Antioxidant activity of methanol extracts of Enhalus acoroides and Thalassia hemprichii from the coastal water of Carmen, Agusan Del Norte, Philippines
Paper Details
Antioxidant activity of methanol extracts of Enhalus acoroides and Thalassia hemprichii from the coastal water of Carmen, Agusan Del Norte, Philippines
Abstract
Antioxidants play an important role in apoptosis, gene expression, and ion transportation and may reduce the risk of many diseases by protecting the cells against the effect of free radicals. The antioxidant potential of methanolic extracts of the seagrasses Thalassia hemprichii and Enhalus acoroides collected from the coastal water of Carmen, Agusan Del Norte, Philippines was determined using Aluminum chloride complex forming assay for total flavonoid content, Folin–Ciocalteu reagents with analytical grade gallic acid as the standard for the total phenolic content, DPPH, ABTS and FRAP. The results showed that methanol extract of Thalassia hemprichii had the highest content of total phenolics and flavonoids which values were 2.651 and 2.734 mgGA/g respectively. The strongest free radical scavenging activity (DPPH) of the extracts was recorded by seagrass Enhalus acoroides which value was 0.301 mgtrolox/g. While the methanol extracts of Thalassia hemprichii recorded the maximum radical cation decolorization power (ABTS) and Ferric ion reducing antioxidant power (FRAP) which values were 0.252 and 1.119 mgtrolox/g respectively. The antioxidant activity determined by DPPH, ABTS and FRAP demonstrated a strong linear relationship with the phenolics and flavonoids. The results suggested that the sea grasses Thalassia hemprichii and Enhalus acoroides have strong antioxidant potential and could be a source of natural antioxidant compounds.
Athi Perumalsami T, Rajeswari VD, Poorna SH. 2010. Antioxidant activity of seagrasses and seaweeds. Botany 53(3), 251–7.
Baehaki A, Supriadi A, Pratama MC. 2016 Antioxidant Activity of Methanol Extract of Halodule uninervis Seagrass from the Coastal of Lampung, Indonesia. Research Journal of Pharmaceutical, Biological, and Chemical Sciences, 173-177 p.
Benzie IF, Strain JJ. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a measure of “Antioxidant Power”: The FRAP assay. Journal Analytical Biochemistry 239(1), 70-6.
Chaillou LL, Nazareno MA. 2006. New method to antioxidant activity of polyphenols. Journal of Agriculture and Food Chemistry 54(22), 8397-402.
Camposs AM, Lissi EA. 1996. Kinetics of the reaction between 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) derived radical cations and phenols. International Journal of Chemical Kinetics 29(3), 219-24.
Cannac M, Ferrat L, Barboni T. 2007. The influence of tissue handling on the flavonoid content of the aquatic plant Posidonia oceanica. Journal Chemical Ecology 33(5), 1083-8.
Dumay O, Costa J, Desjobert JM, Pergent G. 2004. Variations in the concentrations of phenolic compounds in the seagrass Posidonia oceanica under conditions of competition. Phytochemistry 65, 3211-3220.
Fortes MD. 2013. A Review: Biodiversity Distribution and Conservation of Philippine Seagrasses. Philippine Journal of Science 142, 95-111, Special Issue.
Gringnon-Dubois M, Rezzonico B, Alcoverro T. 2012. Regional scale patterns in seagrass defenses: Phenolic acid content in Zosteranoltii. Estuarine, Coastal and Shelf Science 114, 18-22.
Gavin NM, Durako MJ. 2011. Localization and antioxidant capacity of flavonoids from intertidal and subtidal Halophila johnsonii and Halophila decipiens. Aquatic Botany 95, 242-247.
Green EP, Short FT. 2003. World Atlas of Seagrasses. Berkeley, USA: University of California Press.
Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry47, 3954-3962.
