Antispermatogenic effects of seed extract of Caesalpinia bonducella in Swiss mice
Paper Details
Antispermatogenic effects of seed extract of Caesalpinia bonducella in Swiss mice
Abstract
Ceasalpinia bonducella, a shrub profusely growing in tropical countries of the world, is known for its medicinal efficacy. However, the male antifertility effects of the plant are not at all documented. Hence, the present study is designed to investigate the male antifertility effects of the seed extract taking Swiss mice as the experimental model. The crude ethanolic seed extract of Caesalpinia bonducella was fed to group of sexually matured Swiss mice at a dose of 500 mg/Kg of body weight at every alternate day in such a way that every mice received 10 doses. The corresponding controls were given distilled water at the same dose and time. The experimental group of mice depicted a significant decrease (p≤0.05) in testis weight compared to controls. Sperm count in experimental groups of mice declined significantly (p≤0.0001) compared to controls. But, there was no increase in abnormal sperm count profile, testicular oxidative stress or body weight indicating that, the seed extract does not have any adverse effect in the testicular physiology. Testicular histology of the treated groups indicated intact seminiferous tubules, but, without spermatids. Flow cytometric analysis of the testis indicated cell arrest at G1 with concomitant decline in DNA synthesis at S phase and sign of tumor development. The finding of the study demonstrates that Caesalpinia bonducella seed extract can be used as an oral male contraceptive after specific isolation and modulation of the drug.
Abramoff P, Thomson RC. 1966. An Experimental approach of Biology. Freeman and Company, London.
Arif T, Mandal TK., Kumar N, Bhosale JD, Hole A, Sharma GL. 2009. In vitro and in vivo antimicrobial activities of seeds of Caesalpinia bonduc (Linn.) Roxb. Journal of Ethnopharmacology123, 177-180. https://doi.org/10.1016/j.jep.2009.02.040
BadakhshanMP, Lachimanan YL, Sreenivasan S. 2011. Cytotoxicity and oral acute toxicity studies of Lantana camara leaf extract. Molecules 16, 3663–3674.
Baumber J, Ball BA, Gravance CG, Medina V, Daviesmorel MC. 2000. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. Journal of Andrology 21, 895-902. https://doi.org/10.1002/j.19394640.2000.tb03420.x
Bishop ON. Statistics for Biology.1st Edition. Longmans Green and company, London.
Blanco R, Rengifo CE, Cedeño M, Frómeta M, Rengifo E. 2013. Flow Cytometric Measurement of Aneuploid DNA Content Correlates with High S-Phase Fraction and Poor Prognosis in Patients with Non-Small-Cell Lung Cancer. ISRN Biomarkers 354123 (8). https://doi.org/10.1155/2013/354123
Chakrabarti S, Biswas TK, Rokeya B, Ali L, Mosihuzzaman M, Nahar N, Khan A, Azad K, Mukherjee B. 2003. Advanced studies on the hypoglycemic effect of Caesalpinia bonducella F. in type 1 and 2 diabetes in Long Evans rats. Journal of Ethno pharmacology 84, 41–46. https://doi.org/10.1016/S0378-8741(02)00262-3.
Chopra IC, Hauda KL, Kapur LD. 1958. Chopra’s indigenous drugs of India, 2nd Ed. Academic Publishers, India, 304-305.
Datte JY, Yapo PA, Kouamé-Koffi GG, Kati-Coulibaly1 S, Amoikon1 KE, Offoumou AM. 2004. Leaf extract of Caesalpinia bonducella Roxb. (Caesalpiniaceae) induces an increase of contractile force in rat skeletal muscle in situ. Phytomedicine11, 235–241. https://doi.org/10.1078/0944-7113-00292
Garret HE.1956. Elementary Statistics. Longmans Green and Company, London.
Gaur RL, Sahoo MK, Dixit S, Fatma N, Rastogi S, Kulshreshtha DK, Chatterjee RK, Murthy PK. 2008. Antifilarial activity of Caesalpinia bonducella against experimental filarial infections. Indian Journal of Medicinal Research 128, 65-70.
Gupta M, Mazumder UK, Sambath KR, Tangavel TS, Vamsi MLM. 2004. Antitumor Activity and Antioxidant Status of Caesalpinia bonducella against Ehrlich Ascites Carcinoma in Swiss Albino Mice. Journal Pharmacological Science 94, 177–184.
Handa SS, Kaul MK. 1996. Supplement to Cultivation and Utilization of Medicinal Plants. Regional Research Labrotory, India, 727-737.
Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA. 1983.Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. Journal of Histochemistry and Cytochemistry31, 1333–1335. https://doi.org/10.1016/j.phytochem.2008.12.008 https://doi.org/10.3390/molecules16053663.
