Antioxidant and anti-inflammatory activity of Pleurotus citrinopileatus Singer and Pleurotus sajor-caju (Fr.) Singer

Paper Details

Research Paper 14/08/2025
Views (689)
current_issue_feature_image
publication_file

Antioxidant and anti-inflammatory activity of Pleurotus citrinopileatus Singer and Pleurotus sajor-caju (Fr.) Singer

P. Maheswari, P. Madhanraj, V. Ambikapathy, P. Prakash, A. Panneerselvam
J. Biodiv. & Environ. Sci. 27(2), 90-96, August 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

The antioxidant and anti-inflammatory properties of cultured mycelia from two species of the oyster mushroom, specifically Pleurotus species including Pleurotus citrinopileatus and P. sajor caju were investigated. The mushroom extracts were evaluated for their capacity to scavenge DPPH, hydrogen peroxide and free radicals. The antioxidant and anti-inflammatory activity was assessed through various assays, specifically the reducing power assay, hydrogen peroxide scavenging (H2O2) assay, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which exhibited similar activities across different concentrations of 100, 200, 300, 400 and 500 µg/ml. These diverse antioxidant activities of edible mushrooms compared to standard ascorbic acid were evaluated using a spectrophotometric method. The maximum percentage of antioxidant activities was observed in P. citrinopileatus and P. sajor caju at a concentration of 500 µl in DPPH assay when compared with low concentration as well as other methods in P. citrinopileatus mushroom and Vitamin C as standard. The effect of anti-inflammatory properties of aqueous extract of P. citrinopileatus with higher concentration of 500µl was good results when compared with methanolic extract and P. sajor caju mushroom with diclofenac sodium as standard. Nevertheless, the efficacy of both edible mushrooms suggested their promising potential for antioxidant properties in vitro condition. The antioxidant and anti-inflammatory activity exhibited by edible mushrooms holds considerable significance, as this activity substantially enhances their nutraceutical characteristics, thereby increasing their overall nutritional values.

Aida FMNA, Shuhaimi M, Yazid M, Maaruf AG. 2009. Mushroom as a potential source of prebiotics: A review. Trends in Food Science & Technology 20, 567-575.

Alona T, Remigiusz O, Joanna H. 2025. Physicochemical, functional and antioxidative characteristics of oyster mushrooms. Applied Sciences 15, 1655.

Alves M, Ferreira IFR, Dias J, Teixeira V, Martins A, Pintado M. 2012. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Medica 78, 1707–1718.

Banukie WJANJ, Shiroma MH, Chandanie AW, Gita HF, Thusitha DUA, Sugandhika TS. 2020. Anti-inflammatory activity of Pleurotus ostreatus, a culinary medicinal mushroom, in Wistar rats. Evidence-Based Complementary and Alternative Medicine 2020, 6845383.

Block G, Patterson B, Subar A. 1992. Fruits, vegetables and cancer prevention: A review of the epidemiological evidence. Nutrition and Cancer 18, 1–29.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2, S178–S180.

Chang ST, Miles PG. 2008. Mushrooms: Cultivation, nutritional value, medicinal effect, and environmental impact. 2nd edition. CRC Press, Boca Raton, Florida, USA.

Colak A, Ozlem F, Sesli E. 2009. Nutritional composition of some wild edible mushrooms. Turkish Journal of Biochemistry 34, 25–31.

Dundar A, Okumus V, Ozdemir S, Yildiz A. 2013. Antioxidant properties of cultured mycelia from four Pleurotus species produced in submerged medium. International Journal of Food Properties 16, 1105–1116.

Elsayed EA, El Enshasy H, Wadaan MAM, Aziz R. 2014. Mushrooms: A potential natural source of anti-inflammatory compounds for medical applications. Mediators of Inflammation 2014, 805841.

Ergönül PG, Akata I, Kalyoncu F, Ergönül B. 2004. Fatty acid compositions of six wild edible mushroom species. The Scientific World Journal 4, Article ID not specified.

Galappaththi MCA, Dauner L, Madawala S, Karunarathna SC. 2021. Nutritional and medicinal benefits of oyster (Pleurotus) mushrooms: A review. Fungal Biotechnology 1, 65–87.

Ghafoor A, Naizi AR. 2024. Pleurotus spp: An ultimate solution to the emerging calamities of the world. New Zealand Journal of Botany, 1–38.

Gillman MW, Cupples AL, Gagnon D, Posner BM, Ellison RC, Wolf PA, Castelli WP. 1995. Protective effect of fruits and vegetables on development of stroke in men. JAMA – Journal of the American Medical Association 273, 1113–1117.

Guillamón E, García-Lafuente A, Lozano M. 2010. Edible mushrooms: Role in the prevention of cardiovascular diseases. Fitoterapia 81, 715–723.

He JZ, Ru QM, Dong DD, Sun PL. 2012. Chemical characteristics and antioxidant properties of crude water-soluble polysaccharides from four common edible mushrooms. Molecules 17, 4373–4387.

Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR. 2010. Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chemistry 119, 1443–1450.