Kannan RR, Arumugam R, Anantharaman P. 2010.In vitro antioxidant activities of ethanol extract from Enhalus acoroides (L.F.) Royle. Asian Pacific Journal of Tropical Medicines 3(11), 898-901.
Kannan RR, Arumugam R, Thangaradjou T, Anantharaman P. 2013. Phytochemical constituents, antioxidant properties and p-coumaric acid analysis in some seagrasses. Food Research International 54(1), 1229-36.
Kannan RR, Arumugam R, Meenakshi S, Anantharaman P. 1984. Thin layer chromatography analysis of antioxidant constituents from seagrasses of Gulf of Mannar Biosphere Reserve, South India. International Journal Chemistry and Technology. Seagrasses. Aquatic Botany 20, 351-7.
Kuo J, Den Hartog C. 2001. Seagrass taxonomy and identification key. In: SHORT, F.T AND COLES, R.G., eds. Global Seagrass Research Methods. Amsterdam, Elsevier 31-58.
Larson RA. 1999. Plant defenses against oxidative stress. Archives Insect Biochemistry and Physiology 29(2), 175-86.
Libin B, Sankar TV, Chandramohanakumar N. 2017. Changes in phenolic compounds in sea grass against change in the ecosystem. Phytojournal.Com/archives/2017/6(3), PartL./6-3-62-162.pdf.
Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26(2), 211-9.
Pulido R, Bravo L, Saura-Calixto F. 2000. Antioxidant Activity of Dietary Polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agriculture and Food Chemistry 48(8), 3396-402.
Ragupathi Raja Kannan R, Rajasegaran A, Thirunavukarasu T. 2013. Phytochemical constituents, antioxidant properties and p-coumaric acid analysis in some seagrasses. Food Research International.
Rice-Evants C, Miller NJ, Bolwell GP, Bramley PM, Pridham JB. 1995. The relative antioxidants activities of plant-derived polyphenolic flavonoids. Free Radical Research 22, 375-383.
McMillian C. 1984. The condensed tannins (proanthocyanidins) in seagrasses. Aquatic Botany 20, 351-357.
Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26(2), 211-9.
Neelima CSS, Seenivasan R. 2015. DPPH radical scavenging activity of selected Seagrasses from South East Coast of India. International Journal of Advanced Research 3, 950 – 956.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicines 26(9), 1231-7.
Santoso J, Anwariyah S, Rumiantin RO, Putri AP, Ukhty N, Yoshie-Stark Y. 2012. Phenol Content, Antioxidant Activity and Fibers profile of four tropical seagrasses from Indonesia. Journal of Coast Development Research 15(2), 189-96.
Somanah MJ, Abdoulrahman N, Bhagooli R. 2012. Assessment of phenol content and antioxidant activities of shallow-water macroalgae from Mauritius. University of Mauritius Research Journal, 18A, 28-53.
Sullivan ML. 1994. The taxonomy of “seagrasses” surveyed from the higher taxa down through to the family level [Online]. Florida International University. Available from: http://www2.fiu.edu/~seagrass/class/bot5647/maureen.htm.
Szabo MR, Iditoiu C, Chambre D, Lupea AX. 2007. Improved DPPH Determination for Antioxidant Activity Spectrophotometric Assay. Chemistry Papers 61(3), 214-6.
Villano D, Fernandez-Pachon MS, Moya ML, Troncoso AM, Garcia- Parilla MC. 2007. Radical scavenging ability of phenolic compounds towards DPPH free radical. Talanta 71, 230–235.
Zapata O, McMillan C. 1979.Phenolic acids in seagrasses. Aquatic Botany 307-17.
Efren Tangon, Oliva P. Canencia, Romeo M. Del Rosario, Elvinia R. Alivio, 2020. Antioxidant activity of methanol extracts of Enhalus acoroides and Thalassia hemprichii from the coastal water of Carmen, Agusan Del Norte, Philippines. Int. J. Biosci., 16(6), 178-184.
Copyright © 2020 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.