Jena BS, Das S, Patnaik BK. 1995.Effect of age on lipid peroxidation in a short-lived species of reptile, Calotesversicolor. Archives of Gerontology and Geriatrics 20, 263-272. https://doi.org/10.1016/0167-4943(95)00622-R
Kannur DM, Hukkeri VI, Akki KS. 2006a. Adaptogenic activity of Caesalpinia bonducella seed extracts in rats. Journal of Ethnopharmacology 108, 327–331. https://doi.org/10.1016/j.jep.2006.05.013
Kannur DM, Hukkeri VI, Akki KS. 2006b. Antidiabetic activity of Caesalpinia bonducella seed extracts in rats. Fitoterapia77, 546–549. https://doi.org/10.1016/j.fitote.2006.06.013
Khedkar A, Yuvaraj DM, Shinde G, Khalure P, Dere P.2011. Diuretic effect of Caesalpinia bonduc in rats. Bangladesh Journal of Pharmacology 6, 61–63. https://doi.org/10.3329/bjp.v6i1.8487
Kumar RS, Gupta M, Mazumdar UK, Rejeshwar Y, Kumar TS, Gomathi P, Roy R. 2005. Effect of methanol extracts of Caesalpinia bonducella and Bauhina racemosa on Hematology and Hepatorenal function in mice. Journal of Toxicological Sciences 30, 265-275. https://doi.org/10.2131/jts.30.265
Lilaram, Nazeer AR. 2012. Effect of ethanolic seed extract of Caesalpinia bonducella on female reproductive system of albino rat: a focus on antifertility efficacy. Asian Pacific Journal of Tropical Disease S957-S962. https://doi.org/10.1016/S2222-1808(12)60300-4.
Mandal S, Hazra B, Sarkar R, Biswas S, Mandal N. 2009. Assessment of the Antioxidant and Reactive Oxygen Species scavenging activity of methanolic extract of Caesalpinia crista Leaf. Journal of Evidence based Complementary and Alternative Medicine 10, 1093-1072. https://doi.org/10.1093/ecam/nep072
Moshi MJ, Nagpa V. 2000. Effect of Caesalpinia bonducella seeds on blood glucose in rabbits. Pharmaceutical Biology 38, 81-6. https://doi.org/10.1076/13880209(200004)38211FT081
Nadkarni AK, Nadkarni KR. 1976. Indian Materia Medica, Popular Prakashan, India, 226 – 229 P.
Nadkarni AK. 1954. Indian MateriaMedica. Popular Prakashan. India.
Pearse AGE. 1975. Histochemistry, theoretical and applied. Churchill, Livingstone, London.
Preeja GP, Suresh P. 2011. Evaluation of acute and sub-acute toxicity of methanolic extract of Caesalpinia bonducella (L.). Fleming. European Journal of Scientific Research. 53, 462-69.
Satiyavati GV, Raina MK, Sharma AM. 1976. Medicinal plants of India, Indian council of Medicinal Research, India.
Sestini EA, Carlson JC, Allsopp R. 1991. The Effects of Ambient Temperature on Life Span, Lipid Peroxidation, Superoxide Dismutase, and Phospholipase A2 Activity in Drosophila melanogaster. Experimental Gerontology 26, 385–395. https://doi.org/10.1016/0531-5565(91)90050-V
Sharma SR, Dwivedi SK, Swarup D. 1997. Hypoglycaemic. Antihyperglycaemic and hypolipidemic activities of Caesalpinia bonducella seeds in rats. Journal of Ethnopharmacology58, 39–44. https://doi.org/10.1016/S0378-8741(97)00079-2
Shukla S, Mehta A, John J, Singh S, Mehta P, Vyas SP. 2009b. Antioxidant activity and total phenolic content of ethanolic extract of Caesalpinia bonducella seeds. Food and Chemical Toxicology 47, 1848-1851. https://doi.org/10.1016/j.fct.2009.04.040.
Shukla S, Mehta A, Mehta P, Vyas SP, Shukla S, Bajpai VK. 2010. Studies on anti-inflammatory, antipyretic and analgesic properties of Caesalpinia bonducella F. seed oil in experimental animal models. Food and Chemical Toxicology 48, 61–64. https://doi.org/10.1016/j.fct.2009.09.015.
Shukla S, Mehta A. Jinu J, Mehta P, Vyas SP, Shukla S. 2009a. Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. Journal of Ethnopharmacology 125, 252–256. https://doi.org/10.1016/j.jep.2009.07.002.
Singh V, Raghav KP. 2012. Review on pharmacological properties of Caesalpinia bonducella L. International Journal of Medicinal and Aromatic Plants 2, 514-530.
Sukhdev A, Dhondiram MY, Khalure PR, Balasaheb CN. 2011. Antifertility activity of root bark of Caesalpinia bonducella Roxb. In female albino rats. Pharmacologyonline.3, 34-41.
Wyrobek AJ, Bruce WR. 1975. Chemical induction of sperm abnormality in mice. Proceedings of the National Academy of Sciences 72, 4425-4429.
Yadav PP, Maurya R, Sarkar J, Aeora A, Kanojiya S, Sinha S. 2009. Cassane diterpenes from Caesalpinia bonducella. Phytochemistry 70, 256-261.
Bibarani Tripathy, Surya N. Swain, Manoj K. Panda, Rabi N. Pradhan, Usha R. Acharya (2018), Antispermatogenic effects of seed extract of Caesalpinia bonducella in Swiss mice; IJB, V12, N4, April, P23-34
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