Int. J. Biosci.13( 5), 309-316, November 2018
Tomato is economically important horticultural crop worldwide, especially in Pakistan. Rhizoctonia solani is a serious threat for tomato growing areas, responsible for considerable crop and yield losses. This study was designed to minimize these huge economic losses and to regain the attention of formers which was shifted towards other crops as compare to this profitable crop. Leaves extract of three medicinal plants, cinnamon (Cinnamomum verum), moringa (Moringa oleifera) and clove (Syzygium aromaticum) were investigated against R. solani causing damping off of tomato. Three different concentrations (1%, 2% and 3%) were used to check the efficacy of plant extracts. Three different trials were conducted as invtro under CRD, greenhouse and field under RCBD. Antifungal potency test showed that the maximum growth inhibition was observed at 3% concentration followed by 2 and 1% of each plant extracts. In vitro clove leave extract shown highest antifungal activity which causes complete mycelial inhibition 3% concentration. At 3% concentration efficacy of plants extracts was also examined on disease incidence as well as plant growth development in greenhouse and field experiment. Clove leave extract at 3% concentration recorded highly significant in disease reduction and other plant growth parameters. Thus, clove leaves are best choice for managing R. solani associated with damping off disease of tomato.
Achimu P, Scholsser A. 1992. Effect of neem seed extracts (Azadirachta indica A. Juss) against downy mildew (Plasmopara viticola) of grape vine. Medicine Faculty Landbouw. University Ghet 57(2), 423-431.
Agrios GN. 2005. Plant Pathology, Elsevier Academic Press California, USA., p 922.
Chana MY, Pathan MA, Solangi GR, Wondiar M. 1997. Studies on Rhizoctonia solani (Kuhn) causing root rot of lentil. Sarhad Journal of Agriculture 11, 495-499.
Coa L, Qiu Z, You J, Tan H, Zhou S. 2004. Isolation and characterization of endophytic Streptomyces from surface-sterilized tomato (Lycopersicon esculentum) roots. Letters in Appliel Microbiology 39, 425-30.
Ejechi BO, Nwafor OE, Okoko FJ. 1999. Growth inhibition of tomato-rot fungi by phenolic acid and essential oil extracts of pepper fruit Dennetia tripetala. Food Research International 2, 395-399.
Food and Agriculture Organization. 2010. FAOSTAT. 31 December 2012. http://faostat.fao.orgaccessed
Gary C, Tchamitchian M. 2011. Modeling and management of fruit production: the case of tomatoes. Food process modeling. CRC press, Boca and Raton; 201-229.
Gopalachari NC. 1984. Tobacco. Indian council of Agricultural Research, New Dehli, India, p 327.
Huang X, Zhang N, Yong X, Yang X, Shen Q. 2011. Biocontrol of Rhizoctonia solani damping off disease in cucumber with Bacillus pumilus SQR-N43. Microbiology Research 167, 135–43.
Islam MT, Faruq AN. 2012. Effect of some medicinal plant extracts on Damping-off disease of winter vegetable. World Applied Science Journal, 17(11), 1498-1503.
Kumar A, Shukla R, Singh P, Prasad CS, Dubey NK. 2008. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post-harvest fungi infestation of food commodities. Innovative Food Science and Emerging Technologies, 9, 575-580.
Lucas GB. 1975. Diseases of Tobacco (3rd edition), Biological consulting associates, Raleigh, N.C., p 621.
Lucas GB, Campbell CL, Lucas LT. 1997. Introduction to plant disease identification and management. CBS Pub. and Distributors, New Delhi., 364.
Mckinney HH. 1923. Biological control of nematode pests by natural enemies. Annual Review Phytopathology 18, 415-440.
Moussa TAA. 2002. Studies on biological control of sugar beet pathogen Rhizoctonia solani Kühn. Online Journal of Biology Science 2, 800-4.
Neha D, Dawande AY. 2010. Biocontrol of soil borne plant pathogen Rhiozoctonia solani using Trichoderma spp. and Pseudomonas fluorescens. Asiatic Journal of Biotechnology Research 1, 39-44.
Parmeter JR, Sherwood RT, Platt WD. 1969. Anastomosis grouping among isolates of Thanatephorus cucumeris. Phytopathology 59, 1270-8.
Sabuquillo P, Cal AD, Melgarejo P. 2006. Biocontrol of tomato wilt by Penicillium oxalicum formulations in different crop conditions. Biological Control, 37(3), 256-265.
Sadeghi A, Hessan AR, Askari H, Aghighi S, Shahidi BGH. 2006. Biological control potential of two Streptomyces isolates on Rhizoctonia solani, the causal agent of damping-off of sugar beet. Pakistan Journal of Biological Sciences 9, 904-10.
Seema M, Sreenivas SS, Rekha ND, Devaki NS. 2011. In vitro studies of some plant extracts against Rhizoctonia solani Kuhn infecting FCV tobacco in Karnataka Light Soil, Karnataka, India. Journal of Agricultural Technology 7(5), 1321-1329.
Sneh BLB, Akira O. 1991. Identification of Rhizoctonia species. The American Phytopathological Society, St. Paul, Minnesota, USA. ISBN 089054123 X. 129.
Steel RDD, Torrie JH, Dicky D. 1997. Principles and Procedures of Statistics: A Biometrical Approach. (3rd Ed) Mc. Graw Hill Book Co. Inc. New York, USA.
Ushiki J, Hayakawa Y, Tadano TB. 1996. Medicinal plants for suppressing soil borne plant diseases. 1. Screening for medicinal plants with antimicrobial activity in roots. Soil Science Plant Nutrient 42, 423-426.