Antimicrobial activity of Foeniculum vulgare Mill essential oil against antibiotic resistance human pathogen

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Research Paper 01/07/2014
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Antimicrobial activity of Foeniculum vulgare Mill essential oil against antibiotic resistance human pathogen

Fereshteh Javadian, Mohammad Ali Mashhadi, Aliakbar Nasiri, Zahra Sepehri, Zohre Kiani, Zahra Shahi
J. Bio. Env. Sci.5( 1), 285-290, July 2014.
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The aim study antimicrobial activity of Foeniculum vulgare Mill essential oil against antibiotic resistance human pathogen. All 36 strains of (12 Klebsiella pneumoniae, 12 E. coli and 12 Staphylococcus aureus) isolated from urine culture of hospitalized patients during the years 2011- 2012. In this study, the essential oil of Foeniculum vulgare Mill obtained by hydrodistillation and the minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this essential oil. However, overall, E. coli were resistance to 8 of the agent including ceftazidime (50%), tetracyclin (75%), erythromycin (58.3%), cefixime (41.6%) , penicillin (83.3%), ampicillin (58.3%), nalidixic acid (58.3%). Antibiotic susceptibility of K. pneumoniae isolates was evaluated for 4 antimicrobial. However k. pneumoniae were resistance to 4 of the agent including ceftazidime (33.3%) ,cefixime (58.3%), erythromycin (75%), tetracyclin (50%) and S. aureus were resistance to 6 antimicrobial , cefixime (33.3%), trimethoprim-sulfamethoxazol (41.66%), penicillin (50%), oxacillin (83.3%), ceftazidime (66.6%) and vancomycin (8.3%).The highest MIC values of essential oil were found to be 250ppm against four E. coli and three K. pneumoniae and two of MIC value for S.aureus was 10ppm.


Branther A, Grein E. 1994. Antibacterial activity of plant extracts used externally in traditional medicine. Journal of Ethnopharmacology 44, 35-40.

Iwv MM, Ezeuywu CD, Okungi CO, Sanson DR, Tempestu MS. 1993. Antibacterial activity and terpenoids of the essential oils of Hyptis sauveolensis. International journal of crude drug research 28, 73-76.

Piccaglia R, Marotti M. 2001. Characterization of Some Italian Types of Wild Fennel (Foeniculum vulgare Mill.) Journal of Agricultural and Food Chemistry 49, 239-244.

Ozbek H, Ugras S, Dulger H, Bayram I, TuncerI, Ozturk G. 2003. Hepatoprotective effect of Foeniculum vulgare essential oil. Fitoterapia 74, 317-319.

Gori L, Gallo E, Mascherini V, Mugelli A, Vannacci A and Firenzuoli F. 2012. Review Article: Can Estragole in Fennel Seed Decoctions Really Be Considered a Danger for Human Health? A Fennel Safety Update Evidence-Based Complementary and Alternative Medicine.

Chaouche T, Haddouchi F, Lazouni HA, Bekkara FA. 2011. Phytochemical study of the plant plant Foeniculum vulgare Mill. Der pharmacia letter 3(2), 329-33.

Forbes  BA,  Sahm  DF,  Weissfeld  AS.  2007. Bailey  &  Scott`s  diagnostic  microbiology.  12th  ed. Missouri: Mosby Co. 323-333.

Mohsenzadeh M. 2007. Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium Pakistan Journal of Biological Sciences 10, 3693–3697.

Cantore PL, Iacobelli NS, Marco AD, Capasso F, Senatore F. 2004. Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller Var. vulgare (Miller). Essential oils. Journal of Agricultural and Food Chemistry 52, 7862–7866.

Dadalioglu I, Evrendilek GA. 2004. Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens. Journal of Agricultural and Food Chemistry 52, 8255–8260.

Kaur GJ, Arora DS. 2008. In-vitro antibacterial activity of three plants belonging to the family Umbelliferae. International Journal of Antimicrobial Agents 31, 393–395. 10.1016/j.ijantimicag.2007.11.007

Rojas A, Hernandez L, Pereda-Miranda R, and Mata R. 1992. Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants, Journal of Ethnopharmocology 35, 275-283.

Saviu C, Marina I, Grumezescu AM, Bleotu C, Chifiriuc C, Mihaiescu D, Lazar V. Phytochemical composition of the fennel fruits essential oil and its influence on prokaryotic cells growth and pathogenic features. Bioninterface research in applied chemistry 2(2), 300-5.

Akond MA, Alam S, Hassan MR, Shirin M. 2009. Antibiotic Resistance of Escherichia coli Isolated From Poultry and Poultry Environment of Bangladesh. Internet Journal of food safety 11, 19-23.

Tricia DM, McLaughlin W, Brown PD. 2006. Antimicrobial resistance of Escherichia coli isolates from broiler chickens and humans. BMC Veterinary Research 2, 7.

Zamani A, Yousefi Mashouf R, Ebrahimzadeh NamvarA, Alikhani MY. 2013. Detection of magA Gene in Klebsiella spp. Isolated from Clinical Samples. Iranian Journal of Basic Medical Sciences 16, 173-76.

Sikarwar AS and Batra HV. 2011. Prevalence of Antimicrobial Drug Resistance of Klebsiella pneumoniae in India. International Journal of Bioscience, Biochemistry and Bioinformatics 1(3).

Zarifian A, Sadeghian A, Sadeghian H, Ghazvini K, Safdari H. 2012. Antibiotic Resistance Pattern of Hospital Isolates of Staphylococcus aureus in Mashhad-Iran During 2009 – 2011 Archives of Clinical Infectious Diseases 7(3), 96-98.