Antioxidant activity of essential oil from Lawsonia inermis Linn from Pakistan

Paper Details

Research Paper 01/03/2018
Views (278) Download (7)

Antioxidant activity of essential oil from Lawsonia inermis Linn from Pakistan

Zafar Iqbal, M. Khalid Saeed, Khuram Shehzad, Shaista Nawaz
Int. J. Biosci.12( 3), 110-115, March 2018.
Certificate: IJB 2018 [Generate Certificate]


The present study was designed to assess the constituents of essential oil of lawsonia innermis Linn by GC-MS and measure the antioxidant ability by using DPPH stable radical. Essential oil was extracted from the leaves of lawsonia Inermis Linn by hydro-distillation using modified Dean Stark Apparatus, followed by drying with anhydrous sodium sulphate. GC-MS studies were prformed for constituents determination by comparison the retention indices and mass spectra with those obtained the library on National Institute of Standard and Technology (NIST). Antioxidant activity of the essential oil of the lawsonia immerimis was performed by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Light yellow colored oil with 0.4% yield was obtained. The chemical constituents were analyzed by GC-MS, which revealed 21 compounds, out of which 13 were identified and eucalyptol (18.141%). α-pinene (13.345%) and linalool (10.527%) were major components. The essential oil was tested for DPPH scavenging assay and it was found concentration dependant and maximum antioxidant activity 88% was observed for sample at 100 μl concentration while BHT showed activity 92% for the similar concentration. The present study suggested that essential oil of lawsonia innermis is a good source of natural antioxidant.


Abdulmoneim MA. 2007. Evaluation of Lawsonia Inermis Linn, (Sudanese Henna) Leaf extracts as antimicrobial agent, Research J. Biol Sci 2(4), 419.

Adebola O, Oyedeji A, Olusegun E, Wilfried A. 2011. Koenig, Essential Oil Composition of Lawsonia inermis L. Leaves from Nigeria, Published online. 28 Nov.

Andreadis AA, Hazen SL, Comhair SA, Erzurum SC. 2003. Oxidative and nitrosative events in asthma. Free Radic Biol Med 35, 213.

Anjum P, Mohammad Q. 2005. pollen flora of pakistan–xliii. lythraceae, Pak. J.Bot 37(1), 1.

Asami S, Manabe H, Miyake J, Tsurudome Y, Hirano T. 1997. Cigarette smoking induces an increase in oxidative DNA damage,8-hydroxydeoxyguanosine in a central site of the human lung. Carci-nogenesis 18, 1763.

Asmah R, Susi E, Patimah I, Taufiq Y, Yun H, Mohammad F. 2006. Chemical Constituents, Antioxidant activity and Cytotoxic Effects of Essential Oil from Strobilanthes crispus and Lawsonia inermis. J. Biological Sci 6(6), 1005.

Dhalla NS, Temsah RM, Netticadan T. 2000. Role of oxidative stress in cardiovascular diseases. J Hypertens 18, 655.

Haddad K, Mohammad H, Dezashibi Z. 2007. Phenolic Compounds and Antioxidant Activity of Henna Leaves Extracts (Lawsonia Inermis), World J. Dairy & Food Sci 2(1), 38.

Halliwell B, Gutteridge JMC. 1999. Free Radicals in Biology and Medicine 3rd Ed. New York: Oxford University Press.

Jenner P. Oxidative stress in Parkinson’s disease. Ann Neurol 53, S26 (2003).

Kasparova S, Brezova V, Valko M, Horecky J, Mlynarik V. 2005. Study of the oxidative stress in a rat model of chronic brain hypo-perfusion. Neurochem Int 46, 601.

Kerr S, Brosnan MJ, McIntyre M, Reid JL, Dominiczak AF, Hamilton CA. 1999. Superoxide anion production is increased in a model of genetic hypertension: role of the endothelium. Hypertension 33, 1353.

Krinoky NI. 1992. Mechanism of action of biological antioxidant. Proceeding of the Society for Experimental Biology and Medicine 210, 248.

Kukreja RC, Hess ML. 1992. The oxygen free-radical system: from equations through membrane–protein interactions to cardiovascular injury and protection. Cardiovasc Res 26, 641.

Lugas A, Hovari J, Sagi KV, Biro L. 2003. The role of antioxidant phytonutrients in the prevention of disease. Acta Biologica Szegediensis 47(1-4), 119.

Lyras L, Cairns NJ, Jenner A, Jenner P, Halliwell B. 1997. An assessment of oxidative damage to proteins, lipids, and DNA in brain from patients with Alzheimer’s disease. J Neurochem 68, 2061.

Marnett LJ. 1999. Lipid peroxidation and DNA damage by malondialdehyde. Mutat Res 424, 83.

Oyedeji O, Oziegbe M, Taiwo FO. 2011. Antibacterial, antifungal and phytochemical analysis of crude extracts from the leaves of Ludwigia abyssinica A. Rich. and Ludwigia decurrens Walter, J Medicinal Plants Res 5, 1192.

Rice-Evan, CA., Dipalock AT. 1993. Current status of antioxidant therapy. Free Radical Biological and Med 15, 77.

Sayre LM, Smith MA, Perry G. 2001. Chemistry and biochemistry of oxidative stress in neurodegenerative disease. Curr Med Chem 8, 721.

Siems WG, Grune T, Esterbauer H. 1995. 4-Hydroxynonenal formation during ischemia and reperfusion of rat small-intestine. Life Sci 57, 785.

Stadtman ER. 2004. Role of oxidant species in aging. Curr Med Chem. 11, 1105.

Toshniwal PK, Zarling EJ. 1992. Evidence for increased lipid peroxidation in multiple sclerosis. Neurochem Res.17, 205.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160, 1.

Wang MY, Dhingra K, Hittelman WN, Liehr JG, deAndrade M, Li DH. 1996. Lipid peroxidation-induced putative malondialdehyde–DNA adducts in human breast tissues. Cancer Epidemiol Biomarkers Prev 5, 705.

Zarrin F, rizvi1, Rabia M, Fayyaz C, Muhammad Z. 2013. Antibacterial and antifungal activities of Lawsonia inermis, Lantana camara and swertia angustifolia, pak. J. Bot 45(1), 275.