essential oil from Syzygium aromaticum on Paecilomyces variotii agent of pistachio dieback
Paper Details
Antifungal activity of the clove essential oil from Syzygium aromaticum on Paecilomyces variotii agent of pistachio dieback
Abstract
Pistachio dieback is a serious disease in Rafsanjan (Iran) farms caused by Paecilomyces variotii. The aim of the present study was to investigate fungicidal effects of essential oil of cloves on Paecilomyces variotii agent of pistachio dieback. In order to evaluate the antifungal properties of Syzygium aromaticum, essential oil from leaf and bud of clove were obtained from hydrodistillation. The isolates were cultured in sabouraud dextrose agar for 48 h at 25°C. The concentration of 100, 200 and 400 ppm of the oil was used. Every day a diameter of fungal growth on culture medium was measured using a calibrated microscope. The lowest fungal growth was observed at concentrations of 400 ppm oil (1.421 Cm). Maximum fungal growth was observed in the control sample (6.1 Cm). Based on results of this study, it is recommended that the concentration of 400 ppm clove oil is used to reduce the growth rate of the Paecilomyces.
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