Antioxidant activity and total polyphenol and flavonoid contents of Cissus doeringii Gilg & M. Brandt (Vitaceae) and Diospyros mespiliformis Hochst. ex DC. (Ebenaceae), used for treating malaria

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Research Paper 07/09/2024
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Antioxidant activity and total polyphenol and flavonoid contents of Cissus doeringii Gilg & M. Brandt (Vitaceae) and Diospyros mespiliformis Hochst. ex DC. (Ebenaceae), used for treating malaria

Sylla Youssouf , Diomandé Awa, Kouamé Amoin Gervaise, Koffi Akessé Georges, Azokou Alain, Koné Mamidou Witabouna
Int. J. Biosci.25( 3), 137-147, September 2024.
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Abstract

Cissus doeringii (Vitaceae) and Diospyros mespiliformis (Ebenaceae) are two plants traditionally used to treat malaria that generates strong oxidative stress. This study was conducted to evaluate the in vitro antioxidant activity and the total polyphenol and flavonoid contents of the aqueous and methanolic extracts of leaves of these two plants. The antioxidant activity of extracts was evaluated by the free radical scavenging methods (DPPH and ABTS). Polyphenol and flavonoid contents of the extracts were determined by the spectrocolorimetric method. The results of the antioxidant activity revealed that the DPPH radical inhibition percentages of the extracts ranged from 34.95 ± 3.4 to 70.84% with IC50 ranging from 5.52 to 36.59 μg/mL and those of the ABTS radical from 52.98 ± 6,69 to 81.32 ± 3.08% with IC50 between 5.98 and 34.12 μg/mL. The methanolic extract of the leaves of Cissus doeringii showed the best antiradical activity (ABTS) with an IC50 of 5.98 μg/mL, in comparison to those of the references, ascorbic acid (5.30 μg/ml) and trolox (5.73 μg/ml). The antioxidant capacities of the extracts ranged from 59.79 ± 7.55 to 68.26 ± 5.76 mg of TEg-1 dry matter. The polyphenol contents were between 336.36 ± 0.58 and 466.67 ± 0.58 mg EAG/g of extract and the total flavonoid contents between 202.14± 0.26 and 254.06 ± 0.26 mg ECA/g extract. These two plants could be sources of natural antioxidants able to prevent the oxidative stress associated with Plasmodium infection and other diseases.

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