Hyeok JC, Jeong WC, So JP, Sag HL, Jin HH, Youngki P, Kyoung TL, Jin BJ. 2025. Anti-inflammatory mechanisms of Pleurotus citrinopileatus: Inhibition of MAPK and NF-κB signaling pathways, and activation of ROS/PI3K/Nrf2/HO-1 signaling pathway in LPS-stimulated RAW264.7 cells. Journal of Microbiology and Biotechnology 35, e2501012.

Jan S, Petr M, Jan T, Matej B. 2022. Antioxidant and anti-inflammatory activity of five medicinal mushrooms of the genus Pleurotus. Antioxidants 11, 1569.

Jayasuriya WJABN, Shiroma MH, Chandanie AW, Gita HF, Thusitha DUA, Sugandhika TS. 2020. Anti-inflammatory activity of Pleurotus ostreatus, a culinary medicinal mushroom, in Wistar rats. Evidence-Based Complementary and Alternative Medicine 2020, 6845383.

Jedinak A, Dudhgaonkar S, Wu Q. 2011. Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling. Nutrition Journal 10, 52.

Kalač P. 2013. A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. Journal of the Science of Food and Agriculture 93, 209–218.

Mattila P, Könkö K, Eurola M. 2001. Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. Journal of Agricultural and Food Chemistry 49, 2343–2348.

Musieba F, Okoth S, Mibey RK, Wanjiku S, Moraa K. 2012. Suitability of locally available substrates for cultivation of the Kenyan indigenous golden oyster mushroom (Pleurotus citrinopileatus Singer). American Journal of Food Technology 7, 650–655.

Mycek MJ, Harvvey RA, Champe PC. 2001. Anti-inflammatory drugs and autacoids. In: Harvvey R, Champe PC (eds.), Lippincott’s illustrated reviews: Pharmacology. Lippincott Williams and Wilkins, Pennsylvania, USA, 401.

Nor-Aleesya MN, Wan-Mohtar WAAQI, Inshirah I, Aida FMNA, Raseetha S. 2023. Stability of pigments from Termitomyces sp. (termite mushroom), Pleurotus citrinopileatus (yellow oyster mushroom) and Pleurotus djamor (pink oyster mushroom) as natural food colouring. Food Research 7, 84–92.

Oyaizu M. 1986. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 44, 307–315.

Patel S, Goyal A. 2012. Developments in mushrooms as anticancer therapeutics: A review. 3 Biotech 2, 1–15.

Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10, 1003–1008.

Selima K, Aminul I, Ugur C, Perihan G, Narayan CC. 2011. Nutritional qualities and antioxidant activity of three edible oyster mushrooms (Pleurotus spp.). NJAS – Wageningen Journal of Life Sciences 72–73, 1–5.

Smith HA. 2014. Production of antimicrobials and antioxidants from filamentous fungi. PhD Thesis, National University of Ireland.

Wang S, Marcone MF. 2011. The biochemistry and biological properties of the world’s most expensive underground edible mushroom: Truffles. Food Research International 44, 2567–2581.

Related Articles

The effects of climate change on built structures in Benin-city, Edo state, Nigeria

A. E. Ilenre, Y. H. Tashok, E. N. Uhumuavbi, J. Biodiv. & Environ. Sci. 27(3), 39-49, September 2025.

A review of the ecological impacts of water pollution on lakes: From diatom assemblages to migratory bird population declines

Arunpandiyan Murugesan, Roshy Ann Mathews, S. Rajakumar, Prashanthi Devi Marimuthu, J. Biodiv. & Environ. Sci. 27(3), 23-38, September 2025.

Agroforestry in woody-encroached Sub-Saharan savannas: Transforming ecological challenges into sustainable opportunities

Yao Anicet Gervais Kouamé, Pabo Quévin Oula, Kouamé Fulgence Koffi, Ollo Sib, Adama Bakayoko, Karidia Traoré, J. Biodiv. & Environ. Sci. 27(3), 10-22, September 2025.

Extreme rainfall variability and trends in the district of Ouedeme, municipality of Glazoue (Benin)

Koumassi Dègla Hervé, J. Biodiv. & Environ. Sci. 27(3), 1-9, September 2025.

Heterosis breeding, general and specific combining ability and stability studies in pearl millet: Current trends

Ram Avtar, Krishan Pal, Kavita Rani, Rohit Kumar Tiwari, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 117-124, August 2025.

Combining ability, heterosis and stability for yield and fibre quality traits in cotton: Breeding approaches and future prospects

Rohit Kumar Tiwari, Krishan Pal, R. P. Saharan, Ram Avtar, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 109-116, August 2025.

Bridging the COPD awareness gap in marginalized populations: Findings from a multicentre study in Khalilabad, Sant Kabir Nagar, Uttar Pradesh, India

Anupam Pati Tripathi, Jigyasa Pandey, Sakshi Singh, Smita Pathak, Dinesh Chaudhary, Alfiya Mashii, Farheen Fatima, J. Biodiv. & Environ. Sci. 27(2), 97-108, August 2025.

Mangrove abundance, diversity, and productivity in effluent-rich estuarine portion of Butuanon River, Mandaue City, Cebu

John Michael B. Genterolizo, Miguelito A. Ruelan, Laarlyn N. Abalos, Kathleen Kay M. Buendia, J. Biodiv. & Environ. Sci. 27(2), 77-89, August 2025.