Antioxidant activity of methanol extracts of Enhalus acoroides and Thalassia hemprichii from the coastal water of Carmen, Agusan Del Norte, Philippines

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Research Paper 01/06/2020
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Antioxidant activity of methanol extracts of Enhalus acoroides and Thalassia hemprichii from the coastal water of Carmen, Agusan Del Norte, Philippines

Efren Tangon, Oliva P. Canencia, Romeo M. Del Rosario, Elvinia R. Alivio
Int. J. Biosci.16( 6), 178-184, June 2020.
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Abstract

Antioxidants play an important role in apoptosis, gene expression, and ion transportation and may reduce the risk of many diseases by protecting the cells against the effect of free radicals. The antioxidant potential of methanolic extracts of the seagrasses Thalassia hemprichii and Enhalus acoroides collected from the coastal water of Carmen, Agusan Del Norte, Philippines was determined using Aluminum chloride complex forming assay for total flavonoid content, Folin–Ciocalteu reagents with analytical grade gallic acid as the standard for the total phenolic content, DPPH, ABTS and FRAP. The results showed that methanol extract of Thalassia hemprichii had the highest content of total phenolics and flavonoids which values were 2.651 and 2.734 mgGA/g respectively. The strongest free radical scavenging activity (DPPH) of the extracts was recorded by seagrass Enhalus acoroides which value was 0.301 mgtrolox/g. While the methanol extracts of Thalassia hemprichii recorded the maximum radical cation decolorization power (ABTS) and Ferric ion reducing antioxidant power (FRAP) which values were 0.252 and 1.119 mgtrolox/g respectively. The antioxidant activity determined by DPPH, ABTS and FRAP demonstrated a strong linear relationship with the phenolics and flavonoids. The results suggested that the sea grasses Thalassia hemprichii and Enhalus acoroides have strong antioxidant potential and could be a source of natural antioxidant compounds.

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