Antiradical activity and polyphenol content of ethanolic extracts of Propolis

Paper Details

Research Paper 01/04/2012
Views (306) Download (10)

Antiradical activity and polyphenol content of ethanolic extracts of Propolis

Njintang Yanou Nicolas, Tatsadjieu Ngoune Leopold, Ngakou Albert, Danra Dourandi, Tchuenguem-Fohouo Fernand Nestor
Int. J. Biosci.2( 4), 56-63, April 2012.
Certificate: IJB 2012 [Generate Certificate]


Propolis is a natural substance produced by honeybees from resinous products collected from plants. Its ethanolic extract is currently commercialised in Cameroon under the brand name Promax C. This study investigated the total polyphenols, tannins and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of Promax C (6 samples) compared to the freshly ethanol extracted propolis (15 samples). The results revealed that all Promax C samples tested showed evidence of radical scavenging properties with values ranging from 28 to 70 %. Although the ethanolic samples had lower phenol contents (8.6 – 17.0 g/100 g), their anti-oxidant activities (38.8 – 85.9 %) were systematically higher than those of Promax C. In addition the Promax C manufactured in 2006 systematically exhibited the highest scavenging activity (67.3%) and polyphenol contents (772.8 mg/L) compared to those manufactured in 2004 (mean scavenging activity 43.7 %; mean polyphenol contents, 227.8 mg/L). While there was a linear relationship between the radical scavenging activity and the polyphenols or tannin content in the Promax samples, this was not the case with the fresh ethanolic extract. The Cameroonians propolis exhibited higher scavenging activity which could justify their commercialisation and role in the management of some chronic diseases. However, the activity of Promax tends to decrease with aging, and this needs to be investigated.


Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T. 2007. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry 101, 1383–1392

Al-Mamary M, Al-Meeri A, Al-Habori M. 2002. Antioxidant activities and total phenolics of different types of honey. Nutrition Research 22, 1041–1047.

Bankova VS, Castro SL, Marcucci MC. 2000. Propolis: recent advances in chemistry and plant origin. Apidologie 31, 3–15.

Banskota AH, Nagaoka T, Sumioka LY, Tezuka Y, Awale S, Midorikawa K, Matsushige K, Kadota S. 2002. Antiproliferative activity of the Netherlands propolis and its active principles in cancer cells lines. Journal of Ethnopharmacology 80, 67–73.

Castaldo S, Capasso F. 2002. Propolis, an old remedy used in modern medicine. Fitoterapia 73, S1– S6.

Cerutti P. 1994. Oxy-radicals and cancer. Lancet 344, 862–863.

Chen Y-W, Wu S-W, Ho K-K, Lin S-B, Huang C-Y, Chen C-N. 2008. Characterization of Taiwanese propolis collected from different locations and seasons. Journal of the Science of Food and Agriculture 88, 412–419.

Dean R, Gieseg T, Davies MJ. 1993. Reactive species and their accumulation on radical damaged proteins. Trends in Biochemical Sciences 18, 437–441.

Gutteridge JMC, Halliwell B. 1994. Free radicals and antioxidants in aging and disease: fact or fantasy. In Antioxidants in nutrition, health, and disease pp. 111–135. Oxford, UK: Oxford University Press.

Kähkönen MP, Hopia AI, Vuorela HJ, Rauha, J-P, Pihlaja K, Kujala TS. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry 47, 3954–3962.

Kumazawa S, Taniguchi M, Suzuki Y, Shimura M, Kwon M-S, Nakayama T. 2002. Antioxidant activity of polyphenols in carob pods. Journal of Agricultural and Food Chemistry 50, 373–377.

Mohammadzadeh S, Sharriatpanahi M, Hamedi M, Amanzadeh Y, Ebrahimi SES, Ostad SN. 2007. Antioxidant power of Iranian propolis extract. Food Chemistry 103, 729–733.

Murad JM, Calvi SA, Soares AMVC, Bankova V, Sforcin JM. 2002. Effects of propolis from Brazil and Bulgaria on fungicidal activity of macrophages against Paracoccidioides brasiliensis. Journal of Ethnopharmacology 79, 331–334.

Nieva Moreno MI, Isla MI, Sampieto AR, Vattuone MA. 2000. Comparison of the free radical- scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology 71, 109–114.

Okada Y, Okada M. 1998. Scavenging effect of water soluble proteins in broad beans on free radicals and active oxygen species. Journal of Agricultural and Food Chemistry 46, 401–406.

Ozen S, Akyol O, Iraz M, Sogut S, Ozugurlu F, Ozyurt H. 2004. Role of caffeic acid phenethyl ester, an active component of propolis, against cisplatin- induced nephrotoxicity in rats. Journal of Applied Toxicology 24, 27–35.

Sforcin JM, Fernandes JrA, Lopes CAM, Bankova V, Funari SRC. 2000. Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology 73, 243–